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Citations for GE Life Sciences :
1 - 50 of 726 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
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bioRxiv - Immunology 2019Quote: ... Both mutants carry a carboxy-terminal cmyc-peptide extension and a poly-His peptide extension for purification using nickel columns from GE Healthcare (Chicago, IL) and for anchoring in ELISAs as previously described and specified below (11–15).
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
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bioRxiv - Biochemistry 2021Quote: HR1 with C-terminal poly-his tag was immobilized onto a NTA sensor chip (from GE Healthcare) to around 500 RU using a Biacore SPR 8K (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Biochemistry 2020Quote: ... which utilized the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S20010/300 column ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... The coding sequences for the N-terminal (63-139 aa) and C-terminal (221-307 aa) domain of OR were cloned to the pGEX-4T-1 vector (GE healthcare) for prokaryotic expression ...
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bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
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bioRxiv - Biochemistry 2021Quote: ... which was then used to capture purified HexaPro S2 by the c-terminal twin StrepTag to ~80 response units (RU) in each cycle using a Biacore X100 (GE Healthcare). The binding surface was regenerated between cycles using 0.1% SDS followed by 10 mM Glycine at pH 2 ...
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bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... SC and the D1 domain of FcμR was first immobilized on the chip: SC was biotinylated at the C-terminal and immobilized on the SA chip (GE Healthcare), whereas FcμR-D1 was immobilized on the CM5 chip to a level of resonance units similar to pIgR ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Biochemistry 2019Quote: ... containing an N-terminal PreScission protease (GE Healthcare) cleavable His6-tag prior to MCS 1 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified via a two-step chromatography process involving nickel-affinity chromatography via the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S200 10/300 GL column ...
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bioRxiv - Plant Biology 2023Quote: ... pGEX-2T (GE Healthcare, N-terminal fusion with GST) was used for CPK protein production in bacteria for previously reported constructs72 (CPK2/3/5/11) ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were separated using a Superdex Peptide 3.2/300 column (GE Healthcare) at a flow rate of 10 μl/minute ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Eluted peptides were fractionated using a Superdex Peptide 3.2/300 column (GE Healthcare) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Cell Biology 2020Quote: NRVMs at a confluence of 50% were transfected with either control non-targeting siRNA or pooled siRNA targeting both AMPKα1 and AMPKα2 catalytic isoforms (AMPKα1/α2 siRNA) (from GE Healthcare 50nM) using lipofectamine RNAimax transfection reagent (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted peptides were fractionated on a Superdex Peptide 3.2/300 increase column (GE Healthcare) at a flow rate of 10 µL/min using 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted peptides were fractionated on a Superdex Peptide 3.2/300 increase column (GE Healthcare) at a flow rate of 10 µL/min using 30% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA isoforms were visualised and quantified using an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Bio-Sciences AB). The RiboRuler High Range RNA Ladder (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: EDC-crosslinked FERRY peptides were separated on a Superdex Peptide PC3.2/30 column (GE Healthcare) at a flow rate of 50 µL min−1 ...
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bioRxiv - Cancer Biology 2022Quote: Size based fractionation of the peptides were done using Superdex Peptide 3.2/300 (GE Healthcare) column and HPLC (Agilent 1260 Infinity II) ...
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bioRxiv - Neuroscience 2020Quote: ... Northern blot probes to detect different isoforms of homothorax were prepared by Amersham Megaprime DNA Labeling System (GE Healthcare Life Sciences), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by digestion with thrombin (GE Healthcare) by adding the enzyme at a concentration of 10 Units per mg of KsgA protein during overnight dialysis against PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography on a Superdex Peptide PC 3.2/30 column (GE Healthcare) using water/acetonitrile/TFA (77.4/22.5/0.1 ...
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bioRxiv - Biophysics 2021Quote: ... Peptides were desalted on a C18 MacroTrap column (Michrom Bioresources) and fractionated on a Superdex Peptide (GE Life Sciences) size-exclusion column ...
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bioRxiv - Biochemistry 2019Quote: ... 50 μg of peptides were fractionated in a Shimadzu HPLC system using a Superdex Peptide 3.2/300 (GE Healthcare) at a flow rate of 50 μl/min using SEC buffer (30% (v/v ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.1% FA (Fluka) and crosslinked peptides were enriched by separation on a Superdex Peptide PC 3.2/30 column (GE Healthcare), using an Agilent 1100 chromatography system at 95 μL/minute with fraction collection ...
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bioRxiv - Molecular Biology 2021Quote: ... in water /0.1% (v/v) trifluoroacetic acid (TFA) and crosslinked peptides were enriched by peptide size exclusion chromatography (SuperdexPeptide 3.2/300 column, GE Healthcare) with a flow rate of 50 μl/ min in 30 % (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... a Superdex Peptide 3.2/300 column (GE Healthcare) with 30% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... using a Superdex Peptide 3.2/300 (GE Healthcare) at a flow rate of 10 ţl min1 using 30% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... A Superdex Peptide 3.2/300 column (GE Healthcare) was used for SEC fractionation by isocratic elution with 30% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... a Superdex Peptide 3.2/300 column (GE Healthcare) with 30% (v/v ...
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bioRxiv - Immunology 2023Quote: ... that had encoded an N-terminal His6-tag followed by a PreScission protease (GE Healthcare) cleavage site ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL of this peptide solution was subjected to SEC analysis by passing through Superdex peptide 10/300 GL column (GE healthcare) connected to Bio-Rad (Biologic Duoflow ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were eluted and fractionated by size exclusion chromatography (SEC) using a Superdex Peptide 3.2/300 column (GE Healthcare, Chicago, IL, USA) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... was cloned into the pGEX-6P-1 vector with an N-terminal GST tag (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... with an N-terminal glutathione transferase (GST) tag followed by a PreScission Protease site (GE Healthcare). The codon optimized cDNA for the ATAD2 BRD was designed by BioBasic and subsequently amplified by PCR and cloned into the pDest15 vector ...
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal His6-tag was then removed by digestion with thrombin (≈ 1 U/mg; GE Healthcare) at 4°C overnight ...
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bioRxiv - Biophysics 2021Quote: ... using a Superdex™ Peptide 3.2/300 column (GE Healthcare) at a flow rate of 50 µL/min of the mobile phase (water/acetonitrile/trifluoroacetic acid 70 %/30 %/0.1 % ...