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Citations for GE Life Sciences :
1 - 50 of 3639 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The supernatant was loaded into gravity column containing anti-FLAG M2 affinity gel (GE Healthcare). The resin was washed with wash buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D534 was also purified using 1 mL of M2 anti-FLAG affinity column (GE healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... Samples were purified using nickel-affinity chromatography followed by gel filtration using a Superdex-200 gel-filtration column (GE Healthcare), equilibrated in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The treated sample was further analyzed by affinity chromatography and gel-filtration column (GE Healthcare). Finally ...
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bioRxiv - Genetics 2022Quote: ... Mutant and wild-type DASH complexes were first purified via affinity chromatography using either FLAG resin (GE Healthcare Bioscience) or Nickel resin (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was harvested and the protein was purified by FLAG-affinity purification followed by MonoQ anion exchange chromatography (GE Healthcare). Finally ...
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bioRxiv - Immunology 2022Quote: ... anti-PD-L1 and LH02 were all purified by affinity chromatography using a protein A affinity column (GE Healthcare, Piscataway, NJ, USA) and analyzed in reducing condition on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified through nickel-agarose affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare). The purified protein was concentrated to 30 mg/mL in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested and purified by Ni-NTA affinity chromatography and gel filtration (Superdex 200, GE Healthcare, USA) and eluted in PBS (pH 7.2) ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Neuroscience 2020Quote: ... was used for the affinity purification and Gel filtration was performed on HiLoad 16/60 Superdex 200 (GE Healthcare). Fractions were examined on SDS-PAGE gel before pooling ...
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bioRxiv - Genetics 2021Quote: ... or anti-Flag (M5, Sigma; secondary antibody, NA931V, GE healthcare). Anti-Mam1 (ab155786) ...
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bioRxiv - Microbiology 2020Quote: ... Affinity-purified ComGCSS was further purified by gel-filtration chromatography on an HiLoad 16/600 Superdex 75 column (GE Healthcare), using (25 mM Na2HPO4/NaH2PO4 pH 6 ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoblot membranes were probed with HRP-conjugated affinity-purified donkey anti-mouse or anti-rabbit IgG (GE Healthcare) as secondary antibodies and visualized with the immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Proteins were purified by affinity chromatography using a GSTrap HP affinity column (GE Healthcare) and size exclusion chromatography (HiLoad S200 ...
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bioRxiv - Biophysics 2019Quote: ... it was purified on a nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was further purified using reverse affinity chromatography on Ni-Sepharose followed by gel filtration (Superdex 200 16/60, GE Healthcare). Protein in gel filtration buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... Cleaved proteins were subsequently purified from the protease and affinity tag by a second Ni2+-affinity column or by gel filtration on a Superdex 200 Increase 10/300 GL column (GE Healthcare). Proteins were concentrated to 3–10 mg/ml and either used immediately or aliquoted and quickly frozen at –80°C ...
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bioRxiv - Immunology 2020Quote: ... was further purified by cation exchange and heparin affinity chromatography prior polishing by gel filtration through a Superdex 200 16/40 column (GE Healthcare), which was operated in 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... U-13C-enriched proteins were purified through Ni-NTA affinity column and further by gel filtration chromatography on an ÄKTA Pure 25 FPLC (GE Healthcare) using Superdex 75 Increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting compounds were purified from cell culture supernatants using an kta Express chromatography system using affinity purification using Mabselect Sure Protein A resin followed by a gel filtration column based on the Superdex200 resin (GE Healthcare). The MabSelect Sure Protein A column was washed with 0.1 M Hepes ...
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bioRxiv - Molecular Biology 2023Quote: ... it was purified using nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was affinity-purified using Ni-Ni-Sepharose High-Performance Affinity Matrix (GE Healthcare Biosciences) and eluted using 500mM imidazole containing buffer ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... Clarified lysates were subjected to affinity chromatography using a GSTrap HP affinity column (GE Healthcare). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... SidK1-278-3×FLAG was further purified with a Superdex 200 10/300 Increase gel filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... OCP-CAN was firstly purified by a Ni-NTA affinity chromatography (HisTrap affinity column, GE Healthcare), yielding a mixture of apo-and holo-OCP protein ...
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bioRxiv - Biochemistry 2020Quote: ... A nickel-affinity column (GE Healthcare) was equilibrated with buffer A containing 10mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein from this affinity step was concentrated and further purified by gel filtration on a HiLoad® 16/600 Superdex® 200 pg column (GE Healthcare) equilibrated in 20 mM TrisHCl ...
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bioRxiv - Biochemistry 2023Quote: ... mIgA-604 were purified by anti-light chain affinity chromatography using Capto-L beads (GE Healthcare). dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were purified using Affinity chromatography purification on Glutathione Sepharose 4B beads affinity column (GE Healthcare). After that ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
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bioRxiv - Immunology 2021Quote: ... and 5 mL affinity columns (GE Healthcare) on an AktaPure chromatography system ...
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bioRxiv - Cell Biology 2023Quote: ... affinity purified using glutathione-Sepharose (GE Healthcare), and used to raise antibodies in rabbits following our established protocol (Page et al. ...
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bioRxiv - Biophysics 2020Quote: ... proteins were affinity purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). Supernatant was two-fold diluted with 1xPBS (pH 7.4 ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Microbiology 2019Quote: ... Anti-CFP polyclonal antibodies were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare) according to manufacturer instructions.
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Bioengineering 2021Quote: Affinity of IDO-Gal3 for lactose was determined using affinity chromatography in an AKTA Pure chromatography system (GE Life Sciences) equipped with consumer-packable glass column (GE Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... was expressed in E.Coli Rosetta 2 (DE3) and purified by affinity chromatography over a Sepharose 4B GSH affinity column (GE healthcare) followed by gel filtration over a Hiload Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Ni-NTA affinity (on HisTrap column, GE Healthcare) and ion exchange (ResourseQ column ...
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bioRxiv - Molecular Biology 2022Quote: ... or heparin affinity (GE Healthcare HiTrap-Heparin; Spt6) chromatography was used to purify 10xHis-mClover-Cdc73 ...
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bioRxiv - Immunology 2020Quote: ... and purified through HisTrap affinity chromatography (GE Healthcare) and size exclusion chromatography (Superdex 75 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... and purified by KappaSelect affinity chromatography (GE Healthcare), followed by size exclusion chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... and purified via KappaSelect affinity chromatography (GE Healthcare), cation exchange chromatography (MonoS ...
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bioRxiv - Biochemistry 2021Quote: ... followed by heparin affinity chromatography (GE Healthcare, US). The peak fractions containing the target proteins were pooled ...
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bioRxiv - Microbiology 2020Quote: ... and purified via KappaSelect affinity chromatography (GE Healthcare), cation exchange chromatography (MonoS ...
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bioRxiv - Biochemistry 2022Quote: ... ExoU was purified by an initial affinity step (immobilised nickel affinity chromatography) followed by size-exclusion chromatography (SEC) (16/600 Superdex 200, GE healthcare) in 20 mM Tris–HCl pH 8.2 ...