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Citations for GE Life Sciences :
1 - 50 of 4956 citations for 8 HYDROXY 1 3 DIOXOLO 4 5 G QUINOLINE 7 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...
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bioRxiv - Immunology 2023Quote: ... A 5 ml protein G Sepharose 4 Fast flow column (GE Healthcare) was used to extract total human IgG ...
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bioRxiv - Cell Biology 2019Quote: ... and 3’-primary amino-modified miR-430 MO (5’-TCTACCCCAACTTGATAGCACTTTC-3’) was obtained from Gene tools LLC and labeled with Cy3 NHS-ester (GE Healthcare). After the conjugation for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... for 1-7 days before acquiring radiograms using Typhoon 5 Imager (GE Healthcare). Densitometry was carried out using ImageJ53 ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Immunology 2020Quote: Columns of 1 ml Protein G sepharose 4 fast flow (GE Healthcare) were prepared and equilibrated with 5 volumes of binding buffer (0.02 M sodium phosphate ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... were pooled and coupled with either Cy®3 Mono NHS Ester (GE healthcare) or Alexa FluorTM (AF ...
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bioRxiv - Microbiology 2022Quote: ... pH 8 using MiniTrap G-25 desalting columns (GE Healthcare) and stored with protease inhibitor at −20°C until use.
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Pathology 2019Quote: ... Electrophoresis was performed at 100V (200 mA) for 1 h and 150V (300 mA) for 7-8 hrs in Ettan DALT six apparatus (GE Healthcare). After electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Immunology 2022Quote: ... to 3 mL for loading onto a 1 × 66 cm gel filtration column comprised of 4 g of degassed Sephadex G-50 beads (GE Healthcare) preequilibrated in buffer A O/N ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2019Quote: ... Virus labeling was performed with Alexa Fluor™A647 succinimidyl ester in a 1:10 molar ratio and was then purified through Nap-5 desalting column (GE Healthcare). Labeled viruses were aliquoted and stored at −80°C ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... This was then coupled onto NHS-ester Sepharose 4 Fast Flow resin (GE Healthcare, 17-0906-01) for 4 h at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Genetics 2023Quote: ... Protein G Sepharose 4 Fast Flow (GE Healthcare, 17061801) was equilibrated with PBS containing 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed using 24 cm 3–7 NL Immobiline DryStrip (GE Healthcare) in an IPGphor unit (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... placed on Ficoll-Paque Plus (ratio 7:3) (GE Healthcare Life Sciences, 17144003), and centrifuged for 20 min at 1800 rpm at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Neuroscience 2022Quote: ... Aβ42 peptides in the filtrate were further concentrated to ∼50 μM at 4°C and 4000×g with a 5 000 Da Vivaspin concentrator (GE Healthcare). The Aβ42 peptide concentration was calculated using an extinction coefficient of 1400 M-1cm-1 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were filtered and then pelleted at 300 g for 5 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were precleared using 20µl of protein G sepahrose beads (GE Healthcare, 7-0618-01) for 1hr at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Cy3B NHS ester (GE Healthcare); ATTO 647N NHS ester (ATTO-TEC) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... see Figure 3 for detergent content) was added to 10 μl of packed beads: Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Bioengineering 2021Quote: ... a Sephadex G-25 column (GE Healthcare, NAP-5) was equilibrated with PBS (PH 7.4) ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...