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Citations for GE Life Sciences :
1 - 50 of 3572 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... pH 8 using a PD-10 desalting column (GE Healthcare) and further diluted to a final volume of 20 ml using 10 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2020Quote: ... pH 8) and loaded on a Superdex 75 10/300 GL (GE Healthcare) size exclusion column to remove s10 ...
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bioRxiv - Biochemistry 2020Quote: ... 20 mM Tris-HCl pH 8 using PD-10 desalting columns (GE Healthcare). 10 μM CAD was hydroxylated by incubation with 10 μM FIH ...
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bioRxiv - Biochemistry 2021Quote: ... pH 8 supplemented with 0.22 mM DDM by gel filtration using the PD-10 column (GE Healthcare). Samples were concentrated using Amicon®Ultra-4 ultrafiltration device ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: ... The 100-µl sample (8 mg/ml) was loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with filtered and degassed SEC buffer containing 3% glycerol and 5 mM dithiothreitol instead of MgCl2 and β-mercaptoethanol ...
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bioRxiv - Biophysics 2021Quote: ... 0.5 M NaCl pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biophysics 2022Quote: ... 180 µL of approximately 8 µM sample was injected onto a Superose6 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM TrisHCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Peak TopBP1b6-8 fractions (eluted with 50mM and 75mM imidazole) were desalted (HiTrap™ or HiPrep 26/10, GE Healthcare) in physiological buffer (10mM Na2H/NaH2 PO4 pH 7.6 ...
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bioRxiv - Genomics 2020Quote: ... Equivalent amounts of protein were resolved by 8-10% SDS-PAGE and blotted onto nitrocellulose (0.2 µm, Amersham Protran) with a semi-dry blotter (GE Healthcare). The membrane was stained with Ponceau S solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Biochemistry 2019Quote: SEC-MALS was conducted using a Wyatt Dawn 8+ detector (Wyatt Technology) coupled to a Superdex 75 10/300 column (GE Healthcare) and a refractive index detector ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were separated by denaturing (8 M urea) 10% PAGE and were visualized by using a Typhoon phosphorimager (GE Healthcare). As described previously,48 band intensities were determined and used to estimate the fraction of RNAs bound to ligand ...
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bioRxiv - Biophysics 2020Quote: ... eluate fractions from the Ni2+-NTA column containing the nsp7-8-His6 were concentrated and the buffer exchanged with a PD-10 desalting column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: Affinity-purified xtZnT8 was concentrated to 6-8 mg/ml and loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), and was further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Biochemistry 2023Quote: ... a final gel filtration step was performed in GF buffer (30 mM HEPES pH 8, 250 mM NaCl, 10 mM TCEP) using a S200 size exclusion chromatography column (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... Proteins were dialyzed against 10 mM Tris (pH 8) and purified by ion exchange (Q column) followed by size exclusion (S200, GE Healthcare) chromatography ...
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bioRxiv - Plant Biology 2023Quote: ... gel analysis were pooled and dialyzed against Tris-HCl buffer (30 mM Tris-HCl, pH 8) using PD-10 Desalting Columns (GE Healthcare). The protein concentration was determined by Bradford assays with BSA (bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was neutralized by addition of 1:10 vol:vol 1M Tris pH 8 and applied to a 5 mL protein A Hitrap FF column (GE Healthcare Life Sciences) equilibrated in buffer A (100 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... Equal amounts of protein (10-50 µg) from the total extract were separated on 8-10% SDS-polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Germany) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at 8-13 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at concentrations >8 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... 8 mM of NHS-Cy5 (GE Healthcare) or NHS-Sulfo-Cyanine 7 (Lumiprobe ...
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... was analyzed by electrophoresis on a denaturing 8% polyacrylamide gel containing 8 M urea followed by transfer to a positively-charge Hybond nylon membrane (GE Healthcare). Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol ...
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bioRxiv - Biochemistry 2020Quote: ... Nickelnitrilotriacetic acid-agarose was from GE Healthcare. Deidentified ...
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bioRxiv - Biochemistry 2020Quote: ... and quantified using ImageQuant TL 8 software (GE Healthcare). Comparative analysis of numerous tRNA and enzyme variants was performed using single-turnover rate conditions with the concentration of Hs ProXp-ala several fold smaller than the apparent Kd ...
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bioRxiv - Biochemistry 2019Quote: ... PreScission proteinase (8 units/mg of protein, GE Healthcare) was added to remove the His6 tag from the Asf1 ...
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bioRxiv - Plant Biology 2020Quote: ... [8-3H]-hypoxanthine (925 GBq/mmol; GE Healthcare UK Limited); trans-[2-3H]-zeatin (592 GBq/mmol ...
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bioRxiv - Microbiology 2022Quote: ... pH 8 using MiniTrap G-25 desalting columns (GE Healthcare) and stored with protease inhibitor at −20°C until use.
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bioRxiv - Immunology 2021Quote: ... and 1X non-essential amino acids (HyClone, GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... and 1X non-essential amino acids (HyClone, GE Healthcare). T cells were activated for 2 days with anti-CD3/CD28 magnetic dynabeads (Thermo Fisher ...
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bioRxiv - Pathology 2019Quote: ... 5ug/mL Polyadenylic acid (GE Healthcare 27-4110-01), 2.5ug/mL pd (t)12-18 (Oligo dT ...
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bioRxiv - Pathology 2023Quote: ... packed with Ni2+ nitrilotriacetic acid (NTA)-sepharose (GE Healthcare) were used ...
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bioRxiv - Physiology 2020Quote: ... non-essential amino acids (GE Healthcare HyClone Laboratories, Logan, UT), 1X Penicillin/Streptomycin and Gentamicin (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Microbiology 2023Quote: ... 8 µl of SpeedBeads™ magnetic carboxylate modified particles (50% GE Healthcare 45152105050250 and 50% Thermo Scientific 65152105050250 after two times washing with milli-Q water ...
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bioRxiv - Neuroscience 2023Quote: SPR data were collected on a Biacore 8 K device (GE Healthcare). Antibody (6A3 ...
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bioRxiv - Bioengineering 2023Quote: ... with an 8-channel cardiac phased-array coil (GE Healthcare, Waukesha, WI). Volunteers performed tidal breathing in a supine position ...
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bioRxiv - Biochemistry 2020Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was purchased from GE Healthcare. The plasmid encoded an N-terminal 8X His-SUMO-tagged hNatD in the pET-21a vector was obtained from Genscript ...
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bioRxiv - Cell Biology 2021Quote: ... FBS and MEM Non-essential amino acids (NEAA) from GE Healthcare Life Sciences (Logan ...