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Citations for GE Life Sciences :
1 - 50 of 133 citations for 7 Chlorotryptamine HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... gel analysis were pooled and dialyzed against Tris-HCl buffer (30 mM Tris-HCl, pH 8) using PD-10 Desalting Columns (GE Healthcare). The protein concentration was determined by Bradford assays with BSA (bovine serum albumin ...
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bioRxiv - Microbiology 2020Quote: ... 7 cm strips (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... (7) Superdex 75 resin (GE Healthcare) chromatography using an isocratic elution in Tris buffer B and (8 ...
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bioRxiv - Biochemistry 2020Quote: ... and ethanolamine-HCl were purchased from GE Healthcare (Piscataway, NJ, USA). NeutrAvidin was purchased from Life Technologies Corporation (Grand Island ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Cell Biology 2021Quote: ... A regeneration test was performed using 10mM glycine-HCl pH 2.1 (GE healthcare), 10mM glycine HCl pH2.1 ...
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bioRxiv - Biochemistry 2020Quote: ... 20 mM Tris-HCl pH 8 using PD-10 desalting columns (GE Healthcare). 10 μM CAD was hydroxylated by incubation with 10 μM FIH ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... pH 7) using a PD-10 column (GE Healthcare). Precipitated material was removed by centrifugation at 10,000 × g for 10 minutes and soluble protein were diluted or concentrated to ∼2.4 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Molecular Biology 2021Quote: ... The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare). Recombinant Pfexp-2/Hb at increasing concentrations was injected over the immobilized HDP and on the reference flow cell at a flow rate of 20 μl min−1 ...
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bioRxiv - Microbiology 2021Quote: ... Data were collected using Unicorn 7 software (GE Healthcare Biosciences).
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bioRxiv - Evolutionary Biology 2019Quote: ... We used a Vivid 7 echocardiographic system (GE Healthcare, USA) with an 11 MHz phased array pediatric transducer operating at a frame rate of 60 Hz ...
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bioRxiv - Microbiology 2021Quote: ... and regeneration was performed in 10mM Glycine HCl (pH 1.5, GE Healthcare, Chicago, IL, BR-1003-54). Binding-affinity constants (KD ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
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bioRxiv - Plant Biology 2021Quote: ... The pellets were resuspended in buffer containing 7 M urea (GE Healthcare), 2 M thiourea (GE Healthcare) ...
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bioRxiv - Cancer Biology 2019Quote: ... and 7 μL samples were spotted on Whatman DE81 paper (GE Healthcare). The unincorporated nucleotides were washed four times with 0.5 M Na2HPO4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Using a Vivid 7 echocardiography device (GE Healthcare Life Sciences, Chalfont, UK) and a 12-MHz linear transducer ...
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bioRxiv - Biochemistry 2021Quote: ... equilibrated with Tris-HCl buffer (50 mM, pH 8.5) and connected to an Äkta Xpress system (GE Healthcare). Elution was performed by applying a linear gradient from 0 to 500 mM NaCl (in Tris-HCl buffer 50 mM ...
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bioRxiv - Microbiology 2020Quote: ... 20 mM Tris HCl pH 8.0) using Vivaspin filters with a 100 kDa molecular weight cutoff (GE Healthcare). Protein concentrations were determined using the Nanodrop method ...
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bioRxiv - Microbiology 2022Quote: ... adjusted to pH 8 with HCl) and applied to an HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The buffer was exchanged in assay buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1 % Tween-20) using PD10 gravity columns (GE Healthcare). The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed using 24 cm 3–7 NL Immobiline DryStrip (GE Healthcare) in an IPGphor unit (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed in 24 cm 4–7 NL Immobiline DryStrip (GE Healthcare) in an IPGphor unit (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... placed on Ficoll-Paque Plus (ratio 7:3) (GE Healthcare Life Sciences, 17144003), and centrifuged for 20 min at 1800 rpm at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and COS-7 cells were grown in high-glucose DMEM (GE Healthcare) supplemented with 10% bovine calf serum (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... for 1-7 days before acquiring radiograms using Typhoon 5 Imager (GE Healthcare). Densitometry was carried out using ImageJ53 ...
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bioRxiv - Immunology 2021Quote: ... Cardiac function was measured by Vivid 7 ultrasound machine (GE Healthcare, Pittsburgh, PA) and 13-MHz linear array transducer ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... Images were acquired using a Vivid 7 Dimension system (GE Healthcare, Horten, Norway) and a i13L probe (10-14 MHz ...
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bioRxiv - Cell Biology 2020Quote: ... was exchanged into labelling buffer (20 mM Tris-HCl pH 7.5, 100 mM NaCl) using size exclusion NAP10 columns (GE Healthcare). Protein-containing fractions were identified by protein electrophoresis and pooled ...
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bioRxiv - Microbiology 2019Quote: ... Purified protein was immediately exchanged into TEV cleavage buffer (50 mM Tris-HCl pH 8.0, 10% glycerol) using a 50 ml desalting column (GE Healthcare). For His-tag cleavage ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 3.0 (pH neutralization to 7.8 with 1 M Tris-HCl, pH 9.0) using ÄKTA FPLC system (GE Healthcare Life Sciences). The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore) ...
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bioRxiv - Cell Biology 2019Quote: ... adjusted to 100 mM Tris-HCl pH 8.0 and 1 mM EDTA and loaded onto a StrepTrap HP column (GE Healthcare) pre-equilibrated in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare). Fractions were collected and stored at 4°C.
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bioRxiv - Biochemistry 2021Quote: ... The periplasmic extract was adjusted to 50 mM Tris/HCl pH 9.0 (loading buffer) and loaded onto a 5 mL Q Sepharose anion exchange column (GE Healthcare). Proteins were eluted using a linear salt gradient (0-500 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified proteins were dialyzed against TN200 buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl) using a HiTrap Dessalting column (GE Healthcare), and the protein was further purified by gel filtration chromatography on a HiLoad 16/600 Superdex 75 or HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... in a high-salt buffer containing 20 mM Tris-HCl (pH 8.0) and 50 mM NaCl in an ÄktaPrime or ÄktaExplorer system (GE Healthcare). The column was calibrated with bovine erythrocyte carbonic anhydrase ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2019Quote: ... 25 mM Tris-HCl pH 7.5) prior to adding to a HiTrap QFF (1 mL) strong anion exchange column (GE Healthcare) equilibrated with 10 column volumes (CV ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved protein was diluted with 20 mM Tris-HCl pH 7.5 to reach 150 mM NaCl and loaded onto pre-equilibrated HiTrap Q HP column (GE Healthcare). The column was washed with 200 mM NaCl Buffer R (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded complexes were further purified in a final size exclusion chromatography step (20 mM Tris-HCl pH 8.5, 150 mM NaCl) using a Superdex 200i column (GE Healthcare), before being used in down-stream applications.
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bioRxiv - Biochemistry 2019Quote: ... The total eluate was diluted 1:2 with 25 mM Tris-HCl pH 8.0 and further purified by anion exchange chromatography using a 6 ml RESOURCE Q column (GE Healthcare) equilibrated in 95% AEX-A (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... Obtained PAR was diluted with 20mM Tris-HCl buffer pH 7.5 and was loaded into Resource Q anion exchange 1ml column (GE Healthcare) for fractionation with a linear gradient of 0 - 1M NaCl at 0.25ml/min flow rate ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 10 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and passed over a Q-sepharose FF (GE Healthcare) column ...