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Citations for GE Life Sciences :
1 - 50 of 2149 citations for 7 AMINONAPHTHALENE 1 3 DISULFONIC ACID POTASSIUM SALT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed using 24 cm 3–7 NL Immobiline DryStrip (GE Healthcare) in an IPGphor unit (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... placed on Ficoll-Paque Plus (ratio 7:3) (GE Healthcare Life Sciences, 17144003), and centrifuged for 20 min at 1800 rpm at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... were loaded on 7-cm long immobilized pH gradient (IPG) strips with non-linear (NL) 3-10 pH gradient (GE Healthcare). Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... for 1-7 days before acquiring radiograms using Typhoon 5 Imager (GE Healthcare). Densitometry was carried out using ImageJ53 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... 7 cm strips (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... (7) Superdex 75 resin (GE Healthcare) chromatography using an isocratic elution in Tris buffer B and (8 ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Bioengineering 2020Quote: ... disposable PD10 desalting salt columns were purchased from GE Healthcare Life Sciences and Amicon® Ultra centrifugal filters from Merck Millipore.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... was diluted to a final salt concentration of 200 mM NaCl before loading onto a 1 mL heparin column (GE Healthcare) equilibrated in low-salt buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... MBNL1 (amino acids 1-269 of MBNL140) was obtained in pGEX-6P1 (GE Healthcare). Plasmids were transformed into E ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% (v/v) ampholytes (pH 3-11 NL; GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then incubated with [3H]acetic acid (1 μCi/well; Amersham, GE Healthcare) in serum free DMEM F-12 for 4 hrs ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Systems Biology 2019Quote: ... 0.7% (v/v) acetonitrile (ACN) and 1% (v/v) carrier ampholytes mixture (IPG buffer pH 3.0-10.0, GE Healthcare). Fractionation was performed on a 3100 OFFGEL (OGE ...
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bioRxiv - Cell Biology 2020Quote: ... An 11 kb Spe1-Sal1 fragment (mid exon 1–7) was labelled with Cy3-dUTP (GE Healthcare UK Ltd) using Bioprime labelling kit (Invitrogen) ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... pH 7) using a PD-10 column (GE Healthcare). Precipitated material was removed by centrifugation at 10,000 × g for 10 minutes and soluble protein were diluted or concentrated to ∼2.4 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Microbiology 2022Quote: ... Erythrocyte lysis was performed using ammonium-chloride-potassium (ACK) buffer and leukocytes were isolated by gradient centrifugation using Percoll solution (GE Healthcare). Lung tissue leukocytes were filtered again and antibody staining for flow cytometry was performed.
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bioRxiv - Neuroscience 2020Quote: ... in 1X Hank’s Balanced Salt Solution (SH30588.02, GE Healthcare Life Sciences, United States). The dura was removed and the spinal cord was bisected lengthwise ...
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bioRxiv - Neuroscience 2022Quote: ... in Hanks’ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with 20 mg/mL DNase (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Data were collected using Unicorn 7 software (GE Healthcare Biosciences).
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bioRxiv - Evolutionary Biology 2019Quote: ... We used a Vivid 7 echocardiographic system (GE Healthcare, USA) with an 11 MHz phased array pediatric transducer operating at a frame rate of 60 Hz ...
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bioRxiv - Pathology 2019Quote: ... Electrophoresis was performed at 100V (200 mA) for 1 h and 150V (300 mA) for 7-8 hrs in Ettan DALT six apparatus (GE Healthcare). After electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Biochemistry 2020Quote: ... Nickelnitrilotriacetic acid-agarose was from GE Healthcare. Deidentified ...
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bioRxiv - Plant Biology 2021Quote: ... The pellets were resuspended in buffer containing 7 M urea (GE Healthcare), 2 M thiourea (GE Healthcare) ...
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bioRxiv - Cancer Biology 2019Quote: ... and 7 μL samples were spotted on Whatman DE81 paper (GE Healthcare). The unincorporated nucleotides were washed four times with 0.5 M Na2HPO4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Using a Vivid 7 echocardiography device (GE Healthcare Life Sciences, Chalfont, UK) and a 12-MHz linear transducer ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...