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Citations for GE Life Sciences :
1 - 50 of 3483 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... for 1-7 days before acquiring radiograms using Typhoon 5 Imager (GE Healthcare). Densitometry was carried out using ImageJ53 ...
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bioRxiv - Pathology 2019Quote: ... Electrophoresis was performed at 100V (200 mA) for 1 h and 150V (300 mA) for 7-8 hrs in Ettan DALT six apparatus (GE Healthcare). After electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Bioengineering 2021Quote: ... and on one 5 ml StrepTrap HP column (GE Healthcare, US) equilibrated in binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded over one 5 mL HisTrap FF Crude column (GE Healthcare) at 5 mL/min by an AKTA Pure (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was neutralized by addition of 1:10 vol:vol 1M Tris pH 8 and applied to a 5 mL protein A Hitrap FF column (GE Healthcare Life Sciences) equilibrated in buffer A (100 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... at a dilution of 1:10,000 for one hour (GE Healthcare). Proteins were detected using SuperSignal West Femto substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and mCherry (5% laser power, 0,05 exposure) were recorded at 7 minutes intervals and data were analyzed using SoftWoRx (GE Healthcare). The time from nuclear envelope breakdown (NEBD ...
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bioRxiv - Microbiology 2020Quote: ... 7 cm strips (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... The membrane was then incubated with primary antibody diluted in TBST/BSA for one hour followed by a one hour incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:10,000, GE Healthcare life sciences, Piscataway, NJ). Primary antibodies used were ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... (7) Superdex 75 resin (GE Healthcare) chromatography using an isocratic elution in Tris buffer B and (8 ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biophysics 2022Quote: All samples were pre-reduced with 5 mM DTT for one hour followed by desalting using a PD10 column (GE Healthcare) into 20 mM tris pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... One CM5 chip (GE Healthcare) flow cell was coated with 275 RUs of anti-Human IgG antibody (Abcam) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Zoology 2021Quote: ... One of the obtained PCR products was purified with ExoProStar 1-Step kit (GE Healthcare US77702) and Sanger-sequenced directly at Macrogen (Netherlands) ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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bioRxiv - Biochemistry 2020Quote: ... 25 mM HEPES/NaOH pH 8) into a 1 mL HiTrap Heparin HP column (GE Healthcare). This column was further washed with buffer NT.A ...
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bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
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bioRxiv - Systems Biology 2019Quote: ... 0.7% (v/v) acetonitrile (ACN) and 1% (v/v) carrier ampholytes mixture (IPG buffer pH 3.0-10.0, GE Healthcare). Fractionation was performed on a 3100 OFFGEL (OGE ...
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bioRxiv - Cell Biology 2020Quote: ... An 11 kb Spe1-Sal1 fragment (mid exon 1–7) was labelled with Cy3-dUTP (GE Healthcare UK Ltd) using Bioprime labelling kit (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... pH 7) using a PD-10 column (GE Healthcare). Precipitated material was removed by centrifugation at 10,000 × g for 10 minutes and soluble protein were diluted or concentrated to ∼2.4 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Biochemistry 2023Quote: ... A MonoS column (5/50 GL GE Healthcare 1 mL) was equilibrated with 2% MonoS B ...
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bioRxiv - Biophysics 2021Quote: ... 8 mM of NHS-Cy5 (GE Healthcare) or NHS-Sulfo-Cyanine 7 (Lumiprobe ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant for each nanobody was then applied to one 1 ml Ni-NTA pre-packed column (GE healthcare) pre-equilibrated with base buffer + 30 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... Data were collected using Unicorn 7 software (GE Healthcare Biosciences).
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bioRxiv - Evolutionary Biology 2019Quote: ... We used a Vivid 7 echocardiographic system (GE Healthcare, USA) with an 11 MHz phased array pediatric transducer operating at a frame rate of 60 Hz ...
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bioRxiv - Neuroscience 2022Quote: ... For AKT1 detection, anti-AKT (pan) (11E7) (Cell Signalling Technology, #4685, 1:1000 in 5% BSA) and donkey-anti-rabbit IG (GE Healthcare, NA9340V, 1:3000 in 5% BSA) antibodies were used ...
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bioRxiv - Molecular Biology 2023Quote: ... one volume of Protein A Sepharose beads (GE Healthcare) was mixed with two volumes of VCF1 N-terminus (1 mg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... was analyzed by electrophoresis on a denaturing 8% polyacrylamide gel containing 8 M urea followed by transfer to a positively-charge Hybond nylon membrane (GE Healthcare). Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare), which was equilibrated with MonoQ buffer A containing 50 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT and 5 ml of Q Sepharose resin (GE Healthcare) was mixed to capture PGL-3 protein molecules ...
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bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in 1 × TBST with 5% non-fat milk: anti-rabbit IgG (1:10,000; NA934V; GE Healthcare) and anti-mouse IgG (1:10,000 ...
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bioRxiv - Physiology 2022Quote: ... membranes were washed three times with 1X TBST and incubated with secondary antibodies in 5% milk in TBST for one hour at room temperature with the following concentration of secondary antibodies: donkey anti-rabbit IgG (1:5000, NA934, GE Healthcare) and goat anti-mouse IgG (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... and quantified using ImageQuant TL 8 software (GE Healthcare). Comparative analysis of numerous tRNA and enzyme variants was performed using single-turnover rate conditions with the concentration of Hs ProXp-ala several fold smaller than the apparent Kd ...