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Citations for GE Life Sciences :
1 - 50 of 1348 citations for 6 Ethoxypyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: For amine coupling using the Amine Coupling kit (GE Healthcare), ligand proteins were desalted into immobilization buffer (25 mM potassium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Bioengineering 2020Quote: ... using the amine coupling kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and Amine coupling kit (GE Healthcare, USA) were used for immobilization of SARS-CoV-2 Spike S1+S2 ECD-His recombinant protein (Sino Biological ...
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bioRxiv - Bioengineering 2023Quote: ... using amine coupling kit (GE Healthcare, USA). First ...
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bioRxiv - Microbiology 2021Quote: ... CM5 chip was first coated with streptavidin by standard amine coupling using an amine coupling kit (Biacore, GE Healthcare), followed by immobilization of biotin-heparin (38) ...
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bioRxiv - Plant Biology 2020Quote: ... using an amine coupling method kit (GE Healthcare). A blank immobilization was performed in following the same method and was taken as the reference surface ...
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bioRxiv - Neuroscience 2019Quote: ... Each probe contains a 3’-end amine-modified nucleotide that allows directly couple to an NHS-ester Cy3 dye (GE Healthcare, PA23001) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... by an Amine Coupling Kit (GE Healthcare, BR-1000-50). The interaction between CyPA and CD147 was detected using the kinetic analysis/concentration series/direct binding mode ...
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bioRxiv - Microbiology 2021Quote: ... proteins were captured using an amine coupling kit (GE Healthcare), in which the carboxylmethyl dextran matrix of the sensor chip was activated by injection of a mixture of 0.2M 1-ethyl-3-[(3-dimethylamino ...
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bioRxiv - Cell Biology 2020Quote: ... were amine coupled on to a Series S CM5 sensor chip (GE Healthcare) using 2 adjacent flow cells (i.e ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2021Quote: ... followed by concentrating the sample (VivaSpin 6 MWCO 3 kDa cut-off, GE Healthcare) to 8 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Cy3 or Cy5 amine-reactive dyes (Amersham CyDye Mono-Reactive Dye Packs, GE Healthcare) were dissolved in 20 µL DMSO ...
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bioRxiv - Microbiology 2020Quote: ... was immobilized on a CM5 chip using a random amine coupling kit (GE Healthcare) to a final chip density of 3214 and 4559 response units (RU) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification column was prepared using 2-3 mL IMAC Sepharose 6 Fast Flow beads (GE Healthcare) and charged with 0.1 N Nickel sulphate solution ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies were immobilized using direct immobilization using amine coupling on CM3 sensor chips (GE Healthcare) at ∼5000 RU ...
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bioRxiv - Biophysics 2019Quote: ... DNA modified with a primary amine group was mixed with Cy3 NHS ester (GE Healthcare) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... was directly immobilized on the CM5 chip using an amine coupling kit (GE Healthcare Life Sciences). Before immobilization ...
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bioRxiv - Cell Biology 2021Quote: ... was amine coupled to the carboxy-methylated dextran surface of a CM5 sensor chip (GE Healthcare) using standard amine coupling chemistry ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... the sensor chip was saturated with streptavidin using an amine coupling kit (GE Healthcare Life Sciences). Biotinylated pMHCs were immobilize at various levels (100–300 RU for kinetics ...
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bioRxiv - Microbiology 2020Quote: ... sortase and excess DBCO-amine was removed using Ni-NTA resin and PD-10 columns (GE Healthcare) respectively ...
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bioRxiv - Biophysics 2020Quote: ... Neutravidin (Pierce) was amine coupled to the carboxymethylated dextran surface of a CM5 sensor chip (GE Healthcare) using standard amine coupling chemistry ...
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bioRxiv - Biophysics 2020Quote: The SPR chip was prepared by amine coupling of a polyclonal rabbit anti-mouse IgG (GE Healthcare) on both flow cells of CMD500 chip (Xantec) ...
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bioRxiv - Biochemistry 2021Quote: Aβ42 monomers were immobilized by amine coupling onto flowcell 4 on a CM5 sensor chip (GE Healthcare) using a BIAcore 3000 instrument (BIAcore AB) ...
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bioRxiv - Immunology 2022Quote: ... Ligand immobilization was done through amine coupling to an NHS-EDC activated CM5 chip (GE HealthCare, Uppsala, Sweden). CR6261 or CR9114 were immobilized at 1000 RUs in 10 mM Sodium Acetate buffer (pH 4.5 ...
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bioRxiv - Biochemistry 2021Quote: ... was immobilised on a CM5 chip through amine conjugation using NHS/EDC coupling using a Biacore X100 (GE Healthcare). Samples were run at 30 μL/min with an injection time of 60 s and a dissociation of 200 s ...
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bioRxiv - Microbiology 2020Quote: ... pH 4) was covalently immobilized on a CM5 sensor chip using an Amine coupling kit (GE Healthcare Life Sciences). Binding analyses were performed in HBS-P buffer (0.01 M HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... hTau and mTau were immobilised onto an amine-coupling activated (NHS/EDC) CM5 Series S Sensor Chip (GE Healthcare) with a contact time of 180 s at 5 µL/min flow rate and at 25°C ...
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bioRxiv - Immunology 2023Quote: ... Binding responses were measured following immobilization by amine coupling of antigens (37) on CM5 sensor chips (BIAcore/GE Healthcare). Purified plasma IgG samples at 200 µg/ml were flowed (2.5 min ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... Active HAP fractions eluting from 0.2–0.3 M KPi were pooled and separated on a Superose 6 XK 16/70 gel filtration column (130 ml, GE Healthcare) equilibrated with buffer D + 0.1 mM EDTA at 0.15 M KCl ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM NaCl to a final concentration of ∼3 mg/ml further purified by size-exclusion chromatography using a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer ...
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Plant Biology 2022Quote: ... purified PIN1 and sybody-21 were mixed together with a molar ratio of ∼1:3 and then applied to size-exclusion chromatography using a Superose 6 Increase column (GE Healthcare) in buffer containing 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... sfGFP-POX10 was diluted to 5 µg/mL in sodium acetate buffer (pH 4.5) and subsequently covalently immobilized on a CM5 chip by amine coupling according to the manufacturer’ ss protocol (GE Healthcare). Next ...
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bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Developmental Biology 2021Quote: ... pH 4.0 were immobilized on a Biacore CM5 sensor chip (research grade) with the amine coupling kit following the manufacturer’s instructions (GE Healthcare). 1700 resonance units of FBN2-Ct or ADAMTS6-Ct was coupled to the chip for analysis in a Biacore 3000 instrument (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... fragments or Fabs 2/1.12 or 2/6.14 were either used as analytes or amine immobilized on a CM5 chip in a Biacore 3000 (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2006 35 was covalently immobilized on a CM7 Series S sensor chip using an amine coupling reagent kit (GE Healthcare) at the range level of 3,000 to 5,000 response units (RU) ...
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bioRxiv - Cancer Biology 2022Quote: ... a selection of mice were injected intra-peritoneally with 3 rounds of 6 injections each of 200μg poly(I:C) (GE Healthcare, #27-4732-01). Poly(I:C ...
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bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
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bioRxiv - Immunology 2021Quote: ... PPACK-thrombin or fibrinogen in acetate buffer pH4.5 was immobilized to a chip surface (1000 or 10000 RU) using NHS/EDC-amine coupling and residual binding sites blocked by 1M ethanolamine (GE Healthcare). FH (0.5-62nM ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
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bioRxiv - Cell Biology 2019Quote: ... as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare) using an amine coupling kit (GE healthcare). GST tagged ligand proteins were captured on the detection flow cell of the chip and free GST was captured on the reference flow cell ...
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bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Microbiology 2020Quote: ... The modified Cy3 labeled fragment was generated by labeling an amine-modified RNA containing a 5′ monophosphate ordered from Horizon Discovery with monoreactive Cy3 (GE Life Sciences) and the labeled RNA was purified by reverse-phase HPLC ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...