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Citations for GE Life Sciences :
1 - 50 of 2237 citations for 6 CHLORO 2 PIPERIDIN 4 YL 1H BENZO D IMIDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
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bioRxiv - Biochemistry 2021Quote: ... 250 mM imidazole) and subsequently by gel filtration using a Superose 6 16/60 column (GE Healthcare) equilibrated with 50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Biophysics 2020Quote: ... 3mM CaCl2 and 5mM Imidazole pH 8.0 and incubated with Ni2+ charged IMAC Sepharose 6 Fast Flow resin (GE Healthcare) for 1 hr at 25°C ...
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bioRxiv - Biophysics 2021Quote: ... the protein was finally eluted by 230 mM imidazole and further purified through size exclusion chromatography (SEC, Superose 6, 10/300, GE Healthcare) with Lysis Buffer containing 0.001% LMNG ...
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bioRxiv - Cell Biology 2023Quote: ... Elution was performed with imidazole and the second step of purification was performed using Superose 6 Increase 10/300 GL gel filtration chromatography (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... 2% glycerol and 250 mM imidazole and further purified on a Superdex 200 HiLoad 16/600 column (GE Healthcare) in the gel filtration buffer (30 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... 300 mM imidazole) using AKTAprime plus (GE Healthcare). IN or CCD fractions were concentrated using Amicon Ultra-30K or -10K device (Merck Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... 40 mM imidazole and HiTrap Excel (GE healthcare) was used for BdLYR2 that was eluted in 50 mM Na phosphate pH8 ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a nickel column (TBS 0.2% LDAO 25mM imidazole) and the eluted sample (250 mM imidazole) further purified by size exclusion chromatography (HiLoad 26/600 Superdex 200 GE healthcare) using 10 mM HEPES pH 7.5 ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Fractions were eluted with an imidazole buffer (50 mM NaH2PO4, pH 7.4, 300 mM NaCl, 500 mM imidazole) on an AKTA Prime Plus (GE Healthcare, Piscataway, NJ). Fractions were analyzed by SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Genetics 2019Quote: ... Asci were stained with DAPI (4’,6-diamidino-2-phenylindole) and visualized by the DeltaVision Core Imaging System (Applied Precision, LLC, USA).
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Neuroscience 2021Quote: ... The affinity tag for Munc18 was cleaved by 2 mg of TEV in PBS with 400 mM imidazole overnight at 4℃ and additional purification was performed using superdex 200 10/300 GL (GE Healthcare, Piscataway, NJ) gel filtration column in TBS buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... 50 mM imidazole) before eluting the protein (HBS, 250 mM imidazole) following desalting using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium) to remove imidazole ...
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bioRxiv - Immunology 2022Quote: ... The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium). The Avi-His6-tag was cleaved by caspase-3 (produced in-house ...
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bioRxiv - Biophysics 2021Quote: ... imidazole was removed using a PD10 desalting column (GE Healthcare) equilibrated with Buffer A1 for Na+ or Buffer A2 for K+ ...
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bioRxiv - Microbiology 2021Quote: ... imidazole was removed using a PD-minitrap column (GE Healthcare) for buffer exchange to AB.
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bioRxiv - Microbiology 2020Quote: ... The Imidazole was eliminated using the PD10 columns (GE Healthcare). The proteins were concentrated using Vivaspin columns (SIGMA ...
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bioRxiv - Immunology 2021Quote: ... 2-D and 3-D images were obtained and analyzed using the software program MicroView (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... Imidazole was removed using PD MiniTrap G-25 column (GE Healthcare). The protein was then treated with His-tagged PreScission protease to remove the C-terminal 8 × His tag at 4 °C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Imidazole was then removed using a PD-10 column (GE Healthcare). The concentrations of sybody preparations were determined by measuring A280 and the quality of the purified sybodies was assessed by SDS-PAGE and SEC.
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bioRxiv - Biophysics 2020Quote: ... The imidazole was removed using PD-10 desalting columns (GE HealthCare) or 24 hours of dialysis using SnakeSkin dialysis tubing (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM Imidazole using a Desalting 26/10 column (GE Healthcare). The His-tag was then removed by incubation with the tobacco etch virus (TEV ...
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bioRxiv - Bioengineering 2019Quote: ... cells debris were removed by centrifugation at 16,000 × g for 20 min at 4 °C and the supernatant (supplemented with 20 mM Imidazole) was loaded on a 1 mL Ni+-HisTrapHP column (GE Healthcare #71-5027-68 AK) and washed with a buffer containing 100 mM Tris-HCl (pH 7) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We used the 2-D Quant Kit (GE Healthcare, Uppsala, Sweden) to determine protein concentrations.
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bioRxiv - Immunology 2023Quote: ... Contaminants were removed with 2-D Clean-up kit (GE Healthcare), and in-tube trypsin digestion was performed ...
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bioRxiv - Microbiology 2020Quote: ... 500 mM imidazole) via an AKTA pure system (GE Healthcare Life Sciences). Peak fractions were pooled and diluted in buffer A to a final imidazole concentration < 100 mM ...
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bioRxiv - Microbiology 2020Quote: Imidazole was removed using a PD-10 Desalting Column (GE Healthcare, UK) with an imidazole-free equilibration buffer (50 mM Tris/HCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... imidazole desalting was performed using PD-10 desalting columns (GE Healthcare #17085101) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1H were collected using a Deltavision Elite system (GE Healthcare) controlling a Scientific CMOS camera (pco.edge 5.5).
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the imidazole was removed using a PD-10 desalting column (GE Healthcare, USA).
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bioRxiv - Molecular Biology 2019Quote: ... +40 mM imidazole for washing) using an ÄKTA Purifier 10 system (GE Healthcare). The proteins were eluted by a 15 mL gradient from 40 mM to 300 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Excessive imidazole was removed using a Sephadex XK 26/60 column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... The imidazole was removed using a PD-10 desalting column (GE Healthcare, USA). The following protein-specific modifications were applied to the above protocol ...
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bioRxiv - Biophysics 2023Quote: ... The imidazole was removed by desalting column (PD MiniTrap G-25, GE Healthcare). Final protein concentration was determined by A280 using an extinction coefficient of 75000 M-1cm-1 (ACKR3 ...