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Citations for GE Life Sciences :
1 - 50 of 1706 citations for 5 SULFOPICOLINIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Molecular Biology 2021Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Crude extract was then prepared and subjected to purification by nickel-nitrilotriacetic acid (Ni2+−NTA) metal affinity chromatography using a 5 ml HisTrap™ HP column (29-0510-21; GE Healthcare). MenI was eluted at 200 mM imidazole ...
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... Nickelnitrilotriacetic acid-agarose was from GE Healthcare. Deidentified ...
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bioRxiv - Immunology 2021Quote: ... and 1X non-essential amino acids (HyClone, GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... and 1X non-essential amino acids (HyClone, GE Healthcare). T cells were activated for 2 days with anti-CD3/CD28 magnetic dynabeads (Thermo Fisher ...
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bioRxiv - Pathology 2019Quote: ... 5ug/mL Polyadenylic acid (GE Healthcare 27-4110-01), 2.5ug/mL pd (t)12-18 (Oligo dT ...
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bioRxiv - Pathology 2023Quote: ... packed with Ni2+ nitrilotriacetic acid (NTA)-sepharose (GE Healthcare) were used ...
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bioRxiv - Microbiology 2023Quote: ... 17µl (∼ 5 µg) of each Cy-5-labelled (GE Healthcare) cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare ...
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bioRxiv - Physiology 2020Quote: ... non-essential amino acids (GE Healthcare HyClone Laboratories, Logan, UT), 1X Penicillin/Streptomycin and Gentamicin (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Biochemistry 2020Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was purchased from GE Healthcare. The plasmid encoded an N-terminal 8X His-SUMO-tagged hNatD in the pET-21a vector was obtained from Genscript ...
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bioRxiv - Cell Biology 2021Quote: ... FBS and MEM Non-essential amino acids (NEAA) from GE Healthcare Life Sciences (Logan ...
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bioRxiv - Cell Biology 2022Quote: ... and 1x non-essential amino acids (GE Healthcare, Pittsburgh, PA, USA). 3T3-L1 cell lines with PTRF/Cavin-1 inactivated by CRISPR (K5 and K3 ...
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bioRxiv - Immunology 2019Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0–0.5 M NaCl in 20 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Immunology 2021Quote: ... and proteins purified from culture supernatants Ni-nitriloacetic acid (NTA) resin (GE HealthCare).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The recombinant protein was purified through a nickel-nitrilotriacetic acid column (GE Healthcare) and subsequently loaded on a gel filtration column Sephacryl S-200 HR (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluate was further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were pooled and protein purity was assessed by SDS–PAGE and Coomassie staining ...
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bioRxiv - Biophysics 2021Quote: ... followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare). Purified Nap1 was snap frozen in buffer containing 10 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... packed in water in a 5 mm inner diameter Tricon 5/100 Column (GE Healthcare, USA). Packing occurred by combined sedimentation and a steady flow of 100 µl/min through the column ...
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bioRxiv - Biochemistry 2019Quote: ... 5% (vol/vol) glycerol and 5 mM β-mercaptoethanol using a HiTrap Desalting column (GE Healthcare) and the GST tag was cleaved with GST-TEV protease overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with Ni-nitrilotriacetic acid (NTA) affinity chromatography (HisTrap HP columns from GE Healthcare) in an AKTA purifier (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Overnight trichloroacetic acid/acetone precipitation was performed with 2D Clean-Up kits (GE Healthcare) as instructed by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... MBNL1 (amino acids 1-269 of MBNL140) was obtained in pGEX-6P1 (GE Healthcare). Plasmids were transformed into E ...
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bioRxiv - Immunology 2020Quote: ... The protein was first purified with a Ni2+-nitrilotriacetic acid (NTA) resin (GE Healthcare,) using an elution buffer consisting of 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... a modified trichloroacetic acid–acetone precipitation method (Clean-Up Kit; GE Healthcare, Munich, Germany) was used ...
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bioRxiv - Microbiology 2023Quote: ... Radioactively labelled proteins or nucleic acids were visualised using storage phosphor screens (GE Healthcare) and a Typhoon 9400 imager (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... GSTrap columns (5 mL; GE Healthcare) were used with an Akta Prime FPLC system (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... 5 ml of HisTrap (GE Healthcare) was used for the affinity purification and Gel filtration was performed on HiLoad 16/60 Superdex 200 (GE Healthcare) ...
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bioRxiv - Physiology 2022Quote: ... including 5% serum (Hyclone, GE Healthcare), 1% penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... the H3 and H4 monomers were purified using a mono-Q HR 5/5 column (GE healthcare) and SP ion exchange resin (GE Healthcare) ...