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Citations for GE Life Sciences :
301 - 350 of 1803 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... the protein mixture was loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer B ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Plant Biology 2020Quote: ... Image was acquired with Image Quant LAS 4000 (GE Healthcare, IL). The band intensity was quantified with ImageJ (NIH).
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bioRxiv - Plant Biology 2019Quote: ... were precipitated using the 2D Cleanup kit (GE Healthcare, Chicago, IL), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... amplicons were purified using illustra ExoProStar (GE Healthcare, Chicago IL, USA) and sequenced using the BigDye Terminator Cycle Sequencing Kit v 3.1 (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was performed using ECL Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... and anion exchange column HiTrap Q HP (GE Healthcare, IL, USA). All tags for purification were cut and removed ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Membrane was sequentially probed overnight with primary antibodies (see Supplementary Table 6 ...
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bioRxiv - Neuroscience 2019Quote: ... 10% fetal bovine serum (FBS; GE Healthcare Life Sciences, Chicago, IL), 5% horse serum (HS ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 5% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL), 2 mM L-glutamine (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Following blocking ...
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bioRxiv - Cell Biology 2021Quote: ... followed by chemiluminescent ECL detection (Amersham, GE Healthcare, Chicago, IL, USA).
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bioRxiv - Plant Biology 2022Quote: ... and a desalting column (PD-10, GE Healthcare, Chicago, IL, USA) was used for removing sucrose and for buffer exchange ...
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bioRxiv - Microbiology 2022Quote: ... following centrifugation in Ficoll-Paque Centrifugation Media (GE Healthcare, Chicago, IL) for 25 minutes at 500 × g.
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated using Ficoll-paque plus (GE Healthcare, Chicago, IL). CD8+ T cells were purified using CD8+ Isolation Kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 5% fetal bovine serum (HyClone, GE Healthcare, Chicago, IL) and 10 μg/ml gentamicin (MilliporeSigma ...
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bioRxiv - Cell Biology 2022Quote: ... purified using an S-400 spin column (GE Healthcare, IL, USA), ligated ...
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bioRxiv - Microbiology 2021Quote: ... using a HiTrap Chelating HP column (GE Healthcare, Chicago, IL, USA) loaded with Zn2+ ions and equilibrated with low-histidine buffer ...
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bioRxiv - Cell Biology 2019Quote: ... an IN Cell Analyzer 2000 automated imager (GE Healthcare, Chicago, IL), was used for sample imaging.
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bioRxiv - Pathology 2020Quote: ... The Encore v.13 2011 (GE Healthcare Systems, Chicago, IL, USA) software system was used for data analysis ...
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bioRxiv - Neuroscience 2019Quote: ... Images were acquired automatically using an InCell2200 (GE Healthcare, Chicago, IL) or a Cell Insight CX5 (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... ECL Prime Western Blotting Detection Reagent (GE Healthcare; Chicago, IL, USA) was used to develop the membrane ...
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bioRxiv - Cell Biology 2019Quote: ... HisTRAP FF and DEAE columns were from GE Healthcare (Chicago, IL).
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant protein was purified using HiTrap DEAEFF (GE Healthcare, Chicago IL), HisTrapHP (Ni-NTA ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was done with ECL Substrate (GE Healthcare, Chicago, IL, USA) and X-ray films.
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bioRxiv - Cell Biology 2021Quote: ... signals were visualized using ECL Prime (GE Healthcare, Chicago, IL, USA) and an image analyzer 680 (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The homogenates were subjected to Percoll (GE Healthcare, Chicago, IL, USA) density gradient centrifugation with four layers (12% ...
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bioRxiv - Physiology 2022Quote: ... and acquired using ImageQuant LAS 4000 (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl using PD10 columns (GE Healthcare, Chicago, IL, USA). Finally ...
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bioRxiv - Plant Biology 2022Quote: Detection was achieved using ECL (Amersham, GE Healthcare, Chicago, IL, USA). The antibodies used were α-FLAG (F1804 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA HisTrap columns (GE Healthcare, Chicago, IL, USA) were used ...
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bioRxiv - Neuroscience 2024Quote: ... and imaged using an Amersham imager 600 (GE Healthcare, Chicago, IL). Membranes were stripped using Restore™ PLUS Western Blot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... software (Artificial-Intelligent Radio-Genomics Kits; GE Healthcare, Chicago, IL, USA) used in [1] is not publicly available ...
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bioRxiv - Cancer Biology 2023Quote: ... Chemiluminescence was detected using ImageQuant™ LAS4000 (GE Healthcare, Chicago, IL).
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bioRxiv - Neuroscience 2023Quote: ... was inserted under real-time ultrasound (GE Healthcare, Chicago, IL, USA) guidance into the radial nerve proximal to the elbow or the superficial peroneal nerve proximal to the ankle ...
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bioRxiv - Immunology 2023Quote: ... 1 mL pre-packed HisTrap HP column (GE Healthcare, IL, USA). The column was washed with 15 mL of binding buffer (20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2024Quote: ... Incubation with HRP-conjugated secondary antibodies (GE Healthcare, Chicago, IL, USA) was performed at room temperature for 1 h (dilution 1:10 000) ...
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bioRxiv - Biophysics 2020Quote: Hsc70 and MTB-Hsp70 were purified first by Ni-NTA column chromatography (His-trap, GE Healthcare). The column was washed with 50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... The excessive imidazole was removed using a desalting column (Hi-Prep™ 26/10, GE Healthcare). The 8 × His-SUMO was cleaved by TEV proteinase and the cleaved fragment was removed through the affinity of the His-tag to a Ni-NTA column ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Cell Biology 2021Quote: ... His⨯6-tagged proteins in the supernatant were purified by Ni-NTA agarose beads (GE Healthcare). After extensive wash by a buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a heparin column (Hi-trap Heparin HP, GE Healthcare Life Sciences) and MatP was eluted with a NaCl gradient (0.3 to 1M ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified from supernatants on HisTrap HP columns using an ÄKTApure (GE Healthcare) and resolved by gel filtration on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared via centrifugation and loaded to His Spin Trap spin column (GE Healthcare). The column was sequentially washed with PBC containing 20mM ...