Labshake search
Citations for GE Life Sciences :
2551 - 2600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... followed by protein G Sepharose beads (GE healthcare life science, Cat #17061801) for 2 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was vacuum-transferred to Hybond-XL (GE Healthcare) as described previously (Sasaki and Kobayashi ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and quantified using ImageQuant TL software (GE Healthcare Life Sciences). Band density was normalized to total protein ...
-
bioRxiv - Cell Biology 2023Quote: ... G-protein coupled sepharose beads (GE Healthcare) were pre-washed in lysis buffer and blocked overnight in the lysis buffer with 1% BSA ...
-
bioRxiv - Cell Biology 2023Quote: ... then electrotransferred onto Hybond-P membrane (GE Healthcare), immunodetected using appropriate primary and secondary antibodies (conjugated with HRP ...
-
bioRxiv - Biochemistry 2023Quote: ... The flow through (containing soluble free histones) was filtered with 0.22 μ filters and loaded on a cation exchange Resource S column (GE Healthcare) equilibrated with the dilution buffer EB5_1 (50 mM Tris-HCl pH8) ...
-
bioRxiv - Microbiology 2023Quote: ... The proteins were bound to IgG Sepharose 6 Fast-Flow (GE Healthcare) via the ZZ tag on the heavy chain ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes are then incubated with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosci-ences) and luminal signal was visualized and imaged in Fusion FX7 image and analytics system (Vilber Lourmat).
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were transferred to PVDF membranes (GE Healthcare), blocked in 5% non-fat milk (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Gels were then dried at 80°C for 45 min and exposed overnight to a storage phosphor screen (GE Healthcare, BAS-IP SR 2025), which was subsequently imaged with Typhoon FLA700 gel scanner and quantified in Fiji/ImageJ.
-
bioRxiv - Cell Biology 2023Quote: ... as well as 10% Hyclone™ III FetalClone™ bovine serum (GE Healthcare). When plated for experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... The cleaved protein was then separated from tag and TEV by a second Ni-NTA affinity column and further purified using size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) in 50 mM Tris ...
-
bioRxiv - Bioengineering 2023Quote: ... and the ends of the image were cropped flat to a length of 17 mm (MicroView ABA 2.1.1., GE Healthcare, London, Ontario, Canada). Past work has suggested that the use of a global segmentation threshold for a cohort with a broad range of bone volume fraction or varying bone mineral density can result in an inaccurate topology (Bouxsein et al. ...
-
bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
-
bioRxiv - Genomics 2023Quote: ... 50 ml of sea water was filtered through a 1.2 μm pore-size acrodisc (FP 30/1.2 CA-S cat N° 10462260 Whatman GE Healthcare Sciences) and used to inoculate culture flasks with 10 ml of filtrate each ...
-
bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare) and stored in 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: Glutathione Sepharose 4B beads (GE Healthcare) were used to bind GST-tagged bait proteins ...
-
bioRxiv - Systems Biology 2023Quote: ... followed by DTT removal using a PD-10 desalting column with Sephadex G-25 resin (from GE healthcare). The reduced protein was mixed with a ten fold excess of the nitroxide spin label MTSL and incubated at room temperature protected from light for one hour ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmid pGEX-4T1 was purchased from GE Healthcare (Cat #: 28-9545-49). Plasmid pET His6 Sumo TEV LIC (Addgene # 29659 ...
-
bioRxiv - Immunology 2023Quote: PBMCs were isolated from the blood of healthy volunteers using Ficoll-Plaque (GE Healthcare) density centrifugation according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). Fractions containing pure Ras protein were pooled and concentrated to 20 mg/mL and stored at –78 ºC ...
-
bioRxiv - Immunology 2023Quote: ... Lymphocytes were further enriched by centrifugation at room temperature at 695 g for 8 min in 37.5% Percoll (GE Healthcare), prior to washing and resuspension for downstream analysis.
-
bioRxiv - Microbiology 2023Quote: ... The eluant was concentrated and further purified using a Superdex-200 (GE Healthcare) column equilibrated with a buffer containing 10 mM Tris–HCl pH 8.0 ...
-
bioRxiv - Microbiology 2023Quote: ... Kinetic rate constants were extracted from this curve fit using Biacore evaluation software (GE healthcare).
-
bioRxiv - Microbiology 2023Quote: ... 10 mM NaCl and 2 mM DTT by a desalting column (GE Healthcare), and was further purified by ion exchange chromatography with Resource Q column (GE Healthcare) ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (1×PBS ...
-
bioRxiv - Microbiology 2023Quote: ... The eluant of protein was concentrated and further purified using a Superdex-200 increase 10/300 GL (GE Healthcare) column equilibrated with a buffer containing 10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2023Quote: ... VLP genomic DNA underwent amplification using the Illustra GenomiPhi V2 DNA Amplification Kit (formerly GE Healthcare Life Sciences but now Cytiva ...
-
bioRxiv - Microbiology 2023Quote: ... The relevant oligonucleotide of SV40 large T antigen NLS (PPKKKRKVED) was ligated into the pGEX2T vector (GE Healthcare) carrying the GFP gene at the C-terminus of the multi-cloning site to produce GST-SV40NLS-GFP protein (referred to as SV40 NLS) ...
-
bioRxiv - Microbiology 2023Quote: ... Importin-α and Ran proteins lacking GST were prepared by cleavage with PreScission protease (20 U/mg of fusion protein; GE Healthcare, Uppsala, Sweden) at 4 °C for 12 h ...
-
bioRxiv - Molecular Biology 2023Quote: Cell lysates were mixed with 400 µl IgG SepharoseTM 6 Fast Flow slurry (GE Healthcare) pre-equilibrated with TNM100 buffer (50 mM Tris-HCl pH 7.8 ...
-
bioRxiv - Microbiology 2023Quote: ... This suspension was slowly added to the top of 4 mLFicoll-Paque density gradient (GE Healthcare #17-1440-02, Uppsala, Sweden) and centrifuged for 40 minutes at 400 x g ...
-
bioRxiv - Microbiology 2023Quote: ... Unencapsulated ANTS and DPX were removed by gel filtration through a 5 mL HiTrap Desalting Sephadex G-25 resin column (GE Healthcare Life Sciences). To ensure the correct size and obtain LUVs ...
-
bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
-
bioRxiv - Microbiology 2023Quote: ... then passed over a size exclusion column (HiLoad Superdex 200 PG, GE Life Sciences) in a buffer containing 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... Imaging was performed with a DeltaVision Elite Deconvolution microscope (Applied Precision, Issaquah, WA, USA). Images were further processed by the aggressive deconvolution algorithm in DeltaVision SoftWoRx 6.5.2 Image Analysis Program ...
-
bioRxiv - Microbiology 2023Quote: ... the viral lysate was filtered through a 1.2 µm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 µm pore size Nalgene Rapid-Flow filter unit (PES ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting supernatant was applied to a 10-mL column with 1 mL of Ni-Sepharose (GE Healthcare Life Sciences, 17531801) equilibrated with binding buffer (20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2023Quote: ... The reaction products were analyzed by a denaturing 20% acrylamide gel and imaged with a Typhoon Imager (GE Healthcare).
-
bioRxiv - Microbiology 2023Quote: ... ECL™ Prime Western Blotting Detection Reagent (GE Healthcare) used as a substrate.
-
bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
-
bioRxiv - Microbiology 2023Quote: ... This step was followed by size-exclusion chromatography using Superdex 200 16/600 column (GE Healthcare). The proteins were concentrated by centrifugal filters (Amicon Ultra 10K ...
-
bioRxiv - Microbiology 2023Quote: ... Images were acquired on an ImageQuant LAS 500 (GE Healthcare) and analyzed with Fiji software.
-
bioRxiv - Molecular Biology 2023Quote: ... were incubated for 10 minutes on ice before subjecting them to analytical size exclusion chromatography using a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in a buffer composed of 20 mM HEPES- KOH pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... A Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) was pre-equilibrated with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubated with HRP-conjugated secondary antibodies (1:5000, GE Healthcare, USA). Protein bands were visualized using an ECL kit (Ez WestLumi plus ...
-
bioRxiv - Immunology 2023Quote: ... The resulting pellet was then resuspended in 4ml of 37.5% Percoll (GE healthcare), diluted in PBS+BSA from osmotically normalised stock and centrifuged (1800rpm ...
-
bioRxiv - Microbiology 2023Quote: ... Cultures were collected on Whatman GF/C filters with 1.2 μm pore size (GE Healthcare, US) under vacuum (750 mbar) ...