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Citations for GE Life Sciences :
151 - 200 of 1384 citations for IL 23 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for GST-tagged proteins at 4 °C following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were passed over a 1 ml His-Trap HF column (GE Healthcare). The bound protein was washed with 15 ml high salt wash buffer (20 mM potassium HEPES ...
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bioRxiv - Biochemistry 2021Quote: IMAC purification was performed using a His-Trap HP 1 ml (GE Healthcare) on an ÄKTA START protein purification system (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was purified by His Trap™ FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and applied to a 1-ml chelating Hi-Trap Sepharose column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2019Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... RNase HI was loaded onto a 16/600 Superdex 75 column (GE Healthcare), whilst PolI and Ligase were further purified on a 16/600 Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a 3ml His SpinTrap™ column (GE Healthcare) previously charged with Ni2+ and equilibrated in lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Immunology 2020Quote: ... Harvested supernatants were applied to a Hi-Trap Nickel excel columns (GE Healthcare) using an Akta pure (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was loaded onto a nickel-chelated Hi-trap column (GE Healthcare) and eluted with a linear gradient of 25-500 mM imidazole in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-p300 was further purified on a Q sepharose column (HiTrap, GE Healthcare) in BC buffer and eluted between 100-600 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-his was purified using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Nbs from PE were purified using IMAC Hi-Trap columns (GE Healthcare, 17092003) following manufacturer’s instructions and eluted using 300 nM Imidazole ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... All the His-tagged proteins were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100 mM-500 mM Imidazole gradients ...
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bioRxiv - Microbiology 2023Quote: ... The F341_RBP was purified using Ni2+- NTA-affinity chromatography (His GraviTrap, GE Healthcare) according to manufacturer’s instructions and exchanged into PBS buffer using 10 kDA Amicon® Ultra-15 Centrifugal filter units ...
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bioRxiv - Cell Biology 2021Quote: ... 5% fetal bovine serum (FBS; GE Healthcare, Chicago, IL), and 0.1% (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... and detected with ECL Prime (GE Healthcare, Chicago, IL) and a CCD camera (G:BOX Chemi XT4 ...
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bioRxiv - Biochemistry 2020Quote: ... 1ml Sephadex G-50 (GE Healthcare Life Sciences, IL), which was equilibrated with 50 mM Tris-HCl (pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins transferred onto nitrocellulose (GE Healthcare, Chicago, IL) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2021Quote: ... transferred to hybond PVDF membrane (GE Healthcare, Chicago, IL), and incubated with the following antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... using a HiTrap Desalting column (GE Healthcare, Chicago, IL). Labeled lipoproteins were collected in the void volume.
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bioRxiv - Biophysics 2019Quote: ... pore size 0.45 μm (GE Healthcare, Chicago IL, USA). Membrane was blocked with 5% fat free milk in TBS and incubate overnight at 4°C with Vimentin antibody (dilution 1:1000 in BSA 5% ...
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bioRxiv - Synthetic Biology 2020Quote: 50 nmol of Cy5-maleimide (GE Healthcare, Chicago, IL) dissolved in anhydrous DMSO (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... followed by ECL prime (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Immunology 2020Quote: ... Ficoll was purchased from GE healthcare (Chicago, IL, USA), ultrapure water ...
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bioRxiv - Microbiology 2021Quote: ... HRP-conjugated anti-mouse (NA931, GE Healthcare, IL USA), anti-rabbit (NA934 ...
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bioRxiv - Biophysics 2022Quote: ... a PD-10 desalting column (GE Healthcare, Chicago, IL) was used to remove excess imidazole and buffer exchange in between the Ni affinity purification and thrombin cleavage ...
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bioRxiv - Biochemistry 2023Quote: ... on an Äkta explorer FPLC (GE Healthcare, Chicago, IL). The column was washed with 12 column volumes (CV ...
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bioRxiv - Pathology 2023Quote: ... purified by 30% Percoll (GE Healthcare, Chicago, IL; 17089101) density gradient separation ...
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bioRxiv - Microbiology 2023Quote: ... 90V onto nitrocellulose membranes (GE Healthcare, Chicago, IL USA). The blocked membranes were probed with either rabbit polyclonal MMTV anti-Gag antibody (Rockland Immunochemicals ...
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bioRxiv - Cell Biology 2023Quote: ... Glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL, USA); and reduced glutathione (Fujifilm) ...
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bioRxiv - Biochemistry 2023Quote: ... Superdex 200 pg column (GE Healthcare, Chicago, IL, USA) using acylase buffer without glycerol.
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bioRxiv - Cell Biology 2023Quote: ... using an Amersham Imager 600 (GE Healthcare, Chicago, IL) imaging system ...
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bioRxiv - Biochemistry 2024Quote: ... Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) was then used to capture the 6×His-GB1 and 6×His-TEV ...
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bioRxiv - Cell Biology 2023Quote: GST and GST-DysSR22-23 were produced in BL21 bacteria using a standard protocol and were purified on Glutathione Sepharose 4B (GE Healthcare) [55] ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Bands were detected using enhanced chemiluminescence (Pierce Biotechnology, IL, USA) and imaged with a LAS 400 Mini Imaging System (GE Healthcare, IL, USA). Immuno-positive bands were quantified using ImageJ software (NIH ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...
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bioRxiv - Molecular Biology 2021Quote: ... using a Hi-Load 16/600 Superdex 200 prep grade column (GE Healthcare Bioscience) equilibrated with 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... tNPR1-His proteins were further purified by HiLoad 16/600 Superdex 200 (GE Healthcare) with 200 mM NaHCO3 ...
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bioRxiv - Microbiology 2020Quote: ... and supernatants loaded onto 5 mL HP HIS-TRAP columns (GE Healthcare Life Sciences) equilibrated in buffer A (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...