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Citations for GE Life Sciences :
1601 - 1650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... the excess unreacted dye was removed from each protein by passing it through a 600 μl Sephadex G-25 Fine resin (GE Healthcare), pre-equilibrated with a buffer containing 20 mM Hepes-K pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein fractions were run through a desalting column (PD-10, GE Healthcare), the buffer was exchanged into 3 mL ion-exchange Buffer A (50 mM Bis-tris pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The flow through was collected and further purified by size exclusion chromatography on the Superdex 200 Increase 10/300 GL (GE Healthcare). Peak proteins were pooled and verified by denaturing SDS PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... using 10/12% acrylamide/bisacrylamide (29:1) 0.1% SDS gels and transferred to a 0.2 μm PVDF Hybond-P membranes (Amersham/GE healthcare) at 20V for 1h in a semi-dry Trans-Blot SD cell system (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... 40 µL of the mixture was injected into a Superdex 75 10/300 GL gel filtration column (GE Healthcare) pre-equilibrated with the Rab buffer with a flow rate of 0.5 mL/min at room temperature and absorption at 280 nm was recorded.
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bioRxiv - Bioengineering 2024Quote: ... The column was attached to an ÄKTA Pure FPLC (GE Healthcare Biosciences, Sweden) equipped with a P960 sample pump and analyzed with UNICORN 5.0 software (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... GST-Ubl Parkin was purified using Glutathione Sepharose™ 4B (GE Healthcare #17-0756-01), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... ImageQuant TL (GE Healthcare, software v8.1) densitometry analysis was used to determine total protein and band intensity ...
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bioRxiv - Microbiology 2024Quote: ... The filtrate was loaded onto a HiPrep Sephacryl S-400 HR 16-600 column (GE Healthcare), which had been equilibrated with filtered mi3 buffer using an ÄKTA Pure 25 system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... The signal was amplified with ECL substrate (GE Healthcare, RPN2106) and detected in Image Quant LAS4000 using a CCD camera.
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bioRxiv - Microbiology 2024Quote: ... separated by SDS-PAGE on 8% acrylamide gels and transferred onto nitrocellulose membrane (GE healthcare). Membranes were stained with Revert™ 700 total protein stain (LI-COR ...
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bioRxiv - Neuroscience 2024Quote: ... The fragment was inserted into a pCAGGS vector (GE Healthcare, Chicago, IL). shRNA vectors were generated by inserting double-stranded oligonucleotides into the mU6 pro vector ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5 mL of Streptavidin-Sepharose beads (GE Healthcare) were first added to a mounted sintered Buchner funnel (<10 μm pore size) ...
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bioRxiv - Biochemistry 2024Quote: ... Debris-free supernatant was loaded onto Ni-NTA FF-HisTrap columns (GE Healthcare) for affinity purification via the hexahistidine tag ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with ECL Western blotting reagent (GE Healthcare), images were acquired with the ChemiDoc MP System (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was subjected to Ni-immobilized metal affinity chromatography (IMAC) (HisTrap FF Crude column, 5 ml, GE Healthcare). Proteins were eluted with a linear gradient of imidazole (10-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... and loaded onto a Superdex 75 26/60 column (GE Healthcare) equilibrated with 20 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... The analysis of the spots intensities and background noise removal was performed using ImageQuant TL software (Version 8.1, GE Healthcare). Each spot intensity was quantified as duplicates and the mean calculated ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved sample was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences), and the flowthrough was collected ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered soluble cell solution was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences) in Lysis Buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and the HiLoad 26/600 Superdex 200 column (GE Healthcare Life Sciences) was equilibrated in size-exclusion buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Antibodies were separated from unincorporated fluorophores on desalting columns (NAP-5 or PD-10, GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... and further purified with a size-exclusion chromatography (SEC) column (Superdex 75 HiLoad26/260, GE Healthcare) by using 50 mM Tris-HCl pH 7.5 as the running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... this was done by size exclusion chromatography using either a Superdex200 Increase column (GE Life Sciences) [150-300 mM NaCl and 50 mM Tris-HCl buffer (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... the reaction mix was loaded onto an Superdex 75 Increase column (GE Life Sciences) and separated using a buffer containing 50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... equilibrated with the same buffer on an AKTA-micro system (GE Healthcare). Elution of proteins was monitored at 280 nm ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were applied onto a Superose 12 10/300 GL column (GE Healthcare), which had been pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2024Quote: ... The flow through of this column was concentrated and purified by SEC (Superdex 75 HiLoad26/260, GE Healthcare) using NMR buffer (20 mM HEPES pH 7.3 ...
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bioRxiv - Biochemistry 2024Quote: ... and loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with SPNHC-C8E4 buffer (100 mM sodium chloride ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-rat antibody (GE Healthcare). Proteins were detected by enhanced chemiluminescence (ECL western blotting detection system ...
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bioRxiv - Plant Biology 2024Quote: ... Labelled probes were separated from unincorporated γ32P-ATP by gel filtration using G-25 MicroSpin Columns (GE Healthcare Life Sciences). The U6 probe was end-labelled the same way.
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on SDS-PAGE and fluorescently labelled proteins were detected by in-gel scanning with the Typhoon FLA 9000 (GE Healthcare Life Sciences) using Cy3 settings (532nm excitation and 610PB filter).
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bioRxiv - Plant Biology 2024Quote: ... Detection was performed with a Typhoon FLA 9500 (GE Healthcare). Oligonucleotide used for probes are listen in Supplementary Figure 8.
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bioRxiv - Plant Biology 2024Quote: ... and visualised using the ImageQuant® LAS-4000 imager (GE Healthcare, Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... About 30 μL of equilibrated glutathione beads (17-0756-01, GE Healthcare) were incubated with the supernatant overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was separated on an 8-16% gradient Tris-glycine gels and transferred to PVDF blotting membrane (GE Healthcare Life Sciences). The membrane was immunoblotted with 1:200 anti-FGF13 antibody or 1:200 anti-pan FHF-A (Neuromab clone N235/22 ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed and incubated in Amersham Biosciences ECL Western Blotting Detection Reagent (GE Life Sciences) before exposure using a G:BOX gel doc system (Syngene) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was removed and cell pellets were re-suspended in 30% isotonic Percoll (GE Healthcare, #17089102). Cell suspensions were centrifuged for 20 min at 2000g ...
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bioRxiv - Neuroscience 2024Quote: Phosphopeptide enrichment was performed in an AKTA Purifier (GE Healthcare, Piscataway, NJ) using 5 µm titanium dioxide (TiO2 ...
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bioRxiv - Neuroscience 2024Quote: ... ECL reagent (cat no. RPN2232) and PVDF membrane (cat no. 10600023) were purchased from GE Healthcare. Anti-Cdc25A (cat no ...
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bioRxiv - Neuroscience 2024Quote: ... using the ImageQuant LAS 4000 system (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: Pooled soluble brain extracts were injected at 0.5 ml/min through a Superdex 75 (10/300) HPLC column (GE Healthcare Life Sciences, Pittsburg PA, USA) for 50 min and 0.25 ml fractions were collected ...
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bioRxiv - Neuroscience 2024Quote: ... using the Amersham ECL prime detection system (GE Healthcare Life Science, Cat. No. RPN2232) to visualize protein bands ...
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bioRxiv - Neuroscience 2024Quote: The binding kinetics of 5G2 and 1G11 to tau peptides encompassing amino acids 407-421 and 407-423 were undertaken through Biacore 2000 (GE Healthcare) using surface plasmon resonance analysis according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Signals detected using an ECL Prime Detection kit (GE Healthcare Life Sciences) and visualised with a ChemiDoc™ imaging XRS+ system (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... and detected with a 1:10000 goat anti rabbit secondary antibody conjugated with HRP and imaged with ECL (GE healthcare NA931, NA934).
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bioRxiv - Bioengineering 2024Quote: ... the product was purified with a desalting column (Sephadex G25 PD-10, GE Healthcare), and the elution fractions were concentrated with Amicon Ultra centrifugal filter units (3 kDa ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% surfactant P20 (PBS-EP) (GE Healthcare Life Sciences, Pittsburg PA, USA) containing BSA (2 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... All data were acquired on a 3T GE Discovery MR750 UHP (GE Healthcare, Milwaukee, WI, USA), equipped with a Nova 32-channel head coil.