Labshake search
Citations for GE Life Sciences :
1551 - 1600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... D199A was injected onto a Superdex 75 Increase 10/300 analytical column (GE Healthcare) equilibrated in 20 mM HEPES (pH 7.5) ...
-
bioRxiv - Biophysics 2024Quote: ... Streptactin sepharose high-performance resin (GE Healthcare) was added to the lysates and rotated for 2 hours and 30 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... The trimer was further purified by a Superdex 75 Increase 10/300 GL column (GE Healthcare), and SApNPs were characterized on a Superose 6 Increase 10/300 GL column ...
-
bioRxiv - Neuroscience 2024Quote: ... PBS was bought from GE Healthcare Life Sciences (Logan ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone).
-
bioRxiv - Microbiology 2024Quote: ... The protein was then loaded onto a HiTrap Q HP Column (GE Healthcare) and eluted using a gradient from buffer QA (50 mM Tris ...
-
bioRxiv - Immunology 2024Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0–0.5 M NaCl in 20 mM Tris (pH 8.0) ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with 20% ES cell-screened FBS (Hyclone, GE Healthcare, Chicago, USA) until colonies formed ...
-
bioRxiv - Plant Biology 2024Quote: ... The DNA bands were visualized by fluorescence imaging using a Typhoon Trio Variable Mode Imager (GE Healthcare, Chicago, IL, USA). Analyses of the binding to promoter mutants MA ...
-
bioRxiv - Plant Biology 2024Quote: ... After the proteins were purified by Glutathione Sepharose 4 Fast Flow (GE Healthcare) and Amylose Resin (New England Biolabs ...
-
bioRxiv - Cell Biology 2024Quote: ... The final purification steps were anion exchange chromatography using a QFF ANX column (GE Healthcare) and dialysis into experimental buffers ...
-
bioRxiv - Biophysics 2024Quote: ... and size-exclusion (Superose-6; GE Healthcare) chromatography.
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated with washed Ni Sepharose (High Performance, GE Healthcare) for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare) ...
-
bioRxiv - Genomics 2024Quote: ... and Cy5-dCTP (GE HealthCare) using random primer DNA labeling kit (Takara) ...
-
bioRxiv - Cell Biology 2024Quote: ... the supernatants were incubated with Glutathione Sepharose 4B (17075601; GE Healthcare) for 3 h at 4°C with gentle rotation ...
-
bioRxiv - Biochemistry 2024Quote: ... then electro-transferred on a nitrocellulose (0.45 μm pore size; GE Healthcare) membrane using 1X Bio-Rad transfer buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... developed using ECLTM Western Blotting Detection Reagents (RPN2209, GE Healthcare) and imaged using Kwikquant image analyzer and image analysis software (ver 5.2 ...
-
bioRxiv - Biochemistry 2024Quote: ... The radioactive bands were visualized in PhosphorImager (GE Healthcare) and quantitated using ImageQuant software ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then mixed with pre-washed streptavidin Sepharose beads (cat.17511301, GE Healthcare, Bronx, NY, USA) and incubated at 4°C with rotation for 3 hours ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were treated with either the ECL start reagent (GE Healthcare) or the ECL select reagent (GE Healthcare ...
-
bioRxiv - Cell Biology 2024Quote: ... or the ECL select reagent (GE Healthcare) according to the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2024Quote: ... SF650B-conjugated anti-rabbit IgG was then isolated using a Sephadex G-25 column (GE Healthcare). MRC-5 cells cultured on glass-based dishes for 2 days were subsequently incubated with 10 μg/ml anti-fibronectin IgG (F3648 ...
-
bioRxiv - Cell Biology 2024Quote: ... For western blots protein samples from 1 x 106 trypanosome cells were separated on 12.5 % sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). For protein dot blots ...
-
bioRxiv - Biochemistry 2024Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an Agilent LC-1220 system in 20 mM Mes-Tris pH 6.5 ...
-
bioRxiv - Microbiology 2024Quote: ... separated by SDS-PAGE on 8% acrylamide gels and transferred onto nitrocellulose membrane (GE healthcare). Membranes were stained with Revert™ 700 total protein stain (LI-COR ...
-
bioRxiv - Cell Biology 2024Quote: ... estimated by Bradford assays) were separated in a 10 % (w/v) polyacrylamide gel and were transferred on PVDF membranes (GE Healthcare Life Sciences Amersham). Immunodetection was performed with an anti-FLAG antibody (Agrisera ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sensograms were analyzed using BIA-T200 evaluation software version 2.1 (GE Healthcare). The relationship between protein concentration and Response unit (RU ...
-
bioRxiv - Cancer Biology 2024Quote: Surface plasmon resonance (SPR) analysis was conducted using the Biacore-T200 system (GE Healthcare) as described previously (33) ...
-
bioRxiv - Cell Biology 2024Quote: SEC-MALS was performed with a Superdex Increase S200 10/300 GL column (GE Healthcare) connected in-line with a sixteen-angle Dawn Heleos II light-scattering instrument and Optilab TrEX differential refractometer (Wyatt Technologies) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 ml protein was loaded onto the analytical size exclusion column Superdex 200 increase 10/300 GL (GE Healthcare) in running buffer (25 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2024Quote: ... Wells were washed twice with room temperature PBS and then 4 fields of view per well imaged on an InCell 6000 (GE Healthcare). Images were segmented with two custom Cellpose[45] models to segment the nucleus (from DAPI channel ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated with the ECL start reagent (GE Healthcare). Chemiluminescence images of the membranes were acquired using FUSION-SOLO.7S (Vilber-Loumat ...
-
bioRxiv - Cell Biology 2024Quote: ... Flow through the SEC-MALS system was controlled by an AKTA Pure FPLC (GE Healthcare) housed at ∼10°C ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-rabbit (GE Healthcare # NA9340; dilution 1:10,000) or anti-goat (Bio Rad #1721034 ...
-
bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
-
bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 3-h age window by isolating late schizonts from a 60% Percoll (GE Healthcare, Life Sciences) gradient and culturing these for 3 h with fresh erythrocytes (104) ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA solutions were spotted onto Hybond-N+ positively charged nylon membrane (RPN1210B, GE Healthcare). Membranes were blocked at room temperature for 1 hour in TBS-T (Tris-Buffered Saline (TBS) ...
-
bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with secondary antibodies conjugated with horseradish peroxidase (HRP, GE Healthcare Life Sciences). Immunoreactive proteins were visualized using the LumiGLO chemiluminescent substrate (Cell Signaling).
-
bioRxiv - Cancer Biology 2024Quote: ... Chromatin was precleared with blocked Protein G Sepharose beads (GE Healthcare) for 2 h at 4°C before being rotated overnight at 4°C with Anti-acetyl-Histone H3 (Lys27 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
-
bioRxiv - Developmental Biology 2024Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in CO2 independent medium (Thermo Fisher Scientific)] for 2 hours at 37°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5% FBS (Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30-60 min at 37°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... protein samples were precipitated onto Sera-Mag SpeedBeads (GE Healthcare #45152105050250 and #65152105050250) in the presence of 50% EtOH and 2.5% formic acid (FA ...
-
bioRxiv - Genetics 2024Quote: ... Whereafter the proteins were separated by SDS-PAGE and transferred onto nitrocellulose blotting membranes with a pore size of 0.45 μm (GE Healthcare, 10600002). The membranes were blocked with TBS-Tween buffer (50 mM Tris ...
-
bioRxiv - Genetics 2024Quote: ... Incubation with secondary antibodies for 1.5 hours was followed by chemiluminescence development system (GE Healthcare, Pittsburgh, USA). Antibodies details are listed in Supplemental Table 2-3.
-
bioRxiv - Genetics 2024Quote: ... before visualization using a BioRad ChemicDoc XRS+ or X-OMAT-Film processor using Hyperfilm™ ECL (GE Healthcare).
-
bioRxiv - Evolutionary Biology 2024Quote: ... Then medium was removed and replaced with 6% (wt/vol) Ficoll PM400 (GE Healthcare, cat. GE17-0300-10) in Nematostella medium ...
-
bioRxiv - Immunology 2024Quote: ... were obtained via from the Massachusetts General Hospital Blood Transfusion Service followed by density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare) as per the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2024Quote: SIM images were acquired using an OMX4 (GE Healthcare) with DeltaVision Immersion Oil 1.514 and a 60x 1.42 NA oil immersion objective (Olympus) ...
-
bioRxiv - Developmental Biology 2024Quote: ... the membrane was washed with 0.1% TBST and incubated with corresponding secondary antibody conjugated to Horse Radish Peroxidase (HRP) at 1:10000 (GE life sciences; NA934) dilutions for one hour at room temperature ...