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Citations for GE Life Sciences :
1351 - 1400 of 2823 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The solubilized fraction was subjected to affinity purification by 1D4 antibody conjugated to CNBr-activated agarose (GE Healthcare). The resin was extensively washed and the protein eluted with wash buffer supplemented with 0.2 mg/ml 1D4 peptide ...
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bioRxiv - Biochemistry 2020Quote: ... Antibodies used for ChIP experiments were purified with rProtein A Sepharose (PAS) beads (GE Healthcare, Cat#171279-01).
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bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
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bioRxiv - Neuroscience 2020Quote: ... the wells were washed five times and an anti-rabbit HRP conjugated detection antibody (GE Healthcare, Helsinki, Finland) was added at a 1:5000 dilution ...
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bioRxiv - Genetics 2019Quote: ... Antibody signals were developed using ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038-560) and bands were quantified with Gel Doc system (Bio Rad ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... specific for both primary antibodies and the signal was revealed by ECL Western Blotting Analysis System (GE Healthcare). Additional p-eIF4E ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies were visualized with ECL plus substrate (Pierce, 32132) on a Typhoon FLA9500 (GE Healthcare Life Sciences) for co-IP blots and a ChemiDoc MP Imager (Bio-rad ...
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bioRxiv - Biophysics 2020Quote: ... uncoupled dyes were separated from labelled antibodies by size exclusion chromatography (on Sephadex G25 PD10 columns GE Healthcare) followed by dialysis against PBS 1x overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies bound to the PVDF membrane were then detected using enhanced chemiluminescence reagents (GE Healthcare, NJ); the chemiluminescence was digitally imaged and analyzed with the ChemiDocTM MP imaging system and associated software (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatant using a HiTrap protein A column (Cytiva, GE Healthcare). IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... The apparent binding affinity (KD) for individual antibody was calculated using BIAcore® 3000 Evaluation Software (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with chemiluminescence (34095, Pierce) with a secondary antibody conjugated to Horseradish peroxidase (NXA931V, GE Healthcare). The chemiluminescence signal was obtained by the FUJI Medical X-ray film (4741019289 ...
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bioRxiv - Cell Biology 2024Quote: ... All blots were probed with an HRP-conjugated donkey anti-rabbit secondary antibody (catalog number NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse antibody (catalog number NXA931 ...
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bioRxiv - Neuroscience 2023Quote: ... and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare). Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were incubated with HRP-conjugated secondary anitbodies at 1:3,000 for 1 h at room temperature and bands developed using ECL Prime Western Blotting Detection Reagent (GE Healthcare). Blots were subsequently incubated with 1:20,000 HRP-conjugated β-actin (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... For further purification the protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column ...
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bioRxiv - Immunology 2020Quote: ... The equilibrium Kd was determined by fitting the data to a steady-state 1:1 binding model using Biacore X100 Evaluation software version 2.0 (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column and washed with Buffer A (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell culture media was replaced with a 1:1 mixture of 1X HBSS (SH30588.02, GE Healthcare Life Sciences, United States) with 20 mM HEPES (BP310-1 ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 50-250 μl of plasma were used for immunoglobulin purification via 1:1 mixture of proteinA/proteinG resin (GE Healthcare). Slurry was washed once with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 76 mm × 24 mm super frost glass slides were coated with a solution made of 14:1:1 ethanol:acetic-acid:PlusONE Bind-Silene (GE Healthcare; vol:vol:vol). In parallel ...
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bioRxiv - Neuroscience 2020Quote: ... 1:20,000-1:40,000),immunoreactive bands on the membranes were detected by enhanced chemiluminescence (ECL Plus; GE Healthcare Bio-Sciences). For total protein expression ...
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bioRxiv - Cell Biology 2021Quote: ... Affinity and kinetic constants were determined by fitting the corrected sensorgrams with the 1:1 Langmuir model using BIAevaluation v3.0 software (GE Healthcare). The equilibrium binding constant KD was determined by the ratio of the binding rate constants kd/ka.
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bioRxiv - Biophysics 2021Quote: ... 25 µM of purified SpyCatcher-GFP was mixed with excess purified SpyTag-SAT09 (1:1.5) and incubated at 4°C for 1 hr and loaded on a HiPrep Desalting Column (GE Healthcare) equilibrated with 10mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and the kinetic parameters were obtained by globally fitting the double-reference subtracted data to a 1:1 binding model with mass transport limitation using Biacore T200 Evaluation software v 3.1 (GE Healthcare). The dissociation rate constant (kd ...
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bioRxiv - Plant Biology 2019Quote: ... They were determined by fitting the experimental data with the 1:1 Langmuir model (BIA-evaluation software 4.1, GE Healthcare). The binding constants were calculated by two methods ...
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bioRxiv - Cell Biology 2020Quote: ... 100 µL of CENP-E2055-2608 and CENP-E2055- 2358 at 1 mg·ml-1 were run on a calibrated Superdex-200 10/300 GL Increase (GE Healthcare) size exclusion column pre-equilibrated in gel filtration buffer (described above ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Biochemistry 2022Quote: ... fits of the sensorgrams were determined using a 1:1 binding model (Langmuir) using Biacore T200 Evaluation Software 3.1 (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... and the raw data curves were fitted to a 1:1 binding model using Biacore 8K Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... was mixed with fibrinogen solution in a 7.5:1 molar ratio for 1 hour at room temperature and then filtered through a HiTrap desalting column (GE Healthcare) packed with Sephadex G-25 resin ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA and 1 mM DTT) and loaded onto a Mono Q 10/100 GL column (GE Healthcare, Inc.) equilibrated in buffer E and eluted with a 160 mL linear gradient of 0.3–0.5 M NaCl in buffer E ...
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bioRxiv - Biophysics 2023Quote: ... and the binding affinities were obtained using a 1:1 (Langmuir) binding fit model with Biacore T200 Evaluation software version 3.2 (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... using 1 ml Resource S column (GE healthcare) connected to a nano-HPLC (Ultimate 3000 ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-anti rabbit secondary (GE Healthcare; 1:5000) and HRP-anti mouse secondary (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... donkey anti-rabbit (NA934, GE Healthcare, 1:10000) and sheep anti-mouse (NA931 ...
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bioRxiv - Microbiology 2019Quote: ... using 1 ml HisTrap HP columns (GE Healthcare) followed by size exclusion chromatography (HiLoad 16/60 Superdex 200 ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Bovine Serum Albumin (BSA, GE Life Sciences) and 0.03% Triton X-100 in 0.1 M PBS before overnight incubation with primary antibodies (rabbit anti-cldn5 ...
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bioRxiv - Cell Biology 2021Quote: ... and donkey anti-rabbit (GE healthcare; 1:5000).
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bioRxiv - Cancer Biology 2022Quote: ... For BrdU staining (1:500, #RPN20AB, GE Healthcare) cells were fixed with ice-cold methanol (40 sec ...
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bioRxiv - Neuroscience 2020Quote: ... donkey anti-rabbit (#NA9340V, GE Healthcare, 1:4000). Membranes were washed after each incubation step three times for 5 min in TBS-T buffer (25 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-rabbit (NA9340; GE Healthcare; 1:25,000). Protein extraction was performed using lysis Buffer (20 mM Tris (pH 8) ...
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bioRxiv - Immunology 2020Quote: ... and 1 ml Mono S column (GE Healthcare) as described (14) ...
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bioRxiv - Cancer Biology 2019Quote: ... dilution 1:5000) were purchased from GE Healthcare.
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bioRxiv - Immunology 2021Quote: ... and 1% 100 × Penicillin-Streptomycin (GE Healthcare, #SV30010) at 37 °C / 5% CO2 ...