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Citations for GE Life Sciences :
851 - 900 of 1372 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Hexahistidine-tagged proteins in the soluble fraction were affinity purified using a nickel-charged His-trap immobilized metal affinity chromatography (IMAC) column (GE Healthcare, UK). Washing was carried out with lysis buffer and recombinant proteins were eluted with the same buffer containing 500 mM imidazole ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant was collected for purification using a 1 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... histones were further purified by tandem ion exchange chromatography using HiTrap Q and Hi Trap SP anion and cation exchange columns (GE Life Sciences). Histone-containing fractions were pooled ...
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bioRxiv - Immunology 2020Quote: ... Protein containing cell culture supernatants were harvested and clarified media was purified by Immobilized Metal Affinity Chromatography (IMAC) using His Trap™ Excel (GE Healthcare) using PBS buffer plus 15mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble fractions containing His-tagged PhoH2 or PhoH2-R339A were purified by IMAC on a HisTrap HP column (GE Healthcare, UK). The protein fractions were purified further by size exclusion chromatography ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using a 0.45 μm syringe filter and loaded onto a 5 mL Hi-Trap column (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... 35 mM NaCl and 20 mM Tris HCl pH 8.3) and then purified by ion exchange using a Hi Prep Q FF 16/10 column (GE Healthcare Life Sciences) connected to an Akta Pure system (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Biophysics 2022Quote: SPR analysis were carried out at 25.0 °C using Biacore T100 system (GE Healthcare, Chicago, IL, USA). In all protocols ...
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bioRxiv - Neuroscience 2020Quote: ... blots were visualized using the ECL western blotting detection reagent (GE Healthcare, IL, US; Cat. No. RPN2106) on an electrochemiluminescence10 instrument (Amersham Biosciences ...
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bioRxiv - Physiology 2019Quote: ... whole-blood samples (20 µL) were transferred onto Whatman FTA® microcards (GE Healthcare, Chicago, IL, USA). The samples were allowed to dry at room temperature and stored separately in plastic bags at −20°C ...
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bioRxiv - Biochemistry 2019Quote: ... Structured illumniation microscopy was performed using a Deltavision OMX-V4 Blaze (GE Healthcare, Chicago, IL, United States) setup equipped with 4 sCMOS (PCO ...
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bioRxiv - Plant Biology 2020Quote: ... and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2021Quote: ... separated from free dye by gel filtration using a PD-10 column (GE Life Sciences, Chicago, IL), and diluted to a working lipid concentration of 144 μM ...
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bioRxiv - Biophysics 2021Quote: ... The protein purification was carried out using a cation exchange HiTrap SP column (GE Healthcare, Chicago, IL) with phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Biophysics 2020Quote: ... the solution was loaded on a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... These fractions were then further purified using a Superdex 75 26/60 column (GE healthcare, IL, USA), and the fractions containing the recombinant protein were combined ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Immunology 2021Quote: ... and concentrated and further purified on a standardized Superdex 200 16/600 (GE Healthcare, Chicago, IL, USA) column running in HBS-E (10 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... the solution was loaded onto a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-ABCA4 antibody (CL2) to CNBr-activated Sepharose 4B beads (GE Healthcare Bio-Sciences, Chicago, IL, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Fluorescence visualization was done with the Typhoon™ FLA 9000 laser scanner (GE Healthcare, Chicago, IL, USA). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... the mixture containing DksA was pushed through a HiTrap SP column (GE Healthcare, Chicago, IL, United States) to bind the DksA protein and subsequently eluted with 50 mM – 1M gradient of sodium chloride ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was visualized using the ECL Select Western blotting detection reagent (GE Healthcare, Chicago, IL, USA) in a LAS-3000 detection system (Fujifilm ...
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bioRxiv - Microbiology 2021Quote: ... and regeneration was performed in 10mM Glycine HCl (pH 1.5, GE Healthcare, Chicago, IL, BR-1003-54). Binding-affinity constants (KD ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged by passing the eluate through a desalting PD-10 column (GE Healthcare, Chicago, IL), equilibrated with 50 mM HEPES/KOH ...
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bioRxiv - Cell Biology 2020Quote: ... Size exclusion chromatography was performed on Superdex™ 200 Increase 5/150 GL (GE Healthcare, Chicago, IL).
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bioRxiv - Neuroscience 2019Quote: ... the membrane was developed using an ECL Prime Western Blotting Detection Reagent (GE Healthcare; Chicago, IL, USA). The density of protein bands from three independent experiments was quantified using the Image J software (National Institutes of Health [NIH] ...
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bioRxiv - Synthetic Biology 2020Quote: ... The OD600nm was determined in technical triplicates using an Ultrospec 10 spectrophotometer (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... a glutathione-S-transferase (GST) (64) and thrombin sequence derived from pGEX-2T (GE Healthcare, Chicago, IL), and a maltose binding protein (MBP ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was subjected to size exclusion chromatography using HiLoad 16/60 Superdex 200 (GE Healthcare, IL) with buffer containing 100 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... blots were developed with chemiluminescence substrate kit (Pierce ECL Western Blotting Substrate-GE Healthcare, Chicago, IL, USA) and the signal was captured by a CCD camera (ImageQuant LAS 4000 mini® ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed with TBST and incubated with enhanced chemiluminescence (ECL; GE Healthcare; Chicago, IL, USA) and signals were detected with on film (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fast protein liquid chromatography on the ÄKTA pure system (GE Healthcare Life Sciences, Chicago, IL) using protein A as the first chromatography step and ceramic hydroxyapatite (CHT ...
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were imaged for 72h in an IN CELL Analyzer 2000 microscope (GE Healthcare, Chicago, IL, USA) equipped with temperature and CO2 controller ...
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bioRxiv - Immunology 2022Quote: ... MBs were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare, Chicago, IL) and eluted with 20 mM Tris pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The eluent was loaded into HiLoad 16/600 Superdex75™ pg column (GE Healthcare, Chicago, IL, USA) that was pre-equilibrated with buffer C [20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... and transferred to Amersham Protran premium 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA). Membranes were blocked with 5% skimmed milk (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... and quantified with a Typhoon 9400 scanner by ImageQuant Software version TL 8.1 (GE Healthcare, Chicago, IL). 3.6mg of protein per sample was reduced with 10mM Tris(2-carboxyethyl ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and cell pellets were re-suspended in 37% isotonic Percoll (GE Healthcare, IL, USA) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Proteins were analyzed on a Superdex 200 10/300 GL column from GE healthcare (Chicago, IL, USA) equilibrated using a mobile phase containing 150 mM KCl and 50 mM Tris-HCl pH 7.6 run at 0.4 ml/min ...
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bioRxiv - Pathology 2023Quote: ... immunoreactive bands were visualized using the enhanced chemiluminescence (ECL) detection reagent (GE Healthcare, Chicago, IL, USA, RPN2106) or acquired in the ImageQuant LAS 4000 device using the Immobilon Forte Western HRP substrate (WBLUF0100 ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was subsequently purified using a Superdex75 gel-filtration column (GE Healthcare, Chicago, IL, USA). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to Amersham Protran premium 0.45 µm nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA). Membranes were blocked with 5% skimmed milk (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...