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Citations for GE Life Sciences :
8801 - 8850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies for Western blotting include anti-mouse-HRP (GE Healthcare NA931, AB_772210), anti-rabbit-HRP (GE Healthcare NA934 ...
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bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
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bioRxiv - Immunology 2022Quote: ... TLC plates were analyzed by phosphor imaging (Typhoon FLA 9000, GE Healthcare). Genomic DNA (gDNA ...
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bioRxiv - Immunology 2022Quote: The affinity between H2 and A33 was measured at room temperature using a Biacore T100 system with CM5 chips (GE Healthcare) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... at a density of 0.3 x 106 cells/mL in 10 mL total volume/plate of complete culture medium (RPMI 1640 plus 10% heat-inactivated fetal bovine serum (FBS, GE Healthcare HyClone), 50 μM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... then loaded onto a HiLoad 16/60 Superdex 750 column (GE Healthcare) in 10 mM Tris at pH 7.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 µm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... high-content image stacks were analyzed using the InCarta software (GE Healthcare) producing comparative fluorescence intensity measurements ...
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bioRxiv - Cell Biology 2022Quote: ... Cat# 015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 μL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired using softWoRx v4.1.2 software (Applied Precision). Images were acquired every 5 minutes for 12 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Bio-Rad (4–15%) gradient gels were used for gel electrophoresis Proteins were blotted onto 0.2 µm nitrocellulose membranes (Amersham Protran, GE Healthcare) using the Thermo Scientific Pierce G2 Fast Blotter ...
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bioRxiv - Cell Biology 2022Quote: All shRNA’s (GIPZ Lentiviral constructs) were purchased from Dharmacon (GE Healthcare). Pol κ shRNA (RMM4532-EG27015 ...
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bioRxiv - Cell Biology 2022Quote: ... siRNAs and shRNAwere purchased from Dharmacon (GE Healthcare) and transfections were performed using Dharmafect II for siRNAs and Lipofectamine 2000 for shRNAs ...
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bioRxiv - Cell Biology 2022Quote: Samples seeded on #1.5 thickness coverslips were imaged on a DeltaVision Core light microscopy system (GE Healthcare) using a 100×/1.4-NA objective fitted to an Olympus IX-71 microscope stand ...
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bioRxiv - Cell Biology 2022Quote: ... using the software package softWoRx (GE Healthcare). Cells were imaged using a 0.2-μm interval and a total stack of 2 μm and deconvolved for presentation using softWoRx ...
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bioRxiv - Cell Biology 2022Quote: ... All Western blots were revealed using ECL (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant was mixed with 50 µl of streptavidin sepharose (Cat. No. 17-5113-01, GE healthcare), shaking at 4°C for 3 h after being washed with PBS once ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was visualized by Immunostar Zeta (Cat. No. 297-72403, Fujifilm-Wako chemical) and detected by using an Amersham Imager 600 (GE healthcare) or a WSE-6100 LuminoGraph I (ATTO Co. ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare), and the membrane was blocked with 5% non-fat dry milk for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with 0.1% Tween-PBS and analyzed by chemi-luminescence (GE Healthcare Life Science). Images were acquired and quantified using Alliance Mini HD6 system by UVITEC Ltd ...
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bioRxiv - Cancer Biology 2022Quote: ... and the peripheral blood mononuclear cells (PBMCs) isolated by Ficoll-Paque gradient (GE Healthcare, Boston, MA, USA). Informed consent was obtained in accordance with the Helsinki Declaration and the Review Board of the Dana-Farber Cancer Institute.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant obtained after centrifugation at 15,000 rpm for 30 min was purified using a HisTrap™ HP column (GE Healthcare, Chicago, Illinois). Next ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg of cell lysate was incubated with 40 μl of glutathione-Sepharose beads (GE Healthcare) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium pyruvate (HyClone, GE Healthcare Life Sciences, catalog # SH3023901), 100 µM β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies conjugated to HRP required for western blot were obtained from GE Healthcare.
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bioRxiv - Cell Biology 2022Quote: ... Prior to blotting of the DNA onto a positively charged nylon membrane (GE Healthcare Life Sciences), the DNA in the gel was denaturated by incubation in 0.25 M HCL solution for 5min followed by 2x/15 min in denaturation solution and 2x/15 min in neutralization solution ...
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bioRxiv - Cancer Biology 2022Quote: ... dead cells were eliminated using a Ficoll-Pague gradient (GE Healthcare, Boston, MA, USA) and live cells were allowed to recover for 24 h.
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bioRxiv - Cancer Biology 2022Quote: The SuperoseTM 6 10/300 GL gel filtration column (GE Healthcare) was equilibrated with one column volume of running buffer (20mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... on an ImageQuant LAS 4000 machine (GE Healthcare). Densitometric analysis of product bands was performed using the Gel Analyzer function in Fiji from NIH (47).
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bioRxiv - Cancer Biology 2022Quote: ... and imaged with ImageQuant LAS4000 (GE Healthcare BioScience). The intensity of the bands was assessed using ImageJ (NIH ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were developed by ECL reagents (GE Healthcare) and then exposed to X-ray film (AGFA).
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bioRxiv - Cell Biology 2022Quote: ... cells were spotted onto slides and images were acquired using a Deltavision Elite microscope (GE Healthcare Biosciences) with a 60X oil immersion objective ...
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bioRxiv - Cancer Biology 2022Quote: ... The BCA assay kit was used to quantify the isolated protein samples (GE Healthcare). The proteins were then heat-denatured and resolved on SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... and chemiluminescence scanning with ImageQuant LAS4000 (GE Healthcare). See Supplementary Table 7 for antibodies used ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently washed with 1 x TBS/T for 30 min in 10 min intervals prior to incubation in ECLTM (GE Healthcare, cat# RPN2106) and exposure by film (FUJIFILM Super RX ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired from 15 fields at 5- or 10-minute intervals using a DeltaVision Elite Deconvolution Microscope (GE Healthcare Biosciences) with a 60x oil immersion objective and a Front Illuminated sCMOS camera ...
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bioRxiv - Cancer Biology 2022Quote: ... Blood was diluted 1:2 with 1× phosphate-buffered saline (PBS) and transferred to a 50 ml falcon tube with Ficoll-Paque PLUS (GE Healthcare) at a 1:2 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Deconvolution of image stacks was performed with SoftWorxExplorer (Applied Precision, LLC). For live-cell imaging ...
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bioRxiv - Cell Biology 2022Quote: Images were acquired using an Olympus DeltaVision RT microscope (Applied Precision, LLC) equipped with a Coolsnap HQ camera and CO2 controlled chamber ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were imaged in PBS on an Applied Precision OMX Blaze V4 (GE Healthcare) microscope using a 60x 1.42 NA Olympus Plan Apo oil objective and two PCO Edge sCMOS cameras ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto PVDF membranes (Amersham Hybond 0.45 µm, GE Healthcare). For immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... and imaged with an ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The intensity of each band was quantified as a percentage of total lane signal using Image Quant TL 8.1 software (GE Healthcare Life Sciences). Oligonucleotides used are listed in Sup ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a Superdex 200 10/300 GL column using an ÄKTA chromatography system (GE Healthcare Life Sciences). 0.5 ml or 1 ml fractions were collected and analyzed by Western blotting using antibodies against HOIP and V5.
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bioRxiv - Cell Biology 2022Quote: ... The Glo3 BoCCS and GRM regions and β’-COP (residues 300-604) were ligated into BamHI/ NotI sites of pGEX-6P-1 (GE Healthcare), resulting in an N-terminal GST-tagged proteins with a 3C-protease cleavage site ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired using a DeltaVision Elite Imaging System (GE Healthcare Life Sciences, Pittsburgh, PA) equipped with a 100x objective lens ...
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bioRxiv - Cell Biology 2022Quote: GST or GST-tagged β’-COP (residues 1-604) or Glo3 constructs (GAP domain, BoCCS region, GRM region) were immobilized on glutathione sepharose resin (GE Healthcare) for one hour on ice ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were affinity purified using glutathione sepharose (GE Healthcare) or HisPur cobalt resin (Thermo Scientific ...