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Citations for GE Life Sciences :
8751 - 8800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The resulting translated proteins were resolved by a sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) gel and analyzed by phosphoimager analysis (Typhoon, Amersham, GE life sciences).
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bioRxiv - Microbiology 2022Quote: ... for anion exchange chromatography using the ÄKTA Start chromatography system (GE Healthcare) with buffer A (50 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... analytical gel filtration was performed using the Superdex™ 200 Increase 3.2/300 column (GE Healthcare). To this end ...
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bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
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bioRxiv - Immunology 2022Quote: ... The CM5 chip (GE Healthcare) was attached with our novel Sars-CoV-2 antibody to record 6000 response units ...
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bioRxiv - Immunology 2022Quote: ... Polyclonal IgG from subject 391370 was isolated from heat-inactivated plasma using Protein G Sepharose 4 Fast Flow (GE Healthcare). Purified IgG was buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were purified by Ficoll® Paque Plus (GE Healthcare, Chicago, IL) gradient centrifugation for 30 min at 2800 rpm ...
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bioRxiv - Immunology 2022Quote: ... The antibody and RBD affinity ability were estimated employing a 1:1 binding fit model in Biacore Evaluation software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Product #: 31433) (Pierce Antibodies) or donkey-anti-rabbit IgG (Product #: NA934V) (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Microbiology 2022Quote: ... the respective supernatants were applied to 1/5 ml HiTrap™ Q XL columns (GE Healthcare) for anion exchange chromatography using the ÄKTA Start chromatography system (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were pooled for buffer exchange employing a PD-10 Sephadex™ G-25 desalting column (GE Healthcare) and centrifugal filters (Amicon®Ultracel®-10 K/30 K ...
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bioRxiv - Microbiology 2022Quote: ... The protein solution was then loaded onto an equilibrated 5 mL HiTrap-Q columns (GE Healthcare) using an ÄKTA pure 25 system (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... concentrated using a 30 kDa molecular weight cut-off (MWCO) Ultra Centrifugal tube (Amicon) and loaded to a Superose 6-Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
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bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were developed using an Enhanced Chemiluminescence (ECL) reagent (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... Recombinant IgG mAbs were purified using HiTrap Protein A columns (Cytiva/GE Healthcare Life Sciences, 17040303).
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bioRxiv - Immunology 2022Quote: ... and further purified using a Superdex 200 increase 10/300 column (GE Healthcare Biosciences) in TBS buffer ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
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bioRxiv - Immunology 2022Quote: ... Images acquired by the IN Cell Analyzer 2000 were analysed using the Developer Toolbox software V1.9.2 (GE Healthcare). Data are shown as representative images or the percentage overlap of OTII T cells with DCs.
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bioRxiv - Genetics 2022Quote: ... Sequence products were purified on Sephadex G50 beads (GE Healthcare) and directly loaded onto 3130xl Genetic Analyzer capillary electrophoresis laser coupled system (ABI PRISM).
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare Biosciences AB) gradient centrifugation using SepMate-50 tubes (Stemcell Technologies Inc ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Immunology 2022Quote: ... The biotinylated proteins were further purified by gel filtration (S200, GE Life Sciences) in PBS buffer pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... 5% glucose) and the proteins purified by affinity chromatography using a HiTrap Heparin High-Performance column (GE Healthcare) and gradient elution (0-100% NaCl ...
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bioRxiv - Immunology 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin followed by gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... The sensorgrams of the three rounds were aligned from 120 to 240 s in Biacore 8K Evaluation software (GE Healthcare). The blocking efficacy was determined by a comparison of the response units with and without prior antibody injection.
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bioRxiv - Immunology 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). To determine the levels of nanobody competition with the human ACE2 and VHH72 of known epitope specificity ...
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bioRxiv - Immunology 2022Quote: ... All nanobodies were purified by gel-filtration chromatography with Superdex 200 High-Performance column (GE Healthcare). The exact concentration was determined by nanodrop 2000 Spectrophotometer (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: The binding kinetics of nanobodies to SARS-CoV-2 RBD were analyzed using SPR (Biacore 8K; GE Healthcare). The nanobodies were captured by the ProA sensor chip and serial dilutions of SARS-CoV-2 RBD were then flowed through at a rate of 30 μL/min in PBS buffer (with 0.05% Tween20 ...
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bioRxiv - Immunology 2022Quote: ... The membrane was developed with the ImmunoStar LD enhanced chemiluminescence reagents (Wako) and visualized using ImageQuant LAS 400 (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... followed by staining with the horseradish peroxidase (HRP)-conjugated anti-rabbit (1:50,000; GE healthcare) and anti-mouse (1:25,000 ...
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bioRxiv - Immunology 2022Quote: ... media was filtered through 0.2 μm PVDF filtered syringes (GE Healthcare) and centrifuged at 120,000 x g for 90 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Immunology 2022Quote: ... Membranes were revealed using the Amersham ECL prime reagent (GE healthcare) and imaged on an Amersham A600 system ...
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bioRxiv - Immunology 2022Quote: ... layered over Ficoll-Paque PLUS (GE Healthcare), and centrifuged at 2000 rpm for 20 minutes at room temperature with the brake off and the mononuclear layers were harvested.
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Cell Biology 2022Quote: ... A HiLoad® 16/60 Superdex® pg 200 (GE Healthcare) was applied for preparative runs ...
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bioRxiv - Cell Biology 2022Quote: ... Western blots were revealed with enhanced chemiluminescence (luminol and hydrogen peroxide, homemade) on an autoradiographic film (Amersham Hyperfilm™ ECL, GE Healthcare #28906837).
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bioRxiv - Cell Biology 2022Quote: ... 4A and S1A were obtained using a DeltaVision platform (Applied Precision) composed of a customized inverted microscope (IX-71 ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired using softWoRx v4.1.2 software (Applied Precision). Images were acquired every 5 minutes during 9 to 15 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoreactive bands were determined by exposing nitrocellulose blots to a chemiluminescence solution (Denville Scientific Inc., Metuchen, NJ) followed by exposure to Amersham Hyperfilm ECL (GE Healthcare Limited, Buckinghamshire, UK). To determine the relative amounts of protein densitometry Image J version 1.48v (National Institutes of Health ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed and His-ALOD4 and His-OlyA were isolated by nickel purification followed by size exclusion chromatography (HisTrap-HP Ni column, Tricorn 10/300 Superdex 200 gel filtration column; FPLC AKTA, GE Healthcare). Protein-rich fractions were pooled and concentration was measured using a NanoDrop instrument.
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bioRxiv - Immunology 2022Quote: ... The lysed cells were centrifuged and only the supernatant with the soluble proteins was then further filtered with 0.45 µm filters and loaded onto two connecting 5 mL HisTrap IMAC FF columns (GE Healthcare) which were pre-equilibrated with 50mM phosphate buffer with 500 mM NaCl and 20 mM imidazole at pH 7.4 ...
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bioRxiv - Immunology 2022Quote: Purified ORF8N78Q was loaded onto a Superose 6 (GE Healthcare) gel filtration column and eluted in 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... cleared by low-speed centrifugation and filtered through a 0.45 µm filter (GE Healthcare Whatman) to yield “lentivirus supernatant” ...
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bioRxiv - Immunology 2022Quote: ... The immune cells were obtained by density gradient centrifugation with Percoll (17-0891-09, GE Healthcare, USA) according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... anti-rabbit-HRP (GE Healthcare NA934, AB_772206), anti-goat-HRP (Sigma-Aldrich A5420 ...