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Citations for GE Life Sciences :
801 - 850 of 1376 citations for Goat Anti Rat IgG FITC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... IgG fractions were further purified by size exclusion chromatography (Superdex 200 Increase, GE Healthcare, Chicago, IL), and Fab fractions were further purified by cation exchange chromatography (MonoS ...
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bioRxiv - Microbiology 2022Quote: ... M28 IgG was then purified from the supernatant using Protein A affinity chromatography (GE Healthcare #17549851). IgG was then dialyzed overnight in TBS (150mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 3 ml of IgG Sepharose Fast Flow beads (GE Healthcare, 17096901) equilibrated with CMG buffer/300 mM KCl and incubated for 2 hours at 4°C on a rotating wheel ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was harvested and purified after 6 days with protein G sepharose beads (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Fifty microliters of a 50/50 slurry of IgG-Sepharose 6 Fast Flow beads (GE Healthcare) in tandem affinity purification buffer A was added to the supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... Dynein complexes were captured from Sf9 cell lysates using IgG Sepharose 6 FastFlow beads (GE Healthcare), labeled with SNAP-Cell-TMR-Star ...
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bioRxiv - Bioengineering 2024Quote: ... Scaffolds and RSVF trimer or IgG proteins were immobilized on a CM5 chip (GE Healthcare # 29104988) by amine coupling ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies were used as follows: Horseradish peroxidase (HRP)-conjugated mouse IgG (1:10,000; GE Healthcare) or rabbit IgG (1:3000 ...
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bioRxiv - Biochemistry 2021Quote: ... a PCR fragment containing ubiquitin gene and a PCR fragment containing rat parkin residues 77-465 were introduced into the BamHI/XhoI sites of pGEX-6P1 (GE Healthcare). The serine (S77 ...
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bioRxiv - Microbiology 2020Quote: ... AF488-labeled samples were separated by SDS-PAGE and fluorescence in gels was captured with an Amersham Imager 680 (GE Healthcare Life Sciences). To ensure that equal amounts of protein were loaded in each lane ...
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bioRxiv - Neuroscience 2022Quote: ... The probe was end labeled with 35S-dATPα using terminal deoxytransferase and extracted using Illustra Microspin G-25 columns (GE Healthcare, North America). Each slide was then hybridized with 600,000 cpms of labeled probe in 125 μl of hybridization buffer and slides incubated overnight in a humid chamber warmed to 37° C ...
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bioRxiv - Biochemistry 2024Quote: ... and the labeled enzymes were detected using Cy3 /TAMRA settings (excitation wavelength 532 nm) in a Typhoon FLA9500 phosphor imager (GE Healthcare Life Sciences). Further ...
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bioRxiv - Microbiology 2023Quote: ... probed with a 1:2,000 dilution of polyclonal α-PotA (Lin et al., 2017) followed by a 1:10000 dilution of peroxidase labeled α-mouse (GE Healthcare) and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: Both mFE- and tFE-labeled proteins were further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The dye-labeled protein was purified from unreacted free dye using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50,000 rpm for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was then subjected to first round of affinity purification with IgG-SepharoseTM (GE healthcare, Germany) for 1 hour at 4°C on the rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... while the secondary antibody (1/10,000) was a commercial Amersham ECL-HRP linked rabbit IgG (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified supernatants were then incubated with 150 mL slurry of IgG beads (GE Healthcare, 17-0969-02) for 2h at 4 °C on a turning wheel ...
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Immunology 2023Quote: ... bNAb affinity columns were made by cross-linking IgG to activated CNBr-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 25 mg of protein was incubated with 25 µl IgG-sepharose 6 FAST Flow beads (GE Healthcare) for 1 h on a rotator at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA was then detected by hybridization with α-32P-labeled probe specific for the EBV WP region and visualized with a Typhoon 9410 variable-mode imager (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Biophysics 2024Quote: ... Dyes were incubated with G-actin at a 2-fold molar excess for 2 hours at room temperature before being separated from the labeled protein by applying the labeling reaction to a spin column packed with Sephadex G-50 Fine DNA Grade (GE Healthcare GE17-0573-01) hydrated with A buffer (5 mM Tris-HCL (pH 8) ...
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bioRxiv - Biophysics 2024Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM BME) and eluted with 10-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was clarified by centrifugation (65,000 rpm for 45 min) and incubated with IgG Sepharose (GE Healthcare) for 2 h at 12 °C ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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A tyrosine kinase protein interaction map reveals targetable EGFR network oncogenesis in lung cancerbioRxiv - Cancer Biology 2020Quote: ... The supernatant was incubated with 60 μl preclearing beads (mouse IgG agarose, SIGMA or Sepharose 4FF, GE Healthcare) for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Immunology 2022Quote: IgG was purified by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences, 17040203) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Biochemistry 2019Quote: ... mTLR9-ECD was purified by protein A-affinity chromatography with IgG Sepharose 6 Fast Flow resin (GE Healthcare), washed with PBS and eluted with 0.1 M glycine-HCl pH 3.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared supernatant was incubated with 250 μL of pre-equilibrated IgG beads (GE Healthcare, Little Chalfonte, UK) at 4°C for 1.5 h with gentle rotation ...
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bioRxiv - Immunology 2020Quote: ... Fab fragments were purified by removing IgG and Fc contaminants using a HiTrap MabSelectSure (GE Healthcare Life Sciences) column according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Complexes were incubated with 10–15 μg of SARS-CoV-2 spike and ∼1 mg of purified polyclonal Fab ...
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bioRxiv - Immunology 2020Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Final Fab yields were ~0.75-1.5 mg ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931 ...
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Clear lysate was incubated with 0.5 ml packed beads IgG Sepharose 6 Fast-Flow (GE Healthcare, Chicago, IL) for 2-3 hours at 4°C on a tube roller ...
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bioRxiv - Cell Biology 2024Quote: ... which was probed with a horseradish peroxidase-coupled secondary antibody (donkey-α-rabbit IgG, GE HealthCare, 1:400). Subsequent staining was performed using 3-amino-9-ethylcarbazol ...
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bioRxiv - Immunology 2023Quote: ... The bNAb affinity columns were made by cross-linking IgG to activated CNBr-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s instructions and as previously described (83) ...
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bioRxiv - Molecular Biology 2024Quote: Clarified lysate was added to 2.3 mL of a 50% IgG Sepharose 6 Fast Flow resin (GE Healthcare) slurry and incubated on a roller for 1 hour ...