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Citations for GE Life Sciences :
601 - 650 of 2886 citations for 8 HYDROXY 2 METHYL 4 1H PYRAZOL 1 YL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... This was then coupled onto NHS-ester Sepharose 4 Fast Flow resin (GE Healthcare, 17-0906-01) for 4 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... samples were incubated with anti-LptD antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) at 4°C over-night with slow rotation ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was nutated (2h, 4°C) with 10ml glutathione-Sepharose 4B resin (GE Healthcare, Pittsburg, PA) in resuspension buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were separated by 4-20% SDS-PAGE and transferred to PVDF membranes (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% BSA in 1x TBS with 0.1% Tween 20 for 1 hour at room temperature with shaking ...
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bioRxiv - Biochemistry 2021Quote: Aβ42 monomers were immobilized by amine coupling onto flowcell 4 on a CM5 sensor chip (GE Healthcare) using a BIAcore 3000 instrument (BIAcore AB) ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were re-adsorbed onto a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) and the flow-through was purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2021Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Plant Biology 2022Quote: ... crude extracts (4 ml) were passed through 0.5 ml DEAE-Sephadex A-25 columns (GE Healthcare, USA). The bound GSL was digested on the column for 18 h at room temperature with ∼0.8U myrosinase/thioglucosidase ...
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bioRxiv - Molecular Biology 2024Quote: ... Femurs were placed in a 4-limb positioning jig and scanned in eXplore Locus scanner (GE Healthcare). The femur containing jig was immersed in water and specimens were scanned at an 18 µm voxel size using the following settings ...
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bioRxiv - Microbiology 2023Quote: ... of which 450 µl were subjected to isoelectric focusing on pH 4-7 IPG strips (GE Healthcare), followed by SDS-PAGE with 12 % acrylamide gels ...
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bioRxiv - Immunology 2023Quote: ... The secreted antibodies in the supernatant were purified by protein G Sepharose 4 Fast Flow (GE Healthcare). Purified antibodies were dialyzed in PBS and sterile filtered (0.22 μm) ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Neuroscience 2023Quote: The binding of FUS to (G4C2)4 RNA was analyzed using a Biacore T200 instrument (GE Healthcare). (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K ...
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bioRxiv - Cell Biology 2024Quote: ... coli BL21 Star (DE3) and affinity-purified on a Glutathione Sepharose 4 Fast Flow column (GE Healthcare), followed by ion-exchange chromatography on a POROS GoPure HQ column (Applied Biosystems ...
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bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl of a Protein A sepharose (nProtein A Sepharose 4 fast flow, GE Healthcare, 17-5280-01) suspension was added and incubated for 1 hour at 4°C on a rotator ...
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bioRxiv - Cell Biology 2020Quote: Sheared chromatin was pre-cleared with 50 µl protein A Sepharose 4 Fast Flow beads (GE Healthcare, Germany) per 500 µg chromatin for 2h ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: Recombinant ATG8 proteins were then coupled to cyanogen bromide-activated sepharose 4 Fast Flow (CNBr-sepharose, GE Healthcare) as described (Kavran & Leahy ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C) the sample was applied onto a HiLoad Superdex 200 (16/60) column (GE Healthcare, now Cytiva). Separation was performed at a flow rate of 0.8 mL/min in SEC buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Images (4 fields/well at 20x magnification) were captured through an INCell Analyzer 6000 HCA system (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Biochemistry 2019Quote: Removal of the His6-tag (Rab8a) was performed by overnight incubation at 4 °C with thrombin (GE Healthcare), followed by a second Ni2+-agarose column ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied and circulated on a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) for 16 h ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... followed by size-exclusion chromatography at 4 °C on a HiLoad Superdex 200 pg (GE Healthcare Life Sciences) column pre-equilibrated in the same buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... incubated overnight at 4°C and subjected to size exclusion chromatography using Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing target protein were pooled and incubated over night at 4°C with PreScission protease (GE Healthcare) at a ratio of 1:100 (w/w ...
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bioRxiv - Bioengineering 2024Quote: The RSVF-multi-4 immunogen was purified by size exclusion chromatography using a Superdex200 26 600 (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 and 150 mM NaCl and concentrated to ∼20 mg/ml (Amicon Ultra-15 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 mM NaCl and 2 mM DTT on a Superdex 200 column (GE Healthcare). In case of individual purification of PHF14 and HMG20A ...
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bioRxiv - Plant Biology 2019Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Microbiology 2019Quote: ... tuberculosis CFP was purified and concentrated using 2-D Clean-Up Kit (GE Healthcare) and resuspended in 125 µl of rehydration solution (urea 7M ...