Labshake search
Citations for Quantifoil Micro Tools :
1 - 50 of 482 citations for Nnn Unlabeled 2 Mg Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... 0.5 μL L-PEG-L (8 mg/ml) and 0.5 μL Pd(NO3)2 (1 mg/ml) was applied to holey carbon grid (Quantifoil 200 mesh Cu R2/2) which was glow discharged 45 s at 15 mA ...
-
bioRxiv - Biophysics 2021Quote: 4 µL of purified HsPepT1 at 2 mg/mL was applied to a glow discharged gold holey carbon 2/1 300-mesh grid (Quantifoil). The grid was blotted for 4 s at 0 force before being plunge vitrified in liquid propane using a Mark IV Vitrobot (ThermoFisher) ...
-
bioRxiv - Biochemistry 2024Quote: ... of the freshly eluted complex peak solution (4.0 mg/mL) was loaded onto glow-discharged holey carbon grids (Quantifoil 2/2). The procedures for blotting and plunge freezing the grids were the same as above ...
-
bioRxiv - Biophysics 2019Quote: 3.5 μl of sample of fragmented doublets (~4 mg/ml) or the Sarkosyl A-tubule (~500 μg/ml) was applied to a glow-discharged holey carbon grid (Quantifoil R2/2), blotted and plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... 3 μl of the sample at 2 mg/ml were applied to 300 mesh UltrAuFoil Holey Gold Films R1.2/1.3 (Quantifoil) that had been previously plasma cleaned in a 75% argon / 25% oxygen atmosphere at 15 Watts for 6 seconds using a Solarus plasma cleaner (Gatan ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 μl of KMN complex at 0.3 mg/ml supplemented with 0.003% Triton were applied to glow-discharged UltrAuFoil R2/2 grids (Quantifoil). Excess liquid was removed by blotting (3.5 s at blot force −3) ...
-
bioRxiv - Biophysics 2020Quote: Three microliters of purified HCoV-NL63 virions (4.5 mg/mL) were first applied to 300-mesh R2/1 + 2 nm C-film grids (Quantifoil) that had been glow-discharged for 15s PELCO easiGlow (TED PELLA ...
-
bioRxiv - Biophysics 2023Quote: ... 4 μl of 4.3 mg/ml Slo1-γ1 complex were applied to glow-discharged UltrAuFoil R2/2 200 grid (Quantifoil). Any excess liquid was removed by blotting for 3.5 s at a blot force of −3 ...
-
bioRxiv - Biophysics 2021Quote: ... of purified CtenDNAV-II virons (10 mg ml-1) was deposited onto freshly glow-discharged holey carbon-coated copper grids (Quantifoil R 2/2, 300 mesh, copper) followed by 2 s of blotting in 100% relative humidity for plunge-freezing (Vitrobot Mark IV ...
-
bioRxiv - Biophysics 2020Quote: ... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
-
bioRxiv - Biophysics 2019Quote: 3 µL of purified aldolase (1.6 mg/mL) or apoferritin (5 mg/mL) was dispensed on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for six seconds at 15Watts (75% nitrogen/25% oxygen atmosphere ...
-
bioRxiv - Molecular Biology 2023Quote: ... Aliquots of 3 μL of samples at 0.75 mg/ml or 1.5 mg/ml protein concentration were placed on 300-mesh Cu holey carbon grids (Quantifoil, R1.2/1.3) after glow-discharge (PELCO easiGlow™ ...
-
bioRxiv - Biophysics 2019Quote: 3.5 ul of sample of doublets (∼4 mg/ml) was applied to a glow-discharged holey carbon grid (Quantifoil R2/2), blotted and plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 25 mM Tris at pH 8.0 to a concentration of 2 mg/mL to a glow-discharged (1 min at 5 mA) holey carbon grid (Quantifoil Q225-CR1.3). This was followed by plunge-freezing in liquid ethane using an FEI Vitribot Mark IV (100% humidity ...
-
bioRxiv - Biophysics 2019Quote: 3.5 μl of microtubule fraction sample (∼4 mg/ml) purified from WT Chlamydomonas was applied to glow-discharged holey carbon grids (Quantifoil R2/2), blotted and vitrified in liquid ethane using the Vitrobot Mark IV (FEI Company) ...
-
bioRxiv - Cell Biology 2020Quote: ... 3.5 µL of gel filtration column eluate (∼0.5 mg/ml) was applied to a holey carbon support (Quantifoil 2/1-4Cu-50) at ∼90% relative humidity ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 µl of MHAS2168-Mb_H2 complex (9.4 mg/ml and 6 mg/ml) were applied to glow-discharged (45 s) holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh) and a Grid Plunger GP2 (Leica ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 0.73 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R2/2, 300 mesh). After blotting for 3 s ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2.4 μl of 0.12 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2.8 µl of 0.10 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
-
bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Biochemistry 2021Quote: ... or 0.3 mg/ml for Au-foil grids (Quantifoil, Jena, DE). 4ul of sample was applied to glow-discharged grids ...
-
bioRxiv - Biochemistry 2021Quote: ... 2.5-μL aliquots of purified Neo1 at a concentration of about 1 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
-
bioRxiv - Biochemistry 2019Quote: 2.5 µl of freshly purified protein at a concentration of 3.3 mg/ml when solubilized in DDMand at about 2 mg/ml when reconstituted in nanodiscs were applied on holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200, 300 and 400 mesh), which were prior glow-discharged at 5 mA for 30 s ...
-
bioRxiv - Immunology 2020Quote: ... four-microliter aliquots of Fcα-J-SC (0.3 mg/ml) or Fcα-J-SC-SpsANTD (0.2 mg/ml) were applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3), blotted with filter paper at 4 °C and 100% humidity ...
-
bioRxiv - Biochemistry 2022Quote: ... 2.5 μL of the purified PTH-PTH1R-Gs and PTHrP-PTH1R-Gs complexes at a concentration of ∼8.4 mg/mL and ∼5.1 mg/mL were respectively applied to the glow-discharged Au 300 mesh holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted and then plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biophysics 2023Quote: 2.5μL of ∼1.2 mg/ml sample were applied to UltraAuFoil 1.2/1.3 300 mesh (Quantifoil) or 1.2/1.3 Cu 300-mesh grids (Quantifoil ...
-
bioRxiv - Cell Biology 2020Quote: ... 3.5 μL of the peak fraction from a BBSome purification (0.4 - 0.6 mg/mL) was applied to glow-discharged R1.2/1.3 holey carbon copper grids (Quantifoil) covered with a thin homemade carbon film ...
-
bioRxiv - Biochemistry 2021Quote: 2.5 μl of ACE2-RBD-62 complex at 3.5 mg/ml concentration was transferred to glow discharged UltrAuFoil R 1.2/1.3 300 mesh grids (Quantifoil), blotted for 2.5 seconds at 4°C ...
-
bioRxiv - Biophysics 2020Quote: 3 ml of apoferritin at 3.8 mg/ml were applied to freshly glow discharged R 1.2/1.3 holey carbon grids (Quantifoil) at 4°C and 100% relative humidity followed by plunge-freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... 3.5 µL of purified FERRY complex at a concentration of 0.7 mg/mL was applied to freshly glow-discharged UltrAuFoil 1.2/1.3 grids (Quantifoil), automatically blotted for 3 s and plunged into liquid ethane using the a Vitrobot (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2023Quote: 3 μl purified MRS2 at approximately 0.5 mg/ml was applied to a glow-discharged 400-mesh R 1.2/1.3 Cu grid (Quantifoil). The cryo grids were blotted for 6 s at 4°C and 95% ...
-
bioRxiv - Biophysics 2023Quote: We applied 3 μl of purified MeFDH1 (0.5 mg/ml) to a 1.2/1.3 holey carbon 200 mesh grid (Quantifoil), blotted for 4 s in a 100% humidity environment at 12 °C and then froze the specimens by plunging them into liquid ethane using a Vitrobot Mark IV (FEI).
-
bioRxiv - Biochemistry 2024Quote: A volume of 3 µl purified protein complex at a concentration of 1mg/ml (JSR1-hGi) or 8 mg/ml (JSR1-jsGiq) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Cu 200 mesh). The grids were blotted with a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: A droplet of 3 μl of purified GluD2 Δ870 at a concentration of ~0.8 mg/ml was applied onto a glow discharged 1.2/1.3 300 mesh ultra foil gold grid (Quantifoil). It was blotted for 3 seconds at a blot force of 0 using the FEI Vitrobot Mark IV at 14°C and 95 % humidity ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3.5 μL of purified protein at a concentration of 0.22 mg/mL was applied to Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil), and then plunged into liquid nitrogen-cooled liquid ethane with a FEI Vitrobot Mark IV (FEI Thermo Fisher) ...
-
bioRxiv - Biochemistry 2022Quote: ... concentrated to ~3.5 mg/ml was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which were glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Biochemistry 2021Quote: ... 4 μL sample (0.08-0.15 mg/ml) was then spotted on glow discharged Quantifoil holey grids (R2.2, Cu 300 mesh, Quantifoil Micro Tools GmbH ...
-
bioRxiv - Biophysics 2020Quote: 0.8 mg/mL aldolase solution was applied to glow-discharged gold holey carbon 1.2/1.3 200 mesh grids (Quantifoil). Grids were blotted for 3 seconds at 0 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher) ...
-
bioRxiv - Biophysics 2021Quote: ... 3.5 μl of purified protein at a concentration of 1.0 mg/ml was applied to Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil), and then plunged into liquid nitrogen-cooled liquid ethane with a FEI Vitrobot Mark IV (FEI Thermo Fisher) ...
-
bioRxiv - Immunology 2022Quote: ... 2.2 µl of the purified mixture at concentration of 0.2 mg/mL was applied to glow discharged (40 s at 25mA) UltraAu grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 µl of DELE WT CTD sample at 3 mg/ml was applied onto 300 mesh R1.2/1.3 UltraAuFoil holey gold grids (Quantifoil) that were plasma cleaned for 15 s using Pelco glow discharge cleaning system (Ted Pella ...
-
bioRxiv - Biophysics 2023Quote: ... Aliquots of 4 μl of protein samples (0.3 mg ml-1 or ∼6 μM) were applied to glow-discharged R1.2/1.3 200 Cu mesh grids (Quantifoil). After 30 s ...
-
bioRxiv - Microbiology 2023Quote: ... 2.2µl of the purified mixture at a concentration of 0.5 mg/mL was applied to glow discharged (30 s at 25mA) grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3.5 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
-
bioRxiv - Neuroscience 2023Quote: ... Double application of 2 µL protein (∼0.9 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (1.2/1.3, 200 mesh, Quantifoil). Grids were blotted for 3 s and 10 s ...
-
bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex (8 mg/ml) was deposited onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3 ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein was diluted from 0.1 mg/ml to 0.05 mg/ml with deionized water and loaded onto a freshly glow-discharged Quantifoil R1.2/1.3 Cu grid (Quantifoil Micro Tools GmbH ...