Labshake search
Citations for Quantifoil Micro Tools :
1 - 50 of 411 citations for HLA A*0201 NY ESO 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... 3.5 μl aliquots of the recombinant human TIM22 complex (7 mg ml-1) were dropped onto glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the protein complex was applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey gold support films (Quantifoil). For cryo-EM grids preparation of DHA-FFA4-miniGq complex ...
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bioRxiv - Biochemistry 2023Quote: Purified ternary complex was vitrified on holey-carbon grids (Quantifoil R 2/1) using FEI Vitrobot Mark IV ...
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bioRxiv - Bioengineering 2020Quote: ... Aliquots (3.3 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). The grids were blotted for 2.5 s or 3.0 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Biochemistry 2023Quote: ... An aliquot of 4 μL protein sample of Polθ-HLD-NVB complex at a protein concentration of 0.92 mg/mL was loaded onto a glow-discharged 400 mesh grid (Quantifoil Au R1.2/1.3), blotted with filter paper for 3.0 s and 3 blot force ...
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bioRxiv - Biophysics 2021Quote: ... 2.5 μL of each purified CCKAR–G protein complex was applied individually onto the glow-discharged holey carbon grids (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of purified PP2A-PME-1 complex was applied onto a glow-discharged holey carbon grid (Quantifoil 300 mesh R 1.2/1/3 with ultrathin carbon ...
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bioRxiv - Biophysics 2021Quote: A 3.5 µL droplet of purified TF55β complex at either 20.0 mg/mL (ATP-complex) or 12.0 mg/mL (ADP-complex) was applied to a copper grid with holey carbon film (Quantifoil Copper R1.2/1.3, 200 mesh) that had been glow discharged for 60 s ...
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bioRxiv - Biochemistry 2023Quote: 3.5 µl purified TOL-1–LAT-1 full-ectodomain complex (0.28 mg/ml) was applied on glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 200 mesh), and vitrified using a Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Neuroscience 2023Quote: ... Double application of 3 µL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Immunology 2020Quote: An aliquot of 3 μl purified protein of rNLRP1-rDPP9 or rNLRP1FIIND S969A-rDPP9 complex was applied to holey carbon grids (Quantifoil Au 1.2/1.3, 300 mesh), which were glow-discharged for 30 s at middle level in Harrick Plasma after 2 min evacuation ...
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bioRxiv - Molecular Biology 2021Quote: ... DRD1-SKF81297-LY3154207-miniGs-Nb35 complex at the concentration of 13 mg mL−1 were applied individually onto glow-discharged holey carbon grids (Quantifoil, Au200 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Molecular Biology 2023Quote: ... a 4 µl sample of purified complex was applied to glow-discharged R2/1 300 mesh holey carbon copper grids (Quantifoil Micro Tools GmbH), using a conc ...
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bioRxiv - Microbiology 2019Quote: 3 μl of purified complex at 1 to 3.6 mg/ml were applied to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3). Grids were blotted for 3 s at 100% humidity at 22 °C and frozen in liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2020Quote: Samples were prepared for CryoEM by applying 5 μl freshly purified Cx46/50-lipid nanodisc complex (∼2.5 mg mL-1) to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh) for 10 seconds ...
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bioRxiv - Molecular Biology 2019Quote: ... D2-I or D2 complex were blotted on plasma cleaned UltraAufoil R1.2/1.3 grids31 (Quantifoil) for 3–4.5 s and plunged into liquid ethane using a Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2021Quote: ... In each case 3μl of the complex was applied to grids (Quantifoil R1.2/1.3 300 mesh Au) and plunge frozen using a Thermo Fisher Scientific Vitrobot MKIV ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μL of freshly purified CasΦ3 R-loop complex (Absorbance260 nm of ~1.6) was applied to UltrAuFoil 300 mesh R0.6/1.0 holey grids (Quantifoil), glow-discharged for 60 s at 10 mA (Leica EM ACE200) ...
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bioRxiv - Biochemistry 2023Quote: 3.5 μL of LRRK2RCKW:E11 complex was applied to glow-discharged UltraFoil 300 mesh R1.2/1.3 grids (Quantifoil) and incubated in a FEI Vitrobot IV at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.5 μL aliquot of the dialyzed complex was deposited onto gold UltrAuFoil R1.2/1.3 Au 300 mesh cryo-electron microscopy grids (Quantifoil) and plunge frozen into liquid-nitrogen cooled liquid ethane using a Mark IV Vitrobot (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: 3.5 microliters of Grafix purified complex with ARE35 DNA (Fraction 11) was applied to glow discharged R1.2/1.3 holey carbon grids (Quantifoil) at 4ºC and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermofisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... 3 µl of TIR-SAVED complex was applied to Holey Carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh), glow discharged for 45 s at current of 45 mA in a EMITECH K100X glow discharger ...
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bioRxiv - Immunology 2022Quote: ... 4.0 µl of the complex was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlowTM Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the purified complex was applied to glow-discharged holey carbon grids (Quantifoil, Au300 R1.2/1.3). Grids were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fischer Scientific).
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bioRxiv - Biophysics 2023Quote: The freshly purified CD69-S1PR1-Gi-scFv16 complex was added to Quantifoil R1.2/1.3 400-mesh Au holey carbon grids (Quantifoil), blotted using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biochemistry 2022Quote: 2.75 μL of purified OR51E2-Gs complex was applied to glow discharged 300 mesh R1.2/1.3 UltrAuFoil Holey gold support films (Quantifoil). Support films were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: The purified OR–Gs complex was applied to glow-discharged 300 mesh R1.2/1.3 UltrAuFoil Holey gold support films (Quantifoil). Support films were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Double application of 2 µL protein (∼0.9 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (1.2/1.3, 200 mesh, Quantifoil). Grids were blotted for 3 s and 10 s ...
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bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex (8 mg/ml) was deposited onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3 ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µL of the resuspended solution containing the MagIC-cryo-EM beads isolated interphase-specific H1.8-GFP containing complex were applied to plasma-cleaned in-house graphene attached Quantifoil gold R1.2/1.3 300-mesh grids (Quantifoil). The grid was then incubated on the 40 x 20 mm N52 neodymium disc magnets for 5 min and vitrified using the Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2024Quote: The purified complex was applied onto freshly glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3), which were then immediately plunge-frozen in liquid ethane ...
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bioRxiv - Biophysics 2021Quote: ... 2.5 μl of the complex were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). Samples were blotted for 2-4 s at 4 °C and 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: 3.0 μl of purified complex was applied to glow-charged 300 mesh holey carbon grid (Quantifoil Au R1.2/1.3). Grids were blotted for 3.0-4.0 s at a blotting force of 4 and vitrified using a Vitrobot MarkIV (Thermo fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μl of complex mixture was applied on glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 200 mesh) and excess of liquid was removed by blotting for 2-4 s in controlled environment (4°C ...
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bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
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bioRxiv - Biophysics 2022Quote: ... 2.5 μl of the complex were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). Samples were blotted for 2-4 s at 4 °C and 100% humidity ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 4.3 mg/ml Slo1-γ1 complex were applied to glow-discharged UltrAuFoil R2/2 200 grid (Quantifoil). Any excess liquid was removed by blotting for 3.5 s at a blot force of −3 ...
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bioRxiv - Cell Biology 2023Quote: ... Two μl of the diluted complex were applied to freshly glow-discharged R 2/2 holey carbon grids (Quantifoil), which were blotted with a force of 6 for 5 seconds and immediately vitrified by plunge-freezing in liquid ethane using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Biophysics 2019Quote: An aliquot of 3.5 μL GCGR-Gs-Nb35 complex was applied to glow-discharged 200 mesh grids (Quantifoil R1.2/1.3), at a concentration of 17 mg/ml and subsequently vitrified using a Vitrobot Mark IV (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: The 2.5 µl sample of purified NP-RNA complex solution was applied onto glow-discharged 200-mesh R1.2/1.3 Cu grids (Quantifoil, Jena, Germany). The grids were blotted and rapidly frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Samples were diluted to a concentration of 60-65 nM for the Pol II-U1 snRNP complex and 2 μl was applied to each side of the R2/2 UltrAuFoil grids (Quantifoil) that had been glow-discharged for 100 s ...
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bioRxiv - Biochemistry 2020Quote: ... at 4.0 mg/ml and INT-777–GPBAR–Gs complex (3.0 μL) at 4.5 mg/ml were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), and subsequently vitrified using a Vitrobot Mark IV (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots (3.3 μL) of the S/2G1 complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). The grids were blotted for 2.5 s or 3.0 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV ...
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bioRxiv - Molecular Biology 2023Quote: Cryo-EM grids preparation for the isolated BRCA1ΔExon11:FL BARD1 complex: UltrAuFoil R1.2/1.3 300-mesh gold grids (Quantifoil Micro Tools GmbH) were cleaned via indirect plasma using a Tergeo EM plasma cleaner (Pie Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 3 or 3.5 μl of LRRK2RCKW:inhibitor:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex were loaded onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3), and subsequently were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: The purified PACAP27–VIP2R–Gs complex (3 μL at 3.7 mg per mL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 3 μl of spike-Fab complex was applied to glow-discharged (20 mAmp, 30 sec, Quorum GloQube) Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH), blotted for 5 s using blot force 2 and plunge frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...