Labshake search
Citations for Quantifoil Micro Tools :
701 - 750 of 751 citations for 6 Chloro 2 4 chlorophenyl N N dimethylimidazo 1 2 α pyridine 3 methanamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... The complex (1 mg/mL) was then applied onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3) using an FEI Vitrobot IV ...
-
bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
-
bioRxiv - Cell Biology 2021Quote: MEFs expressing emerald-Vimentin are cultured on glow-discharged holey carbon coated EM grids (Au R2/1, 200 mesh, Quantifoil) for 16 h at 37°C in a humidified CO2 incubator ...
-
bioRxiv - Biochemistry 2022Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/ml was applied to a glow-discharged Quantifoil 300 mesh copper R1.2/1.3 grids (Quantifoil Micro Tools). The grids were then flash frozen in liquid ethane using a Thermo Fisher Scientific Vitrobot IV (0-0.5 s waiting time ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples of ΦKZ nvRNAP were adsorbed to a thin film of graphene oxide deposited upon the surface of holey carbon copper grids (R2/1, 300 mesh, Quantifoil). Grids were blotted for 1-2 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: An aliquot of 2.5 microliters of purified drCALHM1 sample was applied onto a glow discharged holey carbon film grid (200 mesh, R2/1, Quantifoil). The grid was blotted and flash-frozen in liquid ethane with FEI Vitrobot Mark IV ...
-
bioRxiv - Cell Biology 2020Quote: ... Isolated centrioles were then deposited onto 200-mesh copper EM grids coated with holey carbon (R3.5/1, Quantifoil Micro Tools) and plunge-frozen in a liquid ethane/propane mixture ...
-
bioRxiv - Cell Biology 2021Quote: K5/K14_1 cells were seeded on glow-discharged Collagen I coated holey carbon gold EM grids (Au R2/1, 200 mesh, Quantifoil) and incubated overnight at 32 °C and in 5% CO2 ...
-
bioRxiv - Cell Biology 2020Quote: Samples of concentrated TSCCFL were adsorbed to a thin film of graphene oxide deposited upon the surface of holey carbon copper grids (R2/1, 300 mesh, Quantifoil). Grids were blotted for 2-3 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... Three μL of sample at 5 μM was adsorbed onto glow-discharged copper Quantifoil R2/1 holey carbon grids (Quantifoil). Grids were glow discharged 45 seconds at 25 mA using an EMITECH K950 apparatus ...
-
bioRxiv - Cell Biology 2023Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/mL was applied to UltrAuFoil 300 mesh gold R1.2/1.3 grids (Quantifoil Micro Tools), freshly glow discharged with an Edwards S150B glow discharger for 1 min 15 sec ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3) ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 µl of the VIF-ΔT solution was applied onto glow-discharged holey carbon EM grids (Cu R2/1, 200 mesh, Quantifoil), which were subsequently blotted manually and plunge frozen in liquid ethane.
-
bioRxiv - Biochemistry 2023Quote: ... 2.8 µl of F-actin sample was applied to a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). Excess solution was blotted away and the grids were plunge frozen in liquid ethane or a liquid ethane/propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2022Quote: ... A total of 3.5 μL of TP–AG treated MT samples was applied to a glow discharged holey carbon grid (Quantifoil R2/1). The grids were blotted ...
-
bioRxiv - Cell Biology 2021Quote: ... a thin layer of homemade continuous carbon was floated on top of individual Quantifoil R3.5/1 200 mesh gold grids (Quantifoil Micro Tools). After drying ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 μl of samples were applied to glow-discharged Quantifoil R 0.6/1 and R 1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany), blotted for 3 s and then flash frozen in liquid ethane using the automatic plunge freezer EM GP2 at 100% relative humidity ...
-
bioRxiv - Cell Biology 2021Quote: MEFs were grown to ~80% confluency on glow-discharged holey carbon EM grids (R2/1, Au 200 mesh; Quantifoil, Jena, Germany) prior to preparation for cryo-ET analysis ...
-
bioRxiv - Immunology 2022Quote: A 3.5 μL sample of concentrated pNO-decorated CLPs was applied to negatively glow discharged (25 mA, 40 s) R3.5/1 quantifoil copper grids (Quantifoil Micro Tools). The excess of solution was blotted using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Biochemistry 2023Quote: ... at a concentration of around 2.5 mg ml−1 were applied to glow-discharged 300-mesh Au grids (Quantifoil, R1.2/1.3). Excessive sample was removed by blotting with filter paper for 3.5 s before plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 8 °C.
-
bioRxiv - Biochemistry 2023Quote: MEFs were grown to ∼80% confluency on glow-discharged holey carbon EM grids (R2/1, Au 200 mesh; Quantifoil, Jena, Germany) prior to preparation for cryo-ET analysis ...
-
bioRxiv - Microbiology 2020Quote: ... of purified proteins of SARS-CoV2-ST or SARS-CoV2-STN were loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R2/1, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: Cells of strain Pla133T were mixed with the same volume of BSA-stabilized 15 nm colloidal gold solution (Aurion) and placed on holey carbon-coated 200 mesh copper grids (R2/1, Quantifoil, Jena, Germany) immediately before thin-film vitrification by plunge-freezing in liquid propane (63%)/ethane (37% ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... A 3.5 µL virus sample solution was applied onto a glow discharged copper grid coated with holey carbon (R2/1; Quantifoil, Jena, Germany) prior to plunge-freezing ...
-
bioRxiv - Biochemistry 2024Quote: ... Peak fractions were concentrated to ∼3.2 mg/mL and 3.5 μL of concentrated sample immediately applied to a Quantifoil R2/1 300 copper mesh grid (Quantifoil Micro Tools GmbH), which had been glow discharged in a GloQube Plus glow discharger for 45 seconds at 25 mA and coated with graphene oxide (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein-DNA complexes were applied to ultra-gold foil grids with holey gold support and 300 mesh size (R 0.6/1,Quantifoil Micro Tools GmbH); grids were flash-frozen as described [21] and were stored in liquid nitrogen until use.
-
bioRxiv - Cell Biology 2023Quote: ... 4 µL of cell suspension was applied on 200-mesh R ¼ SiO2-film covered gold grids or 200-mesh R2/1 carbon-film covered copper grids (Quantifoil Micro Tools) (for P ...
-
bioRxiv - Microbiology 2023Quote: ... 3µL of each sample was was pipetted onto a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany), then blotted to remove excess sample and plunge-frozen into a mixture of liquid ethane and propane cooled in a bath of liquid nitrogen using a Vitrobot Mk 4 cryo-plunger (41) ...
-
bioRxiv - Biochemistry 2024Quote: Samples were prepared by applying the polymerized R-β-Actin actin to freshly glow-discharged Quantifoil 3.5/1 on 200 mesh copper grids (Quantifoil GmbH, Germany), using a Leica GP2 (Leica Microsystems GmbH ...
-
bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
-
bioRxiv - Molecular Biology 2021Quote: ... DRD1-SKF81297-LY3154207-miniGs-Nb35 complex at the concentration of 13 mg mL−1 were applied individually onto glow-discharged holey carbon grids (Quantifoil, Au200 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
-
bioRxiv - Biochemistry 2022Quote: Three microliters of Ct80S-RAC pull-out sample at 200 nM concentration was applied on holey carbon grids (Quantifoil R2/1 grid, Quantifoil Micro Tools, GmbH) and plunged-frozen into liquid ethane using a Vitrobot (FEI) ...
-
bioRxiv - Microbiology 2020Quote: Usually 5 μL of liquid culture (OD680 ~ 0.3) was pipetted onto holey carbon-coated copper grids (R2/1, 200 mesh, Quantifoil Micro Tools, Jena, Germany). The grids were blotted from the reverse side and immediately plunged into the liquid ethane at liquid nitrogen temperature atmosphere using a Vitrobot ...
-
bioRxiv - Microbiology 2020Quote: ... 3.5 microliters of the purified virus sample was pipetted onto a glow-discharged R2/1 Quantifoil grid (Quantifoil Micro Tools GmbH, Jena, Germany), blotted for 2.5 seconds ...
-
bioRxiv - Microbiology 2022Quote: ... 5 µl of bacteria diluted to OD = 0.15 in PBS were deposited on a glow-discharged Quantifoil R2/1 holey carbon grids (Quantifoil Micro Tools GmbH, Germany) and 3 µl gold particles (10 nm BSA tracer ...
-
bioRxiv - Biochemistry 2022Quote: ... three microliters of concentrated phage particles were added to a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany) and plunge-frozen with a Vitrobot Mk IV into a 50:50 mixture of liquid ethane:propane52
-
bioRxiv - Microbiology 2022Quote: Three-microliter aliquots of all AdSDV capsid populations (~1 mg/mL) were applied to glow-discharged Quantifoil holey carbon grids with a thin layer of carbon (Quantifoil Micro Tools GmbH) and vitrified using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Biochemistry 2024Quote: ... 3.5 μL of this protein-DNA mix were applied to 300-mesh copper R2/1 Quantifoil Holey Carbon Grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 25 mA in a GloQube Glow Discharge System (Quorum Technologies ...
-
bioRxiv - Microbiology 2023Quote: 3 µL of the purified FL or truncated RSV NC sample were applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Microbiology 2019Quote: 3 μl of purified complex at 1 to 3.6 mg/ml were applied to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3). Grids were blotted for 3 s at 100% humidity at 22 °C and frozen in liquid ethane using a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Microbiology 2021Quote: ... 5 µl of the cell suspension was placed on freshly glow-discharged (for ∼25 s) holey carbon grids (Quantifoil Cu R2/1, 200 mesh). The grids were front blotted with Whatman filter paper and rapidly frozen in liquid ethane ...
-
bioRxiv - Biophysics 2020Quote: Samples were prepared for CryoEM by applying 5 μl freshly purified Cx46/50-lipid nanodisc complex (∼2.5 mg mL-1) to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh) for 10 seconds ...
-
Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: ... 3.0 µl aliquots at a concentration of 0.87 or 1.0 mg/ml of the spikeD614G-binder sample or the spikeOmicron-binder sample were applied onto glow-discharged carbon-coated copper grids (Quantifoil R2/1, 400 mesh), blotted for 4.0-8.0 s ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3), after which ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 μL of ∼100 nM (A260 4.0 or 6.0) mitoribosome sample was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/1 copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
-
bioRxiv - Biochemistry 2024Quote: ... Approximately 3.5 μl of the solution was applied onto freshly glow-discharged and graphene oxide-coated TEM grids (Quantifoil R2/1, Cu 200 mesh) and plunge-frozen into liquid ethane by a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... LCNP or EV suspensions in PBS (∼1 × 1012 particles/mL) were deposited on 300-mesh lacey carbon-coated copper grids (Quantifoil Micro Tools GmbH, Germany). The grids were previously glow-discharged for 45 s using an Emitech K100X glow discharge system (Quorum Technologies Ltd. ...
-
bioRxiv - Biophysics 2020Quote: Three microliters of the apoferritin samples with 1.5 mg/ml or 0.2 mg/ml were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (used for the K3 dataset) or 200-mesh R2/1 Quantifoil grids coated with continuous carbon film (Quantifoil, used for the Falcon4 dataset), respectively ...
-
bioRxiv - Biophysics 2019Quote: ... 3.5 μl aliquots of the recombinant human TIM22 complex (7 mg ml-1) were dropped onto glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...