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Citations for G-Biosciences :
1 - 50 of 63 citations for St Louis Encephalitis virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate was purified over a Ni-NTA column (G-Biosciences, St. Louis, MO). Eluted protein was dialyzed against lysis buffer without imidazole and incubated with 1:20 mg of TEV protease at 4 °C for 20 h before being re-applied to the Ni-NTA column ...
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bioRxiv - Microbiology 2021Quote: ... Iodoacetamide (G-Biosciences, St. Louis, MO, or Sigma-Aldrich) was prepared as a 10 mM stock solution in HyPure H2O (Cytiva Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... Ni-NTA resin (G-Biosciences, St. Louis, MO, USA) was added to the supernatant after clarification by centrifugation at 15 k rpm at 4 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... magnetic silica beads (MGSBs, G-Biosciences, St. Louis, MO) were used to separate microsomal protein (Horspool et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and Protein G resin (G-Biosciences, St. Louis, MO, USA) prewashed with lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... and streptavidin agarose resin (G-Biosciences. St. Louis, MO. U.S.A) were washed according to the instructions and blocked by 1 mg mL-1 BSA at RT for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... after which Ni-NTA Resin (G-Biosciences, St. Louis, MO) was added to the solution ...
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bioRxiv - Genetics 2023Quote: ... coli prepared using Z-Competent cell kit (G-Biosciences, St. Louis, MO) and plated to achieve approximately 104 colonies per plate ...
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bioRxiv - Microbiology 2023Quote: ... purified proteins were mixed with alum adjuvant (G-Bioscience, St. Louis, MO) in a 2:1 ratio of antigen to adjuvant ...
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bioRxiv - Bioengineering 2020Quote: ... The Perforin ELISA kit was purchased from G-Biosciences (St. Louis, MO, USA). Melanoma cells (B16-F10 ...
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bioRxiv - Physiology 2022Quote: ... Total protein was assessed via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as the loading control ...
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bioRxiv - Physiology 2023Quote: ... Total protein was measured via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as a loading control ...
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bioRxiv - Genomics 2020Quote: ... Isolated colonies were lysed with Toothpick™-PCR (G-Biosciences, St. Louis, Missouri, USA), and supernatants were used as templates for ribotyping PCR using the following primers ...
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bioRxiv - Molecular Biology 2019Quote: ... and immersed in Coomassie stain (LabSafe GEL Blue; G-Biosciences; St. Louis, MO, USA) for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding was blocked with blocking buffer (G-Biosciences, St. Louis, MO, # 786195) for 1 hour and overnight incubation at 4°C with the rabbit pTau primary antibody (1:100 ...
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bioRxiv - Genetics 2019Quote: ... 60 and 61 using the OmniPrep™ kit (G-Biosciences, St. Louis, MO, U.S.A.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Digestion was done using proteomics grade trypsin 1:20 (G-Biosciences, St. Louis, MO) and a CEM Discover Microwave Reactor (Mathews ...
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bioRxiv - Pathology 2021Quote: Confluent EC monolayers were lysed in RIPA lysis buffer (G-Biosciences, St. Louis, MO) containing protease and phosphatase inhibitors (Boston BioProducts ...
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bioRxiv - Biochemistry 2023Quote: ... The gels were submerged under 1mg/ml sulfo-SANPAH (G-Biosciences, St. Louis, MO) solution and placed 2 inches below an 8 W ultraviolet UV lamp (Hitachi F8T5 – 365nm ...
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bioRxiv - Cancer Biology 2024Quote: ... protein concentrations measured using the Red 660 Protein Assay (G-Biosciences; St. Louis, MO), then lysate concentrations were normalized ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an OmniPrep Genomic DNA Extraction Kit (G-Biosciences, St. Louis, MO), following the protocol of Espeland et al ...
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bioRxiv - Plant Biology 2020Quote: ... Extracts were centrifuged and 25 μl of GST beads (G-Biosciences, St. Louis, MO USA) were added to 6 μl supernatant of GST-TPR2 and empty pGEX-4T-3 ...
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bioRxiv - Biochemistry 2022Quote: ... followed by incubation in developing buffer (30 min at RT, G-Biosciences, St. Louis, MO), and again in fresh developing buffer overnight at 37°C with gentle shaking ...
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bioRxiv - Cancer Biology 2019Quote: ... A non-radioactive colorimetric continuous enzyme kit (#786-430; G-Biosciences, St. Louis, MO, USA) was used to measure the enzyme activity of METTL18 according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... ELISA kits for rabbit TNFα and IL1β were from G-biosciences (St. Louis, MO, USA). A lipid peroxidase assay kit was from Eagle Biosciences (Amherst ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an Omniprep kit for genomic DNA (G-Biosciences, St. Louis, MO, USA), following the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... Roche/Sigma-Aldrich)] and protein concentration was determined using CB-X Protein Assay kit (G-Bioscience, St. Louis, MO). After trypsination ...
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bioRxiv - Biochemistry 2020Quote: ... Tris(hydroxymethyl)aminomethane HCl solutions at different pHs were purchased from G-Biosciences (St. Louis, MO). Trypsin Gold MS Grade was purchased from Promega (Madison ...
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bioRxiv - Molecular Biology 2019Quote: ... SPE Buffer I from the FOCUS™ SubCell kit (# 786-260, G-Biosciences, St Louis, MO) was added to the pellet ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genomic DNA was extracted using OmniPrep Genomic DNA Extraction Kits (G-Biosciences, St. Louis, MO, USA) and DNeasy Blood and Tissue Kits (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... 1% Tx100) supplemented with the protease inhibitor cocktail Protease Arrest (G-Biosciences, St. Louis, MO, USA). Antigen–antibody complexes were captured with Protein G-coupled Dynabeads (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Protein amounts were quantified using the CB-X™ protein assay (G-Biosciences, St Louis, MO). 1 mg of each sample was reduced at 37°C for 1 h and then alkylated with 20 mM iodoacetamide (20 min at RT in darkness) ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Omniprep DNA isolation kit (G-Biosciences, St. Louis, MO, USA) from 20 mg of urediniospores of Pca isolate NTU1 ...
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bioRxiv - Microbiology 2021Quote: ... After staining of the gels with Coomassie dye based RAPIDstain solution (G-Biosciences, St. Louis, MO, USA) MALDI-TOF-MS measurements were performed with an Ultraflex III TOF/TOF mass spectrometer (Bruker Daltonics ...
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bioRxiv - Genomics 2023Quote: ... we followed the kit standard protocol for Omniprep extraction of genomic DNA (G-Biosciences, St. Louis, MO) and sequenced 250 basepair paired-end reads on an Illumina MiSeq.
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bioRxiv - Synthetic Biology 2019Quote: ... The cells were plated in YPD agar plates containing 100µg/mL of Nourseothricin Sulfate (G-Biosciences, St. Louis, MO) and incubated at 30 °C for two days ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were collected and total protein was normalized using the Non-Interfering Protein Assay (G-Biosciences, St. Louis, MO).
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bioRxiv - Bioengineering 2022Quote: ... T7 RNA polymerase promoter was used for the overexpression of mutant protein by IPTG (G-Biosciences, St. Louis, USA) induction ...
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bioRxiv - Cancer Biology 2019Quote: ... The cytoplasmic proteins were also extracted from cells using the Nuclear & Cytoplasmic Extraction Kit (G-Biosciences, St. Louis, MO, USA). The proteins were resolved using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (10% ...
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bioRxiv - Biochemistry 2021Quote: ... The detergent present in the incubation mixture with BPS was removed using a DetergentOUT™ spin column (G-Biosciences, St Louis, MO). The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG ...
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bioRxiv - Genomics 2019Quote: ... Red blood cells were first removed with the addition of red blood cell (RBC) lysis buffer (G-Bioscience, St. Louis, MO, USA) and incubation for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.6 µL 1X TE Buffer (pH 8.0, Low EDTA (Tris-EDTA; 10 mM Tris base, 0.1 mM EDTA)) (PN 786-150, G-Biosciences, St. Louis, MO). Each dPCR reaction mix was then vortexed three times for five second pulses ...
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bioRxiv - Physiology 2021Quote: ... gels were rinsed in diH2O for 15 minutes and immersed in Coomassie stain (Lab Safe GEL Blue; G-Biosciences; St. Louis, MO, USA) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the cytotoxicity of extracts and pure compounds was determined according to the manufacturer's instructions using the CytoScan LDH cytotoxicity assay (G-Biosciences, St. Louis, MO). Briefly ...
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bioRxiv - Genomics 2021Quote: ... and pellets frozen at -80°C. DNA was extracted from pellets (approx. 100 mg) using an OmniPrep kit (G-Biosciences, St. Louis, MO) in 1 ml lysis buffer (G-Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, St. Louis, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 × g for 10 min) ...
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bioRxiv - Bioengineering 2020Quote: Actinobacillus succinogenes 130Z (ATCC 55618) was obtained from the American Type Culture Collection (Manassas, VA) and maintained in tryptic soy broth media (G-Biosciences, St. Louis, MO) with 10 g/L glucose (Fisher Chemical ...
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bioRxiv - Bioengineering 2023Quote: ... Protein concentration of EV samples was determined using a bicinchoninic acid (BCA) assay using the manufacturer’s protocol (785-571; G-Biosciences, St. Louis, MO, USA). Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429 ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from molecular collection specimens was extracted from the thorax or leg(s) using OmniPrep Genomic DNA Extraction Kits (G-Biosciences, St. Louis, MO, USA) or DNeasy Blood and Tissue Kits (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... All surfaces for tissue collection and processing were sanitized using 70% ethanol and then treated with an RNAse inhibitor (RNAse out, G-Biosciences, St. Louis, MO, USA) to maintain integrity of isolated RNA ...