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Citations for G-Biosciences :
1 - 50 of 109 citations for Saint Louis Encephalitis Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... 50 mM Tris-HCl pH 8) supplemented with PhosphataseArrest (G-Biosciences, Saint-Louis, MO, USA) and protease inhibitors (Complete Mini® ...
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bioRxiv - Bioengineering 2019Quote: ... A polyclonal anti-elastin antibody (custom-made at Clemson University) was thiolated with Traut’s reagent (G-Biosciences, Saint Louis, MO) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were detected by gene-specific 32P-labeled oligonucleotides (Supplementary Table S1) in hybridization buffer (G-Biosciences, Saint Louis, MI, USA) and exposed to a phosphor screen overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... protein concentrations measured using the Red 660 Protein Assay (G-Biosciences; St. Louis, MO), then lysate concentrations were normalized ...
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bioRxiv - Cell Biology 2020Quote: ... and Protein G resin (G-Biosciences, St. Louis, MO, USA) prewashed with lysis buffer ...
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bioRxiv - Physiology 2022Quote: ... Roche/Sigma-Aldrich)] and protein concentration was determined using CB-X Protein Assay kit (G-Bioscience, St. Louis, MO). After trypsination ...
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bioRxiv - Plant Biology 2023Quote: ... Protein amounts were quantified using the CB-X™ protein assay (G-Biosciences, St Louis, MO). 1 mg of each sample was reduced at 37°C for 1 h and then alkylated with 20 mM iodoacetamide (20 min at RT in darkness) ...
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bioRxiv - Microbiology 2023Quote: ... purified proteins were mixed with alum adjuvant (G-Bioscience, St. Louis, MO) in a 2:1 ratio of antigen to adjuvant ...
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bioRxiv - Physiology 2022Quote: ... Total protein was assessed via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as the loading control ...
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bioRxiv - Physiology 2023Quote: ... Total protein was measured via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as a loading control ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were collected and total protein was normalized using the Non-Interfering Protein Assay (G-Biosciences, St. Louis, MO).
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bioRxiv - Bioengineering 2022Quote: ... T7 RNA polymerase promoter was used for the overexpression of mutant protein by IPTG (G-Biosciences, St. Louis, USA) induction ...
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bioRxiv - Cancer Biology 2019Quote: ... The cytoplasmic proteins were also extracted from cells using the Nuclear & Cytoplasmic Extraction Kit (G-Biosciences, St. Louis, MO, USA). The proteins were resolved using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (10% ...
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bioRxiv - Microbiology 2021Quote: ... Iodoacetamide (G-Biosciences, St. Louis, MO, or Sigma-Aldrich) was prepared as a 10 mM stock solution in HyPure H2O (Cytiva Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... Ni-NTA resin (G-Biosciences, St. Louis, MO, USA) was added to the supernatant after clarification by centrifugation at 15 k rpm at 4 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... magnetic silica beads (MGSBs, G-Biosciences, St. Louis, MO) were used to separate microsomal protein (Horspool et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and streptavidin agarose resin (G-Biosciences. St. Louis, MO. U.S.A) were washed according to the instructions and blocked by 1 mg mL-1 BSA at RT for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... after which Ni-NTA Resin (G-Biosciences, St. Louis, MO) was added to the solution ...
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bioRxiv - Genetics 2023Quote: ... coli prepared using Z-Competent cell kit (G-Biosciences, St. Louis, MO) and plated to achieve approximately 104 colonies per plate ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Total protein staining was performed using Swift protein stain (G-Biosciences). Membranes were blocked in 5% milk in TBS- Tween for 1h prior to incubation with α-GFP (1:1000 ...
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bioRxiv - Bioengineering 2020Quote: ... The Perforin ELISA kit was purchased from G-Biosciences (St. Louis, MO, USA). Melanoma cells (B16-F10 ...
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bioRxiv - Microbiology 2021Quote: ... and protein concentration was determined using a noninterfering protein assay (G-Biosciences). 12 μg of protein was resolved by SDS-PAGE on a 17.5% acrylamide gel and transferred to an Immobilon-P membrane (Millipore) ...
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bioRxiv - Microbiology 2023Quote: Protein estimation was done using Bicinchoninic Acid (BCA) Protein Assay kit (G-Biosciences). Cell lysate (25ul aliquot of each replicate ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration was determined using a BCA Protein Assay kit (G-Biosciences) and subsequently treated with DNase I (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... Isolated colonies were lysed with Toothpick™-PCR (G-Biosciences, St. Louis, Missouri, USA), and supernatants were used as templates for ribotyping PCR using the following primers ...
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bioRxiv - Molecular Biology 2019Quote: ... and immersed in Coomassie stain (LabSafe GEL Blue; G-Biosciences; St. Louis, MO, USA) for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding was blocked with blocking buffer (G-Biosciences, St. Louis, MO, # 786195) for 1 hour and overnight incubation at 4°C with the rabbit pTau primary antibody (1:100 ...
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bioRxiv - Genetics 2019Quote: ... 60 and 61 using the OmniPrep™ kit (G-Biosciences, St. Louis, MO, U.S.A.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Digestion was done using proteomics grade trypsin 1:20 (G-Biosciences, St. Louis, MO) and a CEM Discover Microwave Reactor (Mathews ...
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bioRxiv - Pathology 2021Quote: Confluent EC monolayers were lysed in RIPA lysis buffer (G-Biosciences, St. Louis, MO) containing protease and phosphatase inhibitors (Boston BioProducts ...
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bioRxiv - Biochemistry 2023Quote: ... The gels were submerged under 1mg/ml sulfo-SANPAH (G-Biosciences, St. Louis, MO) solution and placed 2 inches below an 8 W ultraviolet UV lamp (Hitachi F8T5 – 365nm ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we assessed protein concentration in triplicate using RED 660™ protein assay (G-Biosciences). Following earlier tapeworm homogenate injection experiments (Vrtílek and Bolnick 2021 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an OmniPrep Genomic DNA Extraction Kit (G-Biosciences, St. Louis, MO), following the protocol of Espeland et al ...
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bioRxiv - Plant Biology 2020Quote: ... Extracts were centrifuged and 25 μl of GST beads (G-Biosciences, St. Louis, MO USA) were added to 6 μl supernatant of GST-TPR2 and empty pGEX-4T-3 ...
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bioRxiv - Biochemistry 2022Quote: ... followed by incubation in developing buffer (30 min at RT, G-Biosciences, St. Louis, MO), and again in fresh developing buffer overnight at 37°C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate was purified over a Ni-NTA column (G-Biosciences, St. Louis, MO). Eluted protein was dialyzed against lysis buffer without imidazole and incubated with 1:20 mg of TEV protease at 4 °C for 20 h before being re-applied to the Ni-NTA column ...
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bioRxiv - Cancer Biology 2019Quote: ... A non-radioactive colorimetric continuous enzyme kit (#786-430; G-Biosciences, St. Louis, MO, USA) was used to measure the enzyme activity of METTL18 according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... ELISA kits for rabbit TNFα and IL1β were from G-biosciences (St. Louis, MO, USA). A lipid peroxidase assay kit was from Eagle Biosciences (Amherst ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: ... Protein concentration of the samples was determined using the CB-X Protein assay (G-Biosciences) according manufacture’s guidelines ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an Omniprep kit for genomic DNA (G-Biosciences, St. Louis, MO, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Tris(hydroxymethyl)aminomethane HCl solutions at different pHs were purchased from G-Biosciences (St. Louis, MO). Trypsin Gold MS Grade was purchased from Promega (Madison ...
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bioRxiv - Molecular Biology 2019Quote: ... SPE Buffer I from the FOCUS™ SubCell kit (# 786-260, G-Biosciences, St Louis, MO) was added to the pellet ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genomic DNA was extracted using OmniPrep Genomic DNA Extraction Kits (G-Biosciences, St. Louis, MO, USA) and DNeasy Blood and Tissue Kits (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... 1% Tx100) supplemented with the protease inhibitor cocktail Protease Arrest (G-Biosciences, St. Louis, MO, USA). Antigen–antibody complexes were captured with Protein G-coupled Dynabeads (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Omniprep DNA isolation kit (G-Biosciences, St. Louis, MO, USA) from 20 mg of urediniospores of Pca isolate NTU1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Immobilized Protein G (cat# 786-284) and Protein A (cat# 786-283) resin from G-Biosciences were used at a 70:30 ratio ...
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bioRxiv - Biochemistry 2019Quote: ... The concentrations of these proteins were estimated by BCA protein estimation method (BCA assay kit, G-Biosciences). The in vitro ubiquitination assay was set up with the following components in the reaction mixture-5 nM Uba1p ...
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bioRxiv - Microbiology 2021Quote: ... After staining of the gels with Coomassie dye based RAPIDstain solution (G-Biosciences, St. Louis, MO, USA) MALDI-TOF-MS measurements were performed with an Ultraflex III TOF/TOF mass spectrometer (Bruker Daltonics ...