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Citations for SouthernBiotech :
1 - 50 of 374 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Slides were exposed to biotin labelled goat Fab anti-human IgG secondary antibody (Southern Biotech, Birmingham, AL) at a 1:2500 dilution for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... PE-labelled anti-human IgG (Southern Biotech, #2040-09) and biotin-labelled anti-Fab (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... IgA and IgG were detected by goat α-Human Fc IgA-Biotin conjugate and goat α-Human Fc IgG-Biotin conjugate (Southern Biotech, Birmingham, AL, USA) diluted 1:20,000 in PBS with 1% BSA and incubated for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... 3 µg/ml recombinant human TG2 or 2 µg/ml goat anti-human Ig (Southern Biotech) was coated in PBS followed by incubation with culture supernatants diluted in PBS supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... samples were labelled with biotin-coupled antibodies (all antibodies were purchased from Southern Biotech: goat anti mouse (1012-08), goat anti rat (3052-08) ...
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bioRxiv - Microbiology 2021Quote: ... but the secondary antibody used was an anti-human IgM Peroxidase-labelled antibody produced in goats (SouthernBiotech, Birmingham, USA).
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Immunology 2022Quote: ... with 0.5 μg Cy3B-labelled goat IgG1 anti-mouse Igκ (Southern Biotech; labelled in-house), 0.375 μg donkey anti-goat IgG (H+L ...
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bioRxiv - Immunology 2021Quote: ... and anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... anti-IgG1-biotin (Southern Biotech) and anti-IgG2a-PE ...
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bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
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bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
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bioRxiv - Neuroscience 2023Quote: ... biotin-conjugated secondary antibodies (1:200, SouthernBiotech) and avidin-biotin-peroxidase complex (Vectastain Elite ...
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bioRxiv - Immunology 2020Quote: ... and HRP-labelled anti-IgG1 antibodies (Southern Biotech) were used to detect plate-bound IgG1 ...
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bioRxiv - Immunology 2023Quote: ... Peroxidase labelled rat anti-mouse IgD (Southern Biotech), IgM (AbD Serotec ...
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bioRxiv - Bioengineering 2024Quote: ... Peroxidase-labelled anti-IgE antibody (#9160-05, Southern Biotech) was incubated (1/1000 ...
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bioRxiv - Physiology 2021Quote: ... A goat anti-mouse IgG- biotin antibody (Southern Biotech) was diluted 1:5000 in reagent diluent and added to all wells ...
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bioRxiv - Immunology 2023Quote: ... alkaline phosphatase (AP) labelled anti-Fc secondary antibody (Southern Biotech) at 1:2,000 in PBS/2% skimmed milk was added and incubated for 1 h ...
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bioRxiv - Immunology 2023Quote: ... alkaline phosphatase (AP) labelled anti-Fc secondary antibody (Southern Biotech) at 1:2,000 was added and plates were incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... and APC-labelled donkey anti-goat IgG (Southern Biotech, #6420-05) served as a secondary reagent ...
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bioRxiv - Immunology 2022Quote: ... Anti-mouse IgG and IgG1 conjugated to biotin (Southern Biotech), High Sensitivity Streptavidin-HRP (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... or anti-mouse IgA- biotin (Southern Biotech, Cat. #1040-08) and then streptavidin-HRP (Vector laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-mouse IgA-biotin (Catalog #1040-80, Southern Biotech). After a 1h incubation at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Rat anti-mouse IgG1-Biotin (SB77E) and Streptaviding-HRP (Southern Biotech) were used as detection and secondary antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100μL IgG-biotin HU adsorbed (Southern Biotech) for 2hr at RT ...
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bioRxiv - Immunology 2022Quote: ... Goat anti-mouse IgA biotin (Southern Biotech, 1: 5,000; AL, USA). Streptavidin-HRP (Vector Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08) diluted in PBS-BSA 1 % (100 μL/well) ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 hr at RT ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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bioRxiv - Immunology 2021Quote: ... a goat anti-mouse IgG biotin primary Ab (SouthernBiotech, Cat no. 1030-08) at (1:1000 ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
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bioRxiv - Immunology 2024Quote: ... or anti-human (SouthernBiotech) (1:5,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... IgA or IgM was detected with 30 mL of HRP secondary (Goat anti-human IgG HRP, Goat anti-human IgA HRP, Goat anti-human IgM HRP, all Southern biotech) at a 1:3000 dilution for 1 hour at 37C ...
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bioRxiv - Immunology 2020Quote: ... Goat-anti human IgA-PE or Mouse-anti human IgM-PE (Southern Biotech) and incubated on a plate shaker at RT for 2 h ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01 ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01, and anti-human IgG, SouthernBiotech, Cat # 2042-01) were conjugated on to selected peaks of Streptavidin coated 4um polystyrene capture beads ...
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bioRxiv - Immunology 2019Quote: ... A20 D1.3 cells were first incubated with biotin-HEL or biotinylated anti-IgM (Southern Biotech) for 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... while biotin-labeled goat anti-mouse IgA Ab (1:5000, Southern Biotech, Cat#1040-08) plus HRP-conjugated streptavidin (1:7000 ...
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bioRxiv - Microbiology 2024Quote: ... anti-human IgG and anti-human IgA secondary antibodies conjugated with Alkaline Phosphatase (Southern Biotech) were added for 45 min ...
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bioRxiv - Immunology 2023Quote: ... + Human IgM-L (cat# 0158L-01, southern biotech) + Human IgA-k (cat# 0155K-01, Southern Biotech). Goat anti-human IgM-AP (cat# 2020-04 ...
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bioRxiv - Immunology 2019Quote: A20 D1.3 cells were stained on ice for 10 min with anti-IgM-biotin (Southern Biotech) or HEL-biotin and washed with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 1/10,000 goat anti-mouse IgA-biotin (Southern Biotech, #1040-08) or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08 ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... goat anti-human Igκ (Southern Biotech), goat anti-mouse IgA (Southern Biotech) ...
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bioRxiv - Immunology 2022Quote: ... Anti-human IgG PE (Southern Biotech) was incubated at 0.65 μg/mL for 45 min in PBS-TBN ...