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Citations for SouthernBiotech :
1 - 50 of 698 citations for Mouse Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Model antigens used in solution or on PMS were goat anti-mouse Igκ F(ab’)2 (Southern Biotech) for mouse splenic B cells and goat F(ab’)2 anti-human Fc5μ (Jackson Immunoresearch ...
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bioRxiv - Immunology 2023Quote: ... and ELISAs were detected with HRP-conjugated goat-anti-mouse IgG1-HRP (Southern Biotech). To develop the enzymatic reaction TMB was used as described above ...
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bioRxiv - Immunology 2021Quote: ... Total antigen-specific IgG was then detected with a mouse anti-human IgG phycoerythrin-conjugated Ab (Southern Biotech) at 2 μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were coated with 2 µg/ml of goat anti-human IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... we used the following secondary antibodies during ELISA: goat anti-mouse IgG1 HRP antibody (#1071-05, SouthernBiotech), goat anti-mouse IgG2a HRP antibody (#1081-05 ...
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bioRxiv - Immunology 2021Quote: Goat F(ab’)2 anti-mouse Igκ (Southern Biotech) was biotinylated with 20-fold molar excess of NHS-LC-LC-biotin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab’)2 anti-mouse IgM (αIgM, SouthernBiotech) or rat anti-mouse CD40 (αCD40 ...
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bioRxiv - Immunology 2022Quote: Antigen-specific IgG (Cat. No. 1015-05, Southern Biotech, AL, USA), IgA (Cat ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Immunology 2021Quote: ... followed by goat F(ab’)2 anti-mouse IgG1-FITC (Southern Biotech). The percentage of living HUCCT1 cells was estimated by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
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bioRxiv - Cell Biology 2023Quote: ... goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech, Birmingham, AL); and anti-phospho-AKT (Ser473) ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Immunology 2021Quote: ... the same ELISA was performed but using the secondary antibodies from SBA Clonotyping System-HRP kit (SouthernBiotech, 5300-05, RRID:AB_2796080) diluted accordingly to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... or IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2020Quote: ... for detection of αGal and IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Zoology 2019Quote: ... and IgG3 levels of antigen-specific serum according to manufacturer instructions (Southern Biotech, Birmingham, AL, USA), and 96-well plates (Corning ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2020Quote: ... ELISAs were performed using alternate secondary antibodies (Sigma-Aldrich; Jackson ImmunoResearch; Southern Biotech). End point titers were extrapolated from sigmoidal 4PL (where X is log concentration ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Microbiology 2023Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... 500 ng of F(ab’)2 fragments of α-μ (clone JDC-15; Dako [α-human]; OB1022; Southern Biotech [α-mouse]) and α-CD19 (clone HD37 ...
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bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Immunology 2023Quote: ... and IgA concentrations were measured by ELISA using the SBA Clono-typing System (Southern Biotech).
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated secondary antibodies (anti-rabbit, mouse Ig, mouse IgG1, mouse IgG2a, and mouse IgG2b) were purchased from Southern Biotech. Alexa-conjugated secondary antibodies were from Life Technologies.
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Immunology 2022Quote: Serum from uninfected mice was analyzed for total IgG1 using a mouse clonotyping kit and standards (Southern Biotech) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... at the indicated timepoints serum was collected and analyzed by ELISA for anti-OVA antibodies (Southern Biotech).
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bioRxiv - Immunology 2020Quote: ... anti-mouse IgG1-HRP or anti-mouse IgG2a-HRP (Southern Biotech). After incubation and washes ...
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bioRxiv - Immunology 2024Quote: ... for mouse serum or mouse-anti-monkey IgG (SouthernBiotech; RRID: AB_2796069) for NHP serum) ...
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bioRxiv - Immunology 2023Quote: ... goat anti-mouse IgG2c or goat anti-mouse IgG3 (Southern Biotech). Plates were blocked for 1 hr using PBS 1% BSA ...
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bioRxiv - Genetics 2022Quote: ... Plates were washed using ELISA wash buffer and incubated with HRP-conjugated anti-Ig secondary antibodies (Southern Biotech) for 2 hours in humid storage at 4°C ...
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bioRxiv - Immunology 2023Quote: ... ELISA antibodies for measuring total and HDM-specific Igs in serum were purchased from Southern Biotech (Birmingham, AL). ELISA data were analyzed using SoftMax Pro software (Molecular Devices).
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bioRxiv - Immunology 2020Quote: ... anti-mouse IgG1 and anti-mouse IgG3 conjugated to HRP (Southern Biotech); TMB (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates were washed as described above and 100μl of secondary goat anti-rat IgG (SouthernBiotech Cat. 3030-04), goat anti-rat IgM (SouhternBiotech Cat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2023Quote: ... goat anti-mouse IgG2c-HRP or goat anti-mouse IgG3-HRP (Southern Biotech) and incubated for 1 hr at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the peptides was determined using a goat anti-human IgG HRP conjugate (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Immunology 2019Quote: ... Bound mouse IgG was detected with HRP-conjugated goat anti-mouse IgG (Southern Biotech), and the total IgG concentration was calculated with mouse IgG standards (Jackson ...
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bioRxiv - Immunology 2023Quote: ... antibody response to individual peptides was detected with different fluorescently labeled secondary antibodies (Goat anti-human IgM-AF488, SouthernBiotech, Cat # 2020-30 ...
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bioRxiv - Immunology 2022Quote: ... Purified mouse IgA (Southern biotech) was used in each plate for generating the standard curve.
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse-HRP (SouthernBiotech, catalog no 1030-05 ...
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bioRxiv - Immunology 2022Quote: ... Goat anti-mouse HRP (SouthernBiotech) 1:10000 was used for detection along with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher) ...