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Citations for SouthernBiotech :
1 - 49 of 49 citations for HBSS Solution since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed in 4% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Bioengineering 2023Quote: ... Cells were then fixed in 3.7% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL). Images were acquired using a Zeiss LSM 880 inverted confocal microscope (Carl Zeiss ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then washed x3 with HBSS and slides were mounted using Fluoromount-G (Southern Biotech). For intracellular immunostaining cells were washed x1 with PBS ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
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bioRxiv - Neuroscience 2020Quote: ... using Fluoromount-G solution (Southern Biotech #0100-01).
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bioRxiv - Genetics 2020Quote: ... stopped with a stopping solution (Southern Biotech, 0412-01), then read with a plate reader (Fisher Scientific accuSkan FC ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Slides were then mounted in fluoromount-G solution (SouthernBiotech) and pictures were taken either with a Laser confocal Zeiss LSM700 or Zeiss Axiovert 200M microscope.
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bioRxiv - Biophysics 2021Quote: ... We washed the cells with wash solution and added 50 µl of mounting solution (DAPI Fluoromount-G, 0100-20, Southern Biotech, Birmingham, AL). Finally ...
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bioRxiv - Immunology 2020Quote: ... plates were washed and incubated with ABTS peroxide substrate solution (SouthernBiotech) for 15 minutes or until color change occurred ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed and mounted using Fluoromount-G solution (SouthernBiotech).
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bioRxiv - Synthetic Biology 2024Quote: ... and mounted with fluoromount-G solution (Fluoromount- G, SouthernBiotech, #0100-01). Chips were incubated with Phalloidin at 0.66 µM (Alexa Fluor 488 ...
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bioRxiv - Immunology 2021Quote: ... and the reaction was stopped using TMB stop solution (SouthernBiotech, 0412-01) before reading absorbance at 450 nm.
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bioRxiv - Cancer Biology 2023Quote: ... and mounted in a DAPI-containing mounting solution (VectaShield or Southern Biotech). Primary antibodies used were guinea pig or rabbit anti-NG2 (Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... in blocking solution and mounted in Aqua-mount (Southern Biotech, 0100-01). Sections were counterstained with DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... sections were mounted in a mounting solution (fluoromount-G with DAPI, Southern Biotech). To confirm colocalization ...
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bioRxiv - Bioengineering 2019Quote: ... and treated with DAPI in the mounting solution Fluoromount-G (SouthernBiotech; 0100-20). Imaging was performed on a Nikon A1Rsi confocal microscope.
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bioRxiv - Immunology 2022Quote: ... Secondary detection antibody solutions of Mouse Anti-Human IgG Fc-HRP (SouthernBiotech, Birmingham, AL) diluted 1:10,000 in 1% milk PBST and Mouse Anti-Human IgA1-HRP/Mouse Anti-Human IgA2-HRP (SouthernBiotech ...
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bioRxiv - Physiology 2021Quote: ... the final wash was removed and pNPP one component microwell substrate solution (Southern Biotech) was added to each well ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed with PBS between treatments and finally mounted with Fluoromount solution containing DAPI (SouthernBiotech).
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were mounted onto glass coverslips using DAPI Fluoromount-G stain mounting solution (Southern Biotech).
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bioRxiv - Microbiology 2020Quote: ... After washing three times with PBS samples were mounted in Fluoromount G solution containing DAPI (Southern biotech) for DNA staining ...
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bioRxiv - Biophysics 2021Quote: ... stained their DNA using DAPI containing mounting solution (DAPI Fluoromount-G, 0100-20, Southern Biotech, Birmingham, AL), and imaged them using a wide-field fluorescence microscope ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed in PBS solution and incubated at room temperature in DAPI Fluoromount-G (Southern Biotech) before being mounted onto glass slides and coverslipped for imaging.
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bioRxiv - Microbiology 2023Quote: ... Sample homogeneous solution and standards (mouse IgA Isotype Control UNLB, Southern Biotech, Imtec Diagnostics, Ref: 0106-01) were diluted in Dilution Buffer (15 mM Trizma-Acetate ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μL of a secondary antibody solution (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Cell Biology 2021Quote: ... and coverslips were mounted on top of the cells using Fluoromount-G mounting solution containing DAPI (Southern Biotech). Single slice images were taken on a Zeiss 880 microscope using the confocal settings.
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bioRxiv - Biochemistry 2021Quote: ... and the secondary antibody solution was a 1:1200 dilution of goat-anti-human IgG Fc-HRP (SouthernBiotech). ELISA curves were fit to a 4-parameter logistic curve (Fig ...
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bioRxiv - Immunology 2020Quote: Model antigens used in solution or on PMS were goat anti-mouse Igκ F(ab’)2 (Southern Biotech) for mouse splenic B cells and goat F(ab’)2 anti-human Fc5μ (Jackson Immunoresearch ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was washed four times with PBS-T and 100 μL of TMB peroxidase substrate solution (SouthernBiotech, USA) was added to each well and incubated for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed three times in 1x PBS and covered in Fluoromount-G mounting solution (SouthernBiotech, Cat. 0100-01).
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bioRxiv - Immunology 2022Quote: ... The secondary antibody solution was prepared by diluting goat anti-human IgG HRP-conjugated antibodies (SouthernBiotech, cat. # 2040-05) at 1:2,000 in blocking buffer and adding 20 to 25 μL/well ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were mounted on the slide glass with a DAPI-containing mounting solution (DAPI Fluoromount-G, SouthernBiotech, USA). For Nkx2.1-2a-CreER::Ai14 and Nkx2.1-2a-CreER::Flex-FlpO mice immunohistochemistry ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and the samples were incubated with 50 μl of streptavidin-HRP diluted in blocking solution and standards with 50 μl of 0.175 μg/ml rat anti-mouse IgE-HRP (1130-05, Southern Biotech) diluted in blocking solution for 1 hour at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The staining procedure involved the following steps: (1) The sections were blocked in a solution of 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X in PBS for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were sectioned coronally at 50µm then brain slices were mounted on slide glasses and covered with fluoromount solution (SouthernBiotech, #0100-01) for imaging.
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bioRxiv - Molecular Biology 2019Quote: ... the coverslips were washed three times with 2X SSC buffer with 10% formamide and mounted onto coverslips using DAPI Fluoromount-G stain mounting solution (Southern Biotech). Cells were imaged and quantified as previously described (60).
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated at room temperature for 2 hours in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and then mounted using Hoechst-containing Fluoromount-G (Southern Biotech).
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bioRxiv - Neuroscience 2020Quote: One-third of the coronal sections near the target location were directly mounted from the cryostat and cover-slipped with a mounting solution (fluoromount-G with DAPI, Southern Biotech) to obtain accurate fiber location and to confirm virus expression without any staining ...
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bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Neuroscience 2023Quote: ... brains were sliced at 50µm slices using an Epredia HM430 microtome and the slices containing the target brain regions were mounted in DAPI solution (Product ID: 0100-20, Southern Biotech). Slices were imaged using fluorescence microscopy (Keyence BZ-X800) ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in fresh dilution solution with secondary antibody (goat anti-mouse IgG2a AlexaFluor 488, 1:250, Southern Biotech, cat# 1080-30) overnight at 4°C with gentle shaking ...
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bioRxiv - Immunology 2022Quote: ... was added to each well and incubated at RT for 3 minutes and the reaction was terminated with 50 μL of TMB Stop solution (SouthernBiotech, 0412-01). Absorbance at 450 nm (OD450 ...
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bioRxiv - Cancer Biology 2022Quote: ... the coverslips were washed 3 times with PBS and mounted on microscopy slides using FluoromountG solution containing DAPI (Southern Biotech; 0100-01). Slides were imaged using a Zeiss 880 LSM with Airyscan microscope.
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bioRxiv - Developmental Biology 2019Quote: ... A 1 μg/μl 4’6-diamidino-2-phenylindole (DAPI) solution was applied to the slides for nuclear staining prior to mounting in Fluoromount-G (Southern Biotech, 0100-01) with 34×60 mm coverslips (VWR ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed three times in PBS and once in MilliQ water before mounting with FluoromountG solution containing DAPI (Southern Biotech; 0100-01).
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bioRxiv - Cancer Biology 2023Quote: ... Then the cells were stained with DAPI solution and washed three times before mounting with Fluoromount-G® Anti-Fade Mounting Medium (Southern Biotech, Birmingham, AL). Images were captured using Zeiss Observer Z1 confocal microscope (Carl Zeiss ...