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Citations for SouthernBiotech :
1 - 50 of 608 citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... were detected with bromo-4-chloro-3-indolyl phosphate substrate (Southern Biotech).
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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bioRxiv - Biochemistry 2022Quote: ... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
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bioRxiv - Microbiology 2023Quote: ... and individual IgG subclasses 1-4 (SouthernBiotech) were diluted to manufacturer recommended concentrations and incubated for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Biochemistry 2023Quote: ... and 1/5,000 HRP-Steptavidin (100 μL/well) was added for 1 h (Southern Biotech, #7105-05). Plates were washed 4 times again with PBS-Tween 20 0.05 % ...
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bioRxiv - Immunology 2020Quote: ... or with goat anti-mouse IgM-HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT) for detection of M86 mAb ...
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bioRxiv - Neuroscience 2021Quote: ... for an additional 1 h and mounted in Fluoromount-G (Southern Biotech). For mice implanted with wire bundles ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit Anti-Sheep IgG(H+L)-AP (Southern biotech #6150.04, 1:2000) was used along with NBT (#S380C ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... coverslips were set in Fluoromount G medium containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (SouthernBiotech, Birmingham, Alabama) on microscope slides ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and then subsequently incubated for 1 h with 0.1 µg ml− 1 of anti-hamster IgG HRP (SouthernBiotech) in casein block at room temperature in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... dilution 1:10,000) and Goat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G ...
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bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
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bioRxiv - Microbiology 2022Quote: ... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
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bioRxiv - Cell Biology 2024Quote: ... Goat Anti-Mouse IgG(H+L) Human ads-HRP (Southern Biotech, 1:5,000), Goat Anti-Rabbit IgG(H+L ...
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bioRxiv - Cell Biology 2024Quote: ... Goat Anti-Rabbit IgG(H+L) Human ads-HRP (Southern Biotech, 1:5000), Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse/Human ads-HRP (4050-05, dilution 1:10000) and Goat Anti-Mouse IgG(H+L) Human ads-HRP (1031-05, dilution 1:10000) (Southern Biotech); sheep anti-TGN38 (AHR499G ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature and washed and mounted with Fluoromount-G (SouthernBiotech, #0100-01). For dendritic spine analysis ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... Goat Anti-Rabbit IgG(H+L) Mouse/Human ads-HRP (4050-05, dilution 1:10000) (Southern Biotech); Alexa 488- ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used include the following: Goat Anti-Mouse IgG (H+L; Southern Biotech, 1031-05, 1:2,000) and Goat Anti-Rabbit IgG (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... for C5b6 with a 1:5.000 of HRP-conjugated donkey antisera against goat IgG (H+L) (Southern Biotech) and for sMAC with 1:5,000 HRP-conjugated streptavidin (Southern Biotech) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Immunology 2023Quote: ... Membranes were then incubated with HRP-conjugated goat anti-rabbit IgG (H+L) (1:4000 dilution; Southern Biotech) at RT for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... and incubated for 1 h with mouse anti-rhesus IgG HRP-conjugated secondary antibody (Southern Biotech, clone SB108a) at a 1:5,000 dilution or anti-rhesus IgM HRP-conjugated secondary antibody (Rockland ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human IgG PE (SouthernBiotech, dilution 1:100), and PI (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... then incubated for 2 h at room temperature with Streptavidin-alkaline phosphatase from Southern Biotech at 2 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBST (1x, 5 min., RT) and incubated with goat anti-rabbit IgG- HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT). Membranes were washed with TBST (3x ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Molecular Biology 2020Quote: ... and then 4 × 1 hour prior to counterstaining with the nuclear marker (DAPI) (1 ng/ml) and mounting in Fluoromount G (Southern Biotech, Birmingham, AL). The primary antibody used was anti-Schistosoma japonicum Ago2 (clone 650-1-1-KM864-3-11E8 ...
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bioRxiv - Immunology 2021Quote: ... followed by the addition of horseradish peroxidase (HRP)-conjugated goat anti-Hamster IgG (H+L) (1:2000 dilution) (SouthernBiotech) for an hour at RT ...
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bioRxiv - Microbiology 2022Quote: ... a GAM IgG (H + L) conjugated to horseradish peroxidase was used as a secondary Ab (1:1000; Southern Biotech) and incubated with TBST (5% BSA ...
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bioRxiv - Microbiology 2022Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935–05, 1:8000 in PBST) or mouse anti-hamster-IgG1-HRP (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935-05, 1:8000 in PBST). Optical density was measured at 490 nm and 630 nm using an ELX800 plate reader (Biotek Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935-05, 1:8000 in PBST) or mouse anti-hamster-IgG1-HRP (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: ... Secondary staining was performed with a 1:10,000 dilution of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of 50 μL of 1:1000 anti-hamster-IgG(H+L)-HRP (Southern Biotech Cat. #6061-05) in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:10.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:5.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% BSA for 60 minutes at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed in PBS containing Tween-20 (0.05%) and was incubated for 1 h with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (Southern Biotech) diluted 1:20,000 accordingly ...
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bioRxiv - Cell Biology 2020Quote: ... and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech; 1-2 drops). Nuclear staining was detected with excitation at 405 nm ...
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bioRxiv - Immunology 2021Quote: ... After 3 washes in TBST the membrane was incubated with 1:10000 anti-mouse Ig (Southern Biotech) in TBST containing 5% non-fat dry milk for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... This will be followed by a 1 h incubation with biotinylated HRP conjugated goat anti-mouse IgG subtype antibodies (Southern Biotech). 3,3’,5,5’ tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 1 hr followed by the multi-species SP ads-HRP-conjugated goat anti-mouse IgG (H+L) antiserum (Southern Biotech) diluted at 1:20.000 and incubated at 37 °C for 1 hr ...