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Citations for Millipore Sigma :
1 - 50 of 7554 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The nucleotide sequences for RT-PCR and real-time PCR primers (synthesized by Sigma-Aldrich) are shown in Table 1 ...
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bioRxiv - Microbiology 2020Quote: For real-time quantitative reverse transcription PCR (RT-qPCR) total cellular RNA was extracted with Trizol (Sigma) or QiAmp (Qiagen ...
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bioRxiv - Microbiology 2022Quote: Quantitative real-time PCR (rt-qPCR) was carried out using the KAPA SYBR® FAST Mix (Sigma) on a LightCycler 480 (Roche Diagnostics ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ...
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bioRxiv - Cell Biology 2020Quote: ... the TRAPEZE® RT Telomerase Detection Kit (Chemicon; EMD Millipore) was utilized ...
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bioRxiv - Biochemistry 2023Quote: Mycoplasma PCR Detection Kit (LookOut, Sigma-Aldrich) Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Custom primers for real-time PCR were purchased from Sigma-Aldrich, Germany (GAPDH ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR-based method was used for detection of Mycoplasma with LookOut Mycoplasma PCR Detection Kit (Sigma) and JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR-based method was used for detection of Mycoplasma with LookOut Mycoplasma PCR Detection Kit (Sigma) and JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... the coated slide was washed 5 times with SSC 2X (Sigma) followed by 2 washes with dH2O ...
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bioRxiv - Cell Biology 2021Quote: ... and Sox2 cDNA were amplified by real-time qPCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich) and a 7500 Real-Time PCR Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... The LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) was used to assure that all cell lines were mycoplasma-free ...
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: ... SybrGreen Quantitative RT-PCR kit (Sigma Aldrich, Germany) was used with Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... and washed 3 times in 1x PBS and 2 times in 2x SSC buffer (Sigma-Aldrich) for five min each ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR using cDNA was performed using KAPA SYBR fast qPCR master mix kit (Sigma Aldrich, USA) and Applied Biosystems StepOne Plus Real-Time PCR system ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed using Jumpstart SYBR green mastermix (Sigma-Aldrich) on a Rotorgene 6000 (Corbett Research) ...
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bioRxiv - Cancer Biology 2020Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2019Quote: ... Real-time detection of miRNA expression was performed by using MystiCq miRNA qPCR Assay Primer (Sigma-Aldrich), using Universal Mouse Reference RNA and U6 as reference for mouse and human cells ...
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bioRxiv - Plant Biology 2019Quote: Q-PCR was performed on a Roche Lightcycler using standard reverse transcriptase kit and SYBR Green Real-Time PCR Master Mixes (SIGMA).
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bioRxiv - Cancer Biology 2021Quote: Gene expression was measured by quantitative RT-PCR and normalized to β-actin8,48 The SYBR Green quantitative RT-PCR kit (Sigma) was used with the following primers ...
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bioRxiv - Biochemistry 2019Quote: ... Venor GeM PCR-based mycoplasma detection kit was from Sigma. ECL substrate and ECL-Plus substrate were from Pierce and were used as directed ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were mycoplasma free (LookOut Mycoplasma PCR detection kit, Sigma). Cells were passaged with 0.05% trypsin-EDTA every 2-3 days ...
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bioRxiv - Immunology 2021Quote: ... as confirmed by the LookOut Mycoplasma PCR Detection Kit (Sigma). Cell lines were authenticated by the ATCC ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and real-time quantitative reverse transcriptase PCR analysis (qRT-PCR) was performed using SYBR Green® reagent (Sigma Aldrich) as previously described (46) ...
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bioRxiv - Genetics 2022Quote: ... Real-time PCRs were done in a 10 μl volume using the Absolute quantitative PCR SYBR Green mix (Sigma) in a 96-well plate ...
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bioRxiv - Cancer Biology 2023Quote: cDNA was diluted 10-50 times and quantified by real-time PCR with LightCycler® 480 SYBR Green I Master (SIGMA). The RPL11 ...
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bioRxiv - Microbiology 2023Quote: ... contamination (Lookout Mycoplasma PCR detection kit; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2021Quote: ... For gene expression analysis one-step qRT-PCR (SYBR Green Quantitative RT-PCR kit, Sigma) was carried out ...
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bioRxiv - Neuroscience 2020Quote: ... 2×SSC (Sigma), 10 % formamide (Calbiochem) ...
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bioRxiv - Developmental Biology 2019Quote: ... 5x SSC (Sigma), 5x Denhardt’s (from a 50x stock ...
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bioRxiv - Neuroscience 2023Quote: ... 2X SSC (Sigma), 0.2% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... along with mycoplasma testing using the LookOut Mycoplasma PCR Detection Kit (Sigma).
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bioRxiv - Immunology 2021Quote: ... and fluorescence imaging and LookOut® Mycoplasma PCR detection kit (Sigma-Aldrich). Primary antibodies used for immunohistochemistry included ...
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bioRxiv - Physiology 2022Quote: ... Each real-time PCR reaction mix was prepared with SYBR Green JumpStart Taq ReadyMix (Sigma, Oakville, Canada) and forward and reverse primer mix (3 µM ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed on Bio-Rad CFX96 real time (qPCR) system using predesigned KiCqstart primers (Sigma) and TBB green (#RR-208A ...
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bioRxiv - Bioengineering 2023Quote: ... then three times for 15 min in 5x SSC with 0.1 % Tween-20 (Sigma-Aldrich, P1379), and then incubated in HCR amplification buffer (2x SSC ...
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bioRxiv - Pathology 2019Quote: ... 2X SSC (Sigma, S6639)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 5X SSC (Sigma-Aldrich), 2% blocking powder (Boehringer ...
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bioRxiv - Cancer Biology 2022Quote: ... and 4X SSC (Sigma) at 37°C for 25 minutes each.
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed a further three times in TBS-T prior to antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was undertaken using a G:BOX Chemi-XR5 (Syngene ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three more times in TBS-T before antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was conducted using a G:Box Chemi-XR5 (Syngene ...
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bioRxiv - Molecular Biology 2019Quote: ... but tested negative for mycoplasma by PCR-based detection kit (Sigma-Aldrich D9307). Medium containing 0.5mM palmitate was prepared by first dissolving FFA-free BSA (Fisher Scientific BP9704100 ...
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bioRxiv - Pathology 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035). iPSC lines for the iMg were cultured by the Human Pluripotent Stem Cell Core (CHOP).
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bioRxiv - Immunology 2019Quote: ... Cells were verified as mycoplasma-free using the LookOut PCR detection Kit (Sigma).
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bioRxiv - Systems Biology 2020Quote: ... Mycoplasma was not detected with the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035).
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bioRxiv - Cell Biology 2021Quote: ... Mycoplasma contaminations were tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...