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Citations for Millipore Sigma :
1 - 50 of 2385 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ...
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bioRxiv - Physiology 2020Quote: ... The mouse RT-PCR primers (Sigma-Aldrich) used are shown in Supplementary Tab ...
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bioRxiv - Cancer Biology 2019Quote: ... The transfecting miR-590-5p mimics and inhibitors were obtained from Sigma-Aldrich, and the negative control (NC ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed utilizing the S-GAG set of primers and probe (Cline et al., 2005)(Sigma-Aldrich, Burlington, MA) with a 1-step Master mix (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Primer of miR-31 was ordered from Sigma (MIRAP00090) and RNU6-1 was used as the internal control (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... The nucleotide sequences for RT-PCR and real-time PCR primers (synthesized by Sigma-Aldrich) are shown in Table 1 ...
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bioRxiv - Bioengineering 2023Quote: ... clone 542-7D (MAB9013, Sigma-Aldrich) and against the SARS nucleocapsid protein (200-401-A50 ...
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bioRxiv - Immunology 2020Quote: ... Primers for Rnu6 and miR-21 were purchased from Sigma Aldrich. Primers for β-actin ...
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bioRxiv - Cancer Biology 2024Quote: ... Primer sets were purchased from Sigma-Aldrich’s KiCqStartTM line.
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bioRxiv - Cell Biology 2022Quote: ... 400nM RT primers (Sigma Aldrich)) and scRNA-seq primers ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Bioengineering 2019Quote: ... Primer probe sets were custom oligos (Sigma-Adrich) (Table 4).
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bioRxiv - Microbiology 2020Quote: ... The 3’UTR was determined by RT-PCR with an oligo-dT anchor primer (Sigma) as described58 with primers found in Table 4 ...
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bioRxiv - Microbiology 2020Quote: ... RT and PCR primers for the determination of the 3’UTR were obtained from Sigma. The remaining primers were purchased from TAG Copenhagen.
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bioRxiv - Cell Biology 2020Quote: ... miRNA-147-3p or miR-298-5p mimics (Dharmacon) and incubated for 72 hours in DMEM/OptiMEM medium (Sigma-Aldrich/Thermo Fisher Scientific) prior lysis.
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bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). For tissue conditioned media (TCM ...
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bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma).
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bioRxiv - Systems Biology 2019Quote: A mouse IgG primer set from Novagen (#69831-3) was used to amplify the IgG VH and VL regions from selected hybridoma clones ...
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bioRxiv - Bioengineering 2020Quote: ... All primer sets have been synthesized by Sigma-Aldrich DNA oligos design tool ...
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bioRxiv - Microbiology 2024Quote: ... with commercial RT-qPCR primers (KiCqStart SYBR Green Primers, Millipore Sigma KSPQ12012) and was assayed in Applied Biosystems QuantStudio 3 Real Time PCR System with standard cycling conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... clone 542-7D (Cat. MAB9012) was from Millipore Sigma; SARS-CoV-2/2019-nCoV Spike/S2 Antibody (Cat ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR primers (Sigma) were reconstituted in molecular grade water (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... All PCR primers (Sigma) are provided in Supplementary Table 1.
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bioRxiv - Cell Biology 2023Quote: ... and primer sets ordered from Sigma-Aldrich (Supplementary Table 2). Each master mix contained a 1X concentration of TB Green® Premix Ex Taq II (TLi RNaseH Plus) ...
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bioRxiv - Physiology 2019Quote: ... for synthesizing the probes was amplified using the primers described in Table S3 as following: the PCR reaction was set with KOD hot start master mix (#71842, Novagen), according to the manufacturer’s instructions and subsequently purified (#28706 ...
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bioRxiv - Synthetic Biology 2023Quote: ... which can be PCR amplified (Phanta Max Super-Fidelity DNA Polymerase, Vazyme) with a set of primers (ordered from Microsynth or Sigma-Aldrich) to add a unique variable linker ...
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bioRxiv - Neuroscience 2020Quote: ... Templates cDNA fragments for the synthesis of riboprobes were obtained by PCR using specific primer sets (Table 1) and subcloned into the plasmid pSTBlue-1 (Novagen, Madison, WI). Nucleotide sequencing revealed 100% homologies of these fragments to the reported sequences in Genbank ...
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bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ...
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bioRxiv - Developmental Biology 2022Quote: ... A probe for Nr2f2 was prepared by amplifying a cDNA fragment by RT-PCR (primers provided below, Table 4) from total RNA isolated from E9.5 embryos with Tri Reagent (Sigma #93289) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and universal PCR primer (Sigma-Aldrich) were used for RT-qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR primers (Sigma Aldrich, Taufkirchen, Germany) and probes (Roche Universal Probe Library ...
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bioRxiv - Immunology 2023Quote: ... and RT-PCR amplification data (Sigma). Samples were pooled and normalized to 10nM ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR primers were ordered as dried pellets from Sigma-Aldrich. The oligonucleotides were dissolved in 1X TE pH 8.0 to a final concentration of 100 μM and stored at 4 °C ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... PCR was performed using invadolysin primers (Sigma) designed to selectively amplify the invadolysin transgene ...
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bioRxiv - Cell Biology 2021Quote: ... PCR primers were purchased from Sigma-Aldrich. Zebrafish primer sequences have been previously published (Lim et al. ...
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bioRxiv - Microbiology 2019Quote: ... PCR primers were purchased from Sigma-Aldrich and are listed in S7 Table ...
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bioRxiv - Genomics 2021Quote: ... PCR primers were obtained from Sigma-Aldrich at 100 µM concentration in H2O ...
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bioRxiv - Immunology 2023Quote: ... and the following PCR primer sequences (Sigma): Gapdh (F ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR primers were purchased from Sigma, and the Biotin-TEG DNA antisense oligos were designed in Stellaris probe designer and purchased from IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... An aliquot (20 - 50 ng) of cDNA and a Forward/Reverse primer set (Sigma) were used in each PCR reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... except for a different set of SYBR green primers (Sigma Merck, Supplementary Table 2). The obtained Ct values were analyzed using the ddCt method for relative quantification of mRNA expression derived from three independent experiments ...
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bioRxiv - Neuroscience 2022Quote: ... DNA oligonucleotides (RT-qPCR primers) were obtained from Sigma-Aldrich (Rehovot, Israel). Mouse monoclonal antibodies against ARC (sc-17839 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 0.5μM PCR primers (Sigma-Aldrich; S4 Table) in a Real-Time PCR Detection System CFX96 (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Primer pairs for PCR were from Sigma (eGFP) and Invitrogen (GAPDH) ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequent PCR was performed with primers (Sigma-Aldrich) based on the Icelandic rock ptarmigan genome and Taq DNA polymerase (Qiagen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Nested PCR primers were ordered from Sigma-Aldrich’s custom oligo service ...
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bioRxiv - Cancer Biology 2021Quote: Gene expression was measured by quantitative RT-PCR and normalized to β-actin8,48 The SYBR Green quantitative RT-PCR kit (Sigma) was used with the following primers ...
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: ... SybrGreen Quantitative RT-PCR kit (Sigma Aldrich, Germany) was used with Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... The VH and VL sequences of B6 were recovered using the mouse iG primer set (Millipore) using the protocol outlined in (Siegel ...