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Citations for Millipore Sigma :
1 - 50 of 5562 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... HIV-1 PR cloning into the pET23a expression vector (Novagen), expression in Escherichia coli strain BL21 Star DE3 PlysS (Invitrogen) ...
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bioRxiv - Systems Biology 2021Quote: ... Competent cells were obtained using the Yeast Transformation Kit (Sigma YEAST-1KT).
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bioRxiv - Cell Biology 2021Quote: ... Yeast GLR1 was cloned into the first ORF of the expression vector pACYCDuet-1 (Novagen) including an N-terminal Flag-tag followed by a TEV cleavage site.
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bioRxiv - Microbiology 2019Quote: ... H-NS was purified by cloning the hns ORF into the pET28B expression vector (Novagen) to generate a 6xHis tag on the C-terminus ...
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bioRxiv - Synthetic Biology 2019Quote: ... was performed using the Yeast Transformation Kit (Sigma Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloning of it into NdeI-XhoI site of expression plasmid pET-22b(+) (Novagen, WI, USA).
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bioRxiv - Systems Biology 2021Quote: ... 600 μL of plate buffer (both from Sigma YEAST-1KT kit), 1 μg of P414 plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... Mad3 and Atf1 were generated by PCR of the corresponding gene fragments from yeast genomic DNA or cDNA and cloned in frame into expression vectors pET28a(+) (Novagen), pGEX-4T-1 (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Microbiology 2020Quote: ... yeast extract (Sigma) and agar were autoclaved in water ...
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bioRxiv - Cancer Biology 2021Quote: CDC20 cDNA was cloned into the multiple cloning site of the expression vector p3XFLAG-myc-CMV-26 (Sigma). Site-directed mutagenesis was used to generate the CDC20 mutants of interest ...
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bioRxiv - Microbiology 2022Quote: ... The resulting dsDNA libraries were cloned into the multiple cloning site of the pET-9a expression vector (Novagen) by Gibson assembly (NEBuilder HiFi DNA Assembly kit ...
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bioRxiv - Molecular Biology 2023Quote: ... mixed yeast tRNA and yeast Phenylalanine tRNA were obtained from Sigma for use in indicated assays ...
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bioRxiv - Genetics 2021Quote: ... and yeast extract (Sigma) M3+BPYE+10%FBS ...
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bioRxiv - Genomics 2019Quote: ... yeast extract (Sigma Y1000) and 10% FBS (Thermo Fisher Scientific 16000).
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bioRxiv - Microbiology 2019Quote: ... control yeast tRNA (Sigma) (100 ng or 300 ng ...
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bioRxiv - Bioengineering 2024Quote: ... yeast extract (Sigma Aldrich), bacto casitone (BD) ...
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bioRxiv - Immunology 2024Quote: ... or yeast RNA (Sigma), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... The cloning was performed according to the manufacturer’s protocol using the T7Select® 415-1 Cloning Kit (Millipore, 70015-3) with 1 μL of annealed oligonucleotides.
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bioRxiv - Cell Biology 2020Quote: KKT4 fragments used in this study were amplified from Trypanosoma brucei genomic DNA and cloned into the pNIC28-Bsa4 expression vector using ligation-independent cloning (Gileadi et al., 2008) or cloned into RSFDuet-1 vector (Novagen) using NEBuilder HiFi DNA Assembly Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The genes were inserted into the multiple cloning site 1 (MCS1) of the low copy expression vector pACYCDuetTM-1(Novagen), which was driven by a T7 promoter/lac operator with a chloramphenicol resistance gene for selection ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... aeruginosa SG17M genomic DNA was used as a template to amplify native PaftsH1 and PaftsH2 sequences for standard cloning into the expression vector pET-28a(+) (Novagen) between the NheI/XbaI restriction sites ...
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bioRxiv - Biochemistry 2022Quote: ... The YopH tyrosine phosphatase expression vector pESU009 was generated by cloning the YopH phosphatase domain (residues 177-468) into pACYCDuet-1 (Novagen) under transcriptional control of an attenuated tac promoter.37 All constructs were confirmed by DNA sequencing.
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bioRxiv - Biophysics 2023Quote: ... Plasmids were generated by custom DNA synthesis (Twist Bioscience, San Francisco, CA, USA) of the expression cassettes and cloning into the pRSFDuet-1 (Novagen) backbone between the PfoI and BshTI restriction sites ...
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bioRxiv - Cell Biology 2024Quote: ... Yeast cells were routinely grown on complete medium (Yeast peptone dextrose; YPD, Sigma) or synthetic complete (SC ...
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bioRxiv - Molecular Biology 2020Quote: ... A yeast protease inhibitor cocktail (Sigma-Aldrich; 50 μl per g of yeast cells) and dithiothreitol (DTT ...
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bioRxiv - Cancer Biology 2020Quote: ... PRL-3 over expressing construct was made by cloning full length PRL-3 cDNA into p3XFLAG-CMV-14 expression vector (Sigma, E7908)
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bioRxiv - Molecular Biology 2021Quote: ... or yeast tRNA (Sigma Aldrich) was set up in a reaction series that included mock (buffer) ...
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bioRxiv - Cancer Biology 2019Quote: ... Yeast Extract (Sigma-Aldrich; 70161), Peptone (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Yeast tRNAPhe (R4018, Sigma-Aldrich) spike-in control was added to each sample ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5% EZmixTM yeast extract (Sigma), and 1.125 g/L supplements mix containing equal amounts of adenine ...
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bioRxiv - Microbiology 2021Quote: ... 16mM Yeast Extract (BD, Sigma), 45mM NaCl (Fisher Chemical) ...
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bioRxiv - Microbiology 2020Quote: ... 3 g Yeast Extract (Sigma), 5 g D ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5% yeast extract (Sigma-Aldrich), 0.05% NaCl ...
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bioRxiv - Biophysics 2024Quote: The yeast tRNAPhe from Sigma was labeled on its dihydrouridine (D ...
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bioRxiv - Microbiology 2023Quote: ... active and inactive NS2B(H)-NS3 pro proteases were generated as described previously and cloned into the MCS1 (multiple cloning site 1) of dual protein expression plasmid pETDuet-1 (Novagen, Beijing, China) through restriction sites EcoRI and NotI ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was extracted from induced yeast cells using Spectrum Plant Total RNA Kit (Sigma-Aldrich) and cDNA was synthesised using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... We then packaged ligation reactions using T7Select415-1b cloning kit (Novagen (EMD Millipore), catalog #70015-3 ...
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bioRxiv - Bioengineering 2023Quote: ... We then packaged ligation reactions using T7Select415-1b cloning kit (Novagen (EMD Millipore), catalog #70015-3 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.7% Yeast nitrogen base (Sigma; #Y0626), 0.1% Complete synthetic medium (MP Biomedicals ...
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bioRxiv - Bioengineering 2022Quote: ... 10 g/L yeast extract (Sigma) and 10 g/L NaCl ((Fisher ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast autolysate was from Sigma-Aldrich, Sr ...
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bioRxiv - Developmental Biology 2022Quote: ... 50µg/ml yeast tRNA (Sigma, R6750)) at 70C with constant rocking for at least 2 hr before 1µg/ml DIG-labeled riboprobe was added and incubated overnight ...
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bioRxiv - Genetics 2020Quote: ... 1.48 % tRNA from baker’s yeast (Sigma), 1.15 mg/mL Proteinase K (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... and yeast (S. cerevisiae, Sigma-Aldrich) were used as phagocytosis targets ...
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bioRxiv - Genetics 2020Quote: ... 132mg/ml yeast RNA (Sigma, MO), 1μl RNAse inhibitor (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... 5 g Yeast Extract(Sigma-Aldrich), 10 g NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... 0.67% yeast nitrogen base (Sigma-Aldrich), and 2% agar and verified by PCR with yeast plasmid extraction (Solarbio Technology ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 0.5% yeast extract (Sigma) and cultured in the presence of 5% CO2 at 37°C or in Columbia agar supplemented with 5% horse blood (Oxoid ...
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bioRxiv - Microbiology 2023Quote: ... with 0.5% yeast extract (Sigma-Aldrich), and Brain-Heart Infusion media (Sigma-Aldrich) ...