Labshake search
Citations for Millipore Sigma :
1 - 50 of 10000+ citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... cells were cultured in media containing 50 μM biotin (Sigma) for 24 h ...
-
bioRxiv - Cell Biology 2019Quote: ... containing 50 µM Biotin (Sigma, B4501). After 18 h ...
-
bioRxiv - Neuroscience 2021Quote: ... APV (Sigma, WD 50 µM) were stored as recommended by supplier and diluted into aCSF to the working dilutions (WD ...
-
bioRxiv - Cell Biology 2020Quote: ... 150ml of PBS containing 6mM free biotin (Sigma) was added to the beads ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were treated with fresh media containing 50 μM D-biotin (Sigma B-4501). After 24 hours of labeling ...
-
bioRxiv - Microbiology 2022Quote: ... and effector reagents purchase from Millipore Sigma. BMDMs were generated and treated in XFe96 cell culture plates as described in the section “ex vivo experiments.” A combination of the Glycolysis Stress Test and Mito Stress Test was performed as previously described38 ...
-
bioRxiv - Neuroscience 2024Quote: ... Cell suspension was diluted to 2,500 live cells per ml and 35,000 cells were plated on glass coverslips or planar MEA coated with polylysine (Sigma, P-3143) and laminin ...
-
bioRxiv - Microbiology 2022Quote: ... 500nM of (GlcNAc)6 were added to trigger ROS production in the absence or presence of 1μM of effector protein in a measuring solution containing 100μM of L012 substrate (Sigma Aldrich) and 40μM horseradish peroxidase (Sigma Aldrich) ...
-
bioRxiv - Molecular Biology 2022Quote: ... mouse Cy3 anti-biotin antibody (#C5585 - Sigma) at 1:1000 ...
-
bioRxiv - Plant Biology 2019Quote: ... Pik-1 and AVR-Pik effectors were detected probing the membrane with anti-FLAG M2 antibody (SIGMA) and anti c-Myc monoclonal antibody (Santa Cruz) ...
-
bioRxiv - Plant Biology 2020Quote: ... Pik-1 and AVR-Pik effectors were detected probing the membrane with anti-FLAG M2 antibody (SIGMA) and anti-c-Myc monoclonal antibody (Santa Cruz) ...
-
bioRxiv - Biophysics 2022Quote: ... The primary antibody (1:100 Anti-Tau [3-repeat isoform RD3] Antibody, clone 8E6/C11, 05-803, Sigma-Aldrich and 1:100 Anti-Tau [4-repeat isoform RD4] Antibody ...
-
bioRxiv - Microbiology 2021Quote: ... The blocked cells were incubated with an α-HA-biotin primary antibody (Sigma-Aldrich) diluted 1:1,000 into PBS supplemented with 0.25% TX-100 for 30 min at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... one group of mice was switched from WD alone to WD supplemented with 2% cholestyramine (20g/kg of diet, Sigma) for 10 weeks (Supplementary Figure 1) ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-Tau 3-repeat isoform (RD3, Sigma), anti-Tau 4-repeat isoform (ET3 ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-Tau 4-repeat isoform (ET3, Sigma), anti-phosphorylated Tau (AT8 ...
-
bioRxiv - Cancer Biology 2022Quote: ... an NTP labeling mixture containing biotin-UTP (10 mM each ATP, CTP, and GTP, 6.5 mM UTP, 3.5 mM biotin-UTP; Sigma, 11685597910) was used for in vitro transcription.
-
bioRxiv - Cell Biology 2023Quote: Biotin-anti-6xHis antibodies (SIGMA, MA1-21315-BTIN) were flowed in through a streptavidin-coated flow channel ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were than incubated with primary antibodies raised against poly-glutamine repeats (5TF1-1C2, EMD millipore) or HTT (1HU-4C8 and mEM48 ...
-
bioRxiv - Neuroscience 2024Quote: ... The flow cells were incubated with 20 µg/ml anti-biotin antibody (Sigma Aldrich, B3640) in PBS for 10 min and passivated by 1% Pluronic F-127 (Sigma Aldrich ...
-
bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
-
bioRxiv - Molecular Biology 2023Quote: ... Target cells without effectors served as a negative control and target cells incubated with 2% Triton X-100 (Merck-Sigma) served as positive control (maximum killing).
-
bioRxiv - Bioengineering 2023Quote: Cells were incubated with 0.5mM biotin phenol solution (Biotin tyramide, Sigma Aldrich; SML-2135) for 15 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... Briefly, one day before transfection, effector cells (i.e., S-expressing cells) were seeded on the poly-L-lysine (Sigma, Cat# P4832) coated coverslips put in a 12-well plate ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
-
bioRxiv - Immunology 2020Quote: ... the medium was replaced by medium containing 40 μM of D-biotin (Sigma). The initial time-lapse acquisition characterizing TMEM176B was performed at Institut Curie (Paris ...
-
bioRxiv - Cancer Biology 2023Quote: Effector cells on coverslips were washed with phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde (PFA; Sigma) at room temperature (RT ...
-
bioRxiv - Genomics 2023Quote: ... Cells were authenticated by Short Tandem Repeat profiling (Genetica) and tested for mycoplasma (Sigma-Aldrich, MP0035). All cell lines were cultured in a humidified 5% CO2 incubator at 37 °C with media supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin (Life Technologies ...
-
bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
-
bioRxiv - Plant Biology 2019Quote: ... and a mouse anti-biotin monoclonal antibody (1:2,000; Sigma).
-
bioRxiv - Neuroscience 2023Quote: ... mouse anti-NeuN biotin conjugated antibody (1:2,000; MAB377B, Millipore), rabbit anti-parvalbumin (1:5,000 ...
-
bioRxiv - Plant Biology 2022Quote: ... resolved by SDS-PAGE and immunoblotted for the presence of the HA- tagged effector using α-HA antibody (H9658; Sigma-Aldrich) and α-HA-HRP (3F10 ...
-
bioRxiv - Molecular Biology 2023Quote: Proximity ligation assay using antibodies to RADX and an anti-biotin antibody to recognize EdU after conjugation to a biotin-azide were completed according to the manufacturer’s protocol (Sigma) and images obtained and quantified using a Molecular Devices ImageXpress instrument.
-
bioRxiv - Cell Biology 2019Quote: ... parasites were incubated 24 hours in media containing 2.5nM WR + 150μg of biotin (Sigma). Parasites were isolated using 0.04% saponin in 1X PBS and the lysates were analyzed via western blot as described above ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then biotinylated for 10-12 min in E4 containing 1.25 mg/mL NHS-SS-Biotin (Pierce) and quenched in E4 containing 20mM glycine (Sigma-Aldrich). Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III ...
-
bioRxiv - Cell Biology 2021Quote: ... Effectors for hydrogen sulfide synthesis used in experiments were cysteine (0.4mM; Sigma), JK-2 (300μM ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were biotinylated by incubation in biotin-free growth media supplemented with 50 µM biotin (Sigma-Aldrich). After exactly 20 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... SARS-CoV effector cells were washed twice with PBS and incubated with 3μg/ml of TPCK-treated trypsin (Sigma-Aldrich) for 30 mins at 37°C before resuspending in phenol red-free DMEM-10% ...
-
bioRxiv - Bioengineering 2023Quote: ... After selection for plasmid integration with 1 μg/ml puromycin for 5-7 days, cells were induced for effector (CasRx, DjCas13d or other Cas13d orthologs) expression using Doxycycline (D3072, Sigma). 3-5 days after induction ...
-
bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
-
bioRxiv - Biophysics 2020Quote: ... The pellet was then resuspended in conjugation buffer containing 1 mM Biotin-NHS (Sigma 203118) by pipetting up and down ...
-
bioRxiv - Cell Biology 2019Quote: ... cultures were incubated for 24 hours in media containing 2.5nM WR + 150μg of biotin (Sigma). 48 mL of asynchronous culture at high parasitemia (10% or higher ...
-
bioRxiv - Biochemistry 2021Quote: ... the media were replaced with fresh media containing 50 μM D-biotin (Sigma B-4501). After 16 hours of labeling ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were treated with biotin (50 μM; Sigma-Aldrich) for indicated exposure times ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated with 40 μM d-Biotin (Sigma) ingrown media for different time points (20 ...
-
bioRxiv - Cell Biology 2019Quote: ... sealed on the two planar faces by 22×22 mm glass coverslips welded by high vacuum grease (Sigma-Aldrich). Coverslips were previously acid-washed with a 20% HNO3solution for 2 hours at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... All cell lines were authenticated by Short Tandem Repeat profiling (Genetica) and routinely tested for mycoplasma (Sigma-Aldrich). All media for human cell lines were supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... and optimal analyte separation was achieved on a medium polarity SP2380 column (30 m × 0.25 mm × 0.20 μm, Sigma-Aldrich) with a constant helium flow of 0.8 mL/min and with oven temperature programmed to start at 55°C (hold 1 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... six repeats of CASTing selected binding sites synthesized by SIGMA-ALDRICH were introduced upstream of the TKmini promoter ...
-
bioRxiv - Cancer Biology 2022Quote: ... biotin (Sigma) was added to a final concentration of 50μM to transduced cells ...