Labshake search
Citations for Millipore Sigma :
1 - 50 of 1292 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
-
bioRxiv - Cancer Biology 2024Quote: ... Two different short hairpin (shRNA, from Sigma-Aldrich shRNA library; Table 1), scrambled shRNA control (Addgene plasmid #1864) ...
-
bioRxiv - Cell Biology 2023Quote: ... elegans cDNA library were ligated into pET23 (Novagen) via Gibson assembly ...
-
bioRxiv - Cancer Biology 2023Quote: ... DNA-RNA hybrid (Sigma-Aldrich, MABE1095), and anti-DNA PKcs (phospho S2056 ...
-
bioRxiv - Cell Biology 2020Quote: ... full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 cytoplasmic tail (aa 568-976 ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA library preparation and amplification were performed with WTA2 kit (Sigma-Aldrich) using 2-5 ng of total RNA as template ...
-
bioRxiv - Molecular Biology 2020Quote: Antibodies: anti-DNA-RNA hybrid S9.6 (Millipore, MABE1095), HA (Santa Cruz ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Dimethyl sulfoxide (DMSO) hybrid-max sterile (Sigma, D2650) was diluted in fish water to a final concentration of 0.1% (v/v) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The cDNA and libraries were then quantified using KAPA Biosystems qPCR (Sigma-Aldrich) and sequenced using Novaseq6000 (Illumina ...
-
bioRxiv - Microbiology 2023Quote: Two independent guide RNAs for ITGA5 (Table S2) from the Brunello sgRNA library (49) were synthesized (Sigma- Aldrich) and cloned in to the pLentiCRISPRv2-blast plasmid (#98293 ...
-
bioRxiv - Immunology 2023Quote: ... a targeted amplicon library was generated by PCR from the standard gene expression library cDNA using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004). 50uL PCR reactions were prepared by combining 25uL ReadyMix with 2.5 uL of a 10uM stock of each primer (10x Partial Read 1 ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA-DNA Hybrid clone S9.6 (1:200; MABE1095; Millipore), CENP-C (1:1000 ...
-
bioRxiv - Cell Biology 2019Quote: ... α-DNA-RNA hybrid (S9.6) antibody is from Sigma Aldrich.
-
bioRxiv - Molecular Biology 2021Quote: ... anti-DNA:RNA hybrid clone S9.6 (mouse, MABE1095 [Merck Millipore] ...
-
bioRxiv - Molecular Biology 2022Quote: ... Anti-DNA-RNA hybrid antibody S9.6 (MABE1095, Sigma-Aldrich), anti-NRBP1/2 antibody (21549-1-AP ...
-
bioRxiv - Microbiology 2023Quote: ... 2% 50X HT Media Supplement Hybrid-MaxTM (Sigma, H0137), 1% 200 mM L-Glutamine (Gibco ...
-
bioRxiv - Microbiology 2024Quote: ... 2% 50X HT Media Supplement Hybrid-MaxTM (Sigma, H0137), 1% 200 mM L-Glutamine (Gibco ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNAs were purified by two rounds of immunoprecipitation with Dynabeads protein G conjugated to anti-BrdU (clone IIB5, Millipore). The purified cDNAs were then ligated using CircLigase II (Lucigen ...
-
bioRxiv - Genomics 2021Quote: We extend our TWO-SIGMA method [38] to competitive gene set testing via TWO-SIGMA-Geneset (TWO-SIGMA-G), on overview of which is shown in Figure 1 ...
-
bioRxiv - Cell Biology 2020Quote: ... Semaphorin 6B cytoplasmic tail (aa 616-1021) (SEMA6B, amplified from human brain cDNA library, Novagen); Semaphorin 6D cytoplasmic tail (aa 684-1073 ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA libraries were quantified and normalized by using the KAPA SYBR Fast Mastermix (Sigma-Aldrich) with P5-P7 Illumina primers according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... two μg of RNA were utilized for cDNA synthesis using MMLV-reverse transcriptase and oligo-dT (Sigma: M 1302 and O4387, respectively). A CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad ...
-
bioRxiv - Genomics 2023Quote: ... for non-hybrid crosses covered with mineral oil (Sigma Cat# M8410) at 37°C with 5% CO2 and 5% O2 air.
-
bioRxiv - Neuroscience 2020Quote: ... Two mM of ANS (Sigma) stock solution was prepared in PBS pH 7.4 ...
-
bioRxiv - Cell Biology 2020Quote: ... Two-mM thymidine (Sigma-Aldrich) was applied to the culture medium for 12 hours to arrest the cells at the stage of G1/S phase transition ...
-
bioRxiv - Plant Biology 2021Quote: ... Two pairs of oligos (Sigma) corresponding to the consensus gRNA target site in exon two of MYB186 (Potri.008G089200) ...
-
bioRxiv - Neuroscience 2023Quote: ... two DiI coated (Sigma-Aldrich) laminar 32 channel probes (H4 ...
-
bioRxiv - Genomics 2023Quote: Two different linkers (Sigma-Aldrich), a 5’ linker and a 3’ linker ...
-
bioRxiv - Genetics 2023Quote: ... two oligonucleotides were synthesized (Sigma), annealed and cloned into a CRISPR-Cas9 expression vector (pX330-hCas9-long-chimeric-grna-g2p ...
-
bioRxiv - Physiology 2024Quote: ... connected to two current and two voltage electrodes filled with 1.5% (w/v) agar (Millipore) dissolved in 3M KCl ...
-
bioRxiv - Neuroscience 2020Quote: ... two-trial analog training was delivered by two pulses of 5HT (50 μM, Sigma, #H9523-25MG) in ASW (ITI = 45 min) ...
-
bioRxiv - Cell Biology 2021Quote: ... with two vectors from Sigma-Aldrich, targeting the two stress-inducible Hsp70 variants Hspa1a (Gene ID ...
-
bioRxiv - Molecular Biology 2021Quote: ... to two different siRNAs (Sigma Millipore) (start on target sequence CDS position 2393bp-sense ...
-
bioRxiv - Molecular Biology 2021Quote: ... to two different siRNAs (Sigma Millipore) (start on target sequence CDS position 2393bp-sense ...
-
bioRxiv - Neuroscience 2021Quote: ... two from Sigma-Aldrich (Poole, UK) and one from Amersham ...
-
bioRxiv - Immunology 2020Quote: ... Two units of biotinylated thrombin (Millipore) were mixed with each 1 mg of protein overnight to cleave off tags ...
-
bioRxiv - Neuroscience 2021Quote: ... Two μl of 6-OHDA (Sigma) (5 μg/μl ...
-
bioRxiv - Cell Biology 2022Quote: ... to transfect two siRNAs (Sigma-Aldrich) for each target mRNA ...
-
bioRxiv - Cell Biology 2022Quote: ... two conditions of ML323 (Sigma Aldrich) treatment were maintained ...
-
bioRxiv - Biophysics 2023Quote: ... We used two ligands hemin (Sigma) and AMP.PNP ...
-
bioRxiv - Molecular Biology 2021Quote: ... The captured DNA-RNA hybrids were enriched by protein A-agarose beads (Millipore). The purified DNA was used to construct a library with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
-
bioRxiv - Biophysics 2019Quote: Mouse neuroblastoma x rat neuron hybrid ND7/23 cells (ECACC 92090903, Sigma Aldrich) were grown in high glucose Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Neuroscience 2022Quote: Mouse neuroblastoma x rat neuron hybrid ND7/23 cells (ECACC 92090903, Sigma Aldrich) were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Cancer Biology 2020Quote: ... S9.6 antibody that binds to RNA/DNA hybrid was purchased from Millipore (MABE1095).
-
bioRxiv - Bioengineering 2020Quote: ... This was achieved using Red Blood Lysis buffer (Sigma-Aldrich, hybrid-max™) which preferentially lyses RBCs from a mixed population of cells ...
-
bioRxiv - Biochemistry 2021Quote: The hnRNP L_RRM2 fragment (residues189-286) was amplified from the human brain cDNA library and cloned into a modified pET28a (Novagen) vector without a thrombin protease cleavage site (termed p28a) ...
-
bioRxiv - Genomics 2023Quote: Injected zygotes were cultured in KSOM for hybrid crosses (Sigma, Cat#MR-106-D) or M16 medium (Sigma ...
-
bioRxiv - Genomics 2019Quote: ... treatment and two sequential phenol/chloroform (Sigma) extractions ...
-
bioRxiv - Immunology 2021Quote: ... Two complementary oligos were synthesised (Sigma Aldrich) and combined at equal molar ratio ...
-
bioRxiv - Plant Biology 2021Quote: ... filtered through two layers of Miracloth (Millipore) and pelleted by centrifugation at 4 °C for 15 min at 3600 g ...