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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Thiol Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Thiol concentration of culture supernatants was measured using a fluorometric thiol assay kit according to the manufacturer’s instructions (Sigma-Aldrich, MAK151). Fluorescence was measured with a BioTek Synergy H1 microplate reader (Agilent ...
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bioRxiv - Bioengineering 2019Quote: ... sericata maggot E/S was assessed using a Protease Fluorescent Detection Kit (Sigma Aldrich), This kit detects the activity of serine ...
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bioRxiv - Evolutionary Biology 2020Quote: ... called Thiol Probe IV (EMD Millipore) to make a fluorescent glutathione conjugate ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was recovered and used with the protease fluorescent detection kit (PF0100 – Sigma-Aldrich). The protocol was adjusted from the manufacturers’ instructions in the following ways ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescent signal was generated using Duolink In Situ Detection Reagents Red kit (Sigma-Aldrich, DUO92008). Cells were mounted and the nuclei were stained using the Duolink In Situ Mounting Medium with DAPI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... BACE1 activity was measured using the β-Secretase (BACE1) Activity Detection Kit (Fluorescent) (Sigma: CS0010). Equal amounts of lysate were added to each well and run in duplicate ...
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bioRxiv - Microbiology 2019Quote: ... thiols were alkylated in 5 mM iodacetamide (Sigma) for 15 min at 25 °C in the dark ...
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bioRxiv - Systems Biology 2021Quote: ... at a 0.99:1 thiol-to-ene ratio in phosphate buffered saline (PBS, Sigma-Aldrich). Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP ...
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bioRxiv - Cancer Biology 2020Quote: ... Red fluorescent signals was obtained using detection reagent red (Sigma-Aldrich: DUO92008) and chromogen signals was acquired using detection reagent brightfield (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: Ethanol-activated low fluorescent PVDF 96-well plates (Millipore) were coated with monoclonal antibodies to bovine IFNγ and IL-2 (MABTECH) ...
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... 450 µM Thiol-dPEG®4-acid (Sigma-Aldrich QBD10247) and 250 µM TCEP-HCl (tris(2-carboxyethyl)phosphine-HCl ...
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bioRxiv - Developmental Biology 2020Quote: The cell death detection kit (In Situ Cell Death Detection Kit, Sigma-Aldrich) was used to perform TUNEL analysis according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 20 μl of 1 g/ml methoxy PEG thiol (average Mn ~800; Sigma-Aldrich, cat #729108). Methoxy PEG thiol was used to mask unbound maleimide groups and to prevent aggregation of spheres while demulsifying ...
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bioRxiv - Cancer Biology 2023Quote: ... fluorescent cell linker midi kit (Sigma) was used according to manufacturer instructions to label murine mammary carcinoma cell lines ...
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bioRxiv - Bioengineering 2022Quote: ... thiol-PEG-acid (HOOC-PEG-SH; PEG average Mn 5,000; Sigma), poly(ethylene glycol ...
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bioRxiv - Bioengineering 2021Quote: Thiol-modified gelatin (GelSH; Lot: MKCJ5413) was obtained from Sigma Aldrich and used as provided ...
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bioRxiv - Physiology 2021Quote: ... ROS detection kit (D6883, Sigma) containing 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and chemiluminescence detection kit (Millipore) were used for visualization with ChemiDoc Touch Imaging System (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... TLC plates (Silica gel 60, non-fluorescent, 0.25mm thick, Merck Millipore) were cut to a width that allowed ∼1cm between each lipid sample ...
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bioRxiv - Developmental Biology 2022Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Maleimide-based functionalization of thiol-modified siRNAs (custom-ordered from Millipore-Sigma) was accomplished in PBS + 5 mM TCEP at pH 7.3 ...
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bioRxiv - Molecular Biology 2024Quote: ... Maleimide-based functionalization of thiol-modified siRNAs (custom-ordered from Millipore-Sigma) was accomplished in PBS + 5 mM TCEP at pH 7.3 ...
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bioRxiv - Neuroscience 2020Quote: ... Chemiluminescence was generated by ImmunoStar detection kit (Wako) or Immobilon (Millipore) and detected by LAS-3000 mini system (FUJIFILM).
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bioRxiv - Genetics 2020Quote: Cell Growth Detection Kit (MTT) (Sigma) was used for detecting cell proliferation ...
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bioRxiv - Neuroscience 2022Quote: ... followed by detection using standard ECL chemi-luminescence detection kit (Millipore, WBKLS0500).
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bioRxiv - Microbiology 2024Quote: ... Signal detection was carried with DIG luminescence detection kit (Sigma Aldrich, #11363514910) following manufacturers instruction and signal captured with ChemiDoc (Biorad) ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescent-conjugated phalloidin (1:1000; Sigma) and DAPI (1:10000 ...
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bioRxiv - Microbiology 2020Quote: ... USA) or the fluorescent signal of each protein was measured with a chemiluminescence detection system (Millipore) using Scion Image software (Scion Corp. ...
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bioRxiv - Immunology 2022Quote: ... Low fluorescent 96-well polyvinylidene fluoride sterile plates (Millipore, Bedford, MA, USA) were pre-wetted with 20 µL/well of freshly prepared 40% ethanol solution for one minute ...
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bioRxiv - Cell Biology 2020Quote: ... free thiols were blocked by addition of 10 mM N-ethyl maleimide (Sigma) and incubated on a rotator at 4°C for 1 hour.
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bioRxiv - Biochemistry 2021Quote: ... and alkylation of thiols was performed with 55 mmol/L iodoacetamide (Sigma-Aldrich) in 8 mol/L urea in 100 mM Tris-HCl (pH 8.5) ...
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bioRxiv - Bioengineering 2019Quote: For further functionalization experiments thiol-PEG-amine 2000 Da (SH-PEG-NH2) (Sigma) was used as a platform for bioorthogonal click chemistry ...
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bioRxiv - Biochemistry 2024Quote: ... we follow the Protocol for Thiol-Modified Aptamers DDT Reduction from Sigma Aldrich and Cytiva and the Conjugation Protocol of Maleimide Gold Nanoparticles with Aptamers from Cytodiagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... or DMSO plate seeded with OP50 containing a 1:100 dilution of red fluorescent latex bead solution (Sigma #L3280) and shifted to 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by alkylation of cysteine residue side chain thiol groups with iodoacetamide (Sigma-Aldrich) at approximately 17 mM final concentration for 30 min at room temperature in the dark ...
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bioRxiv - Biophysics 2022Quote: ... 1 μl of 1 μm fluorescent beads (Sigma, L9654) were added along with 15 μl 10% APS and TEMED and 10 μl TEMED to achieve a final bead concentration of 5 × 1010/ml ...
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bioRxiv - Bioengineering 2023Quote: ... and 1% fluorescent beads (1:500, Sigma Lot L3030) that were used for drift-compensation within a glass capillary ...
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bioRxiv - Immunology 2023Quote: ... quantified using LDH Cytotoxicity Detection Kit (Sigma) according to manufacturer directions ...
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bioRxiv - Biochemistry 2023Quote: Mycoplasma PCR Detection Kit (LookOut, Sigma-Aldrich) Colloidal Blue Staining Kit (Invitrogen ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: ... Single-stranded DNA oligonucleotides with amine or thiol functional groups were purchased from Sigma. Before annealing the DNA scaffold ...
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bioRxiv - Physiology 2020Quote: ... the newly reduced thiols were labeled by incubation with 5 mM mBBr (Merk Millipore) in sample buffer during 2 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was subjected to thiol(SH)-linked alkylation by iodoacetamide (Sigma, 10 mM) at 500C for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and free thiols were alkylated with 40 mM 2-chloroacetamide (Sigma, 22790-250G-F) in 100 mM Tris pH 8.0 ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... The non-oxidized molecules were removed by Activated Thiol SepharoseTM 4B chromatography (Sigma Aldrich) performed according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and biotinylated detection antibody was added before the plates were incubated with Streptavidin peroxidase (1:1,000, Sigma) for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... All wells were treated with the fluorescent detection probes 2’,7’-dichlorofluorescein diacetate (10µM, DCF-DA; Sigma, D2926) or 4,5-diaminoflurescein diacetate (5µM ...
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Cell Biology 2022Quote: ... Detection of the hybridized antibody was performed using an ECL detection kit (Immobilon Western, Millipore), and the signal was visualized with a digital camera (ChemiDoc XRS+ ...