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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... anti-gamma-tubulin from Sigma (T-6557). Secondary HRP-conjugated antibodies were from Bio-Rad ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... t Phospho-Myosin Light Chain 2 (Ser19) antibody (3671; Cell Signalling) 1:50 and Recoverin antibody (AB5585; Millipore) 1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... dilution 1:10) cell-surface antibodies after a non-specific sites’ saturation step with Gamma Immune (Sigma Aldrich, dilution 1:2). Isotype controls (Miltenyi Biotech) ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-rabbit IgG (gamma-chain specific, Sigma, #A1949-1VL; 1:4,000) antibodies.
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bioRxiv - Microbiology 2021Quote: ... Gamma-delta T cells (γδ T cells) were separated from heparinised peripheral blood by density-gradient centrifugation with Histopaque-1077 (Sigma, Spain). Phagocytosis assay was carried out in heparinized whole blood samples incubated with fluorescein (FITC)-labelled opsonized Escherichia coli bacteria using the Phagotest kit (BD Pharmigen ...
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bioRxiv - Cell Biology 2023Quote: ... gamma-tubulin rabbit antibody (Sigma Aldrich), Pericentrin rabbit anibody (Abcam) ...
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bioRxiv - Developmental Biology 2022Quote: ... MAG (Anti-Myelin Associated Glycoprotein, clone 513 antibody, Millipore mab1567 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transferred to ice and surface biotin was cleaved with 60 mM MesNa (Sigma, 63705) in MesNa buffer (50 mM Tris-HCl [pH 8.6] ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit polyclonal gamma tubulin antibody (Sigma, Cat#-T5192). Goat polyclonal ankyrin-G antibody (Paez-Gonzalez et al. ...
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bioRxiv - Immunology 2022Quote: ... human CD3+ T cells were stimulated with 10ng/ml PMA and 1μM Ionomycin (Sigma-Aldrich) or with 1 μg/mL pre-coated α-CD3 and 1 μg/mL soluble α-CD28 ...
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bioRxiv - Biophysics 2021Quote: ... DNA-fluorophore constructs were immobilized on the surface using biotin-avidin as follows: BSA-biotin (A8549, Sigma-Aldrich) was dissolved and diluted in buffer A (10 mM Tris ...
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bioRxiv - Biophysics 2022Quote: GMPCPP-stabilized microtubule seeds polymerized from biotinylated and TAMRA-labeled bovine tubulin were attached to the surface of a microchannel using anti-biotin antibodies (Sigma-Aldrich). Microtubules were assembled using 12 μM unlabeled bovine GTP-tubulin for 10 min at 28 °C in polymerization buffer (80 mM K-PIPES ...
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bioRxiv - Biophysics 2024Quote: ... We used the docking strand R4* modified with biotin on its 5’ end and immobilized it onto coverslip surface functionalized with BSA-biotin (A8549, Sigma-Aldrich) and then Neutravidin (31000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The Antibody against gamma-tubulin (T5326) was purchased from Sigma.
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bioRxiv - Immunology 2023Quote: ... Monoclonal antibodies were produced in 50 ml of 0.8×106 HEK293-6E cells by co-transfection of heavy chain (IgG1) and light chain (Igλ, Igκ) antibody expression plasmids with polyethylenimine (PEI; Sigma-Aldrich). Cells were maintained at 37°C and 5% CO2 in FreeStyle 293 Expression Medium (Thermo-Fisher ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-CD3 antibody (Sigma, SAB5500057) diluted 1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining biotin on the cell surface was stripped with 50 mM of sodium 2-mercaptoethanesulphonate (MESNa) (M1511, Sigma-Aldrich) in PBS/CM at 4°C for 30 min followed by quenching with 5 mg/ml iodoacetamide (093-02152 ...
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bioRxiv - Cell Biology 2019Quote: ... and a monoclonal antibody against gamma-tubulin 1:1000 (T5326, Sigma) prior to incubation with appropriate secondary antibodies conjugated to Alexa Fluor 488 (A11017 ...
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bioRxiv - Genetics 2022Quote: ... and mouse monoclonal gamma tubulin primary antibodies (T6557, Sigma, Taufkirchen, Germany), overnight at 4°C after permeabilizing with 0.5% Triton-X 100 ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin-647-microtubules were attached to this surface with streptavidin (0.625 mg/ml) (#S4762 Sigma) and the surface was blocked with κ-casein (1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
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bioRxiv - Immunology 2020Quote: Anti-CD3 antibody was from Sigma-Aldrich, St ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Mouse monoclonal antibody MAB8150 against WNV E glycoprotein was from EMD Millipore (Billenca MA). Alexa Fluor 488 goat anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells isolated from healthy donor PBMCs were stimulated by a 1:1 ratio of anti-CD3/28 activation beads (Miltenyi) in complete human T cell medium (RPMI1640 from Sigma containing 2 mM glutamine with the addition of 1 mM pyruvate ...
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bioRxiv - Cell Biology 2021Quote: ... gamma tubulin (Sigma T6557). Primary antibodies were detected with secondary antibodies conjugated to IRDye fluorescent probes (LI-COR 926-68021,926-32210) ...
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bioRxiv - Immunology 2023Quote: ... Human interferon-gamma (Sigma) was reconstituted in sterile water and used at 20 ng/ml ...
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bioRxiv - Molecular Biology 2021Quote: Murine CD8+ T cells cultured for 48h with plate-bound anti-CD3/anti-CD28 Abs were stimulated with 2ng/ml PMA (Sigma-Aldrich) and 1μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with primary antibody in blocking solution (1:500 mouse anti-gamma tubulin (Sigma Aldrich, GTU-88), 1:3000 mouse anti-ARL13B (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... The surface of the coverslip was activated by incubation with 22.5 μM of biotin-BSA (Sigma-Aldrich) for 10 min ...
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bioRxiv - Biophysics 2019Quote: ... the free neutravidin on the single molecule surface was concealed with saturated concentrations of biotin (Sigma-Aldrich), prior to addition of the fluorogen ...
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bioRxiv - Neuroscience 2021Quote: ... Remaining surface biotin was cleaved by incubation with glutathione cleavage buffer (50 nM L-Glutathione (Sigma #G6013) 75 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... double immunohistochemistry was performed using primary antibodies for myelin/oligodendrocyte glycoprotein (MOG, Sigma-Aldrich, SAB1406138) and neurofilament (NF ...
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bioRxiv - Immunology 2021Quote: ... The slices were rinsed in TBS-T (5 min each) followed by the addition of secondary antibody (Goat anti-mouse IgG-biotin from Sigma, 1:500) and kept at RT for 2 hours in a humidified chamber ...
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse Cy3 anti-biotin antibody (#C5585 - Sigma) at 1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... Iowa City, IA), dynein heavy chain (P1H4, gift from T. Hays, University of Minnesota, Minneapolis, MN) and anti-HA antibody (Sigma, H 6533 (clone HA-7).
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-gamma tubulin (Sigma) or rabbit anti-acetylated alpha-tubulin (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... gamma-secretase inhibitor DAPT (Sigma) at 10µM concentration and recombinant WNT5B protein (7347 ...
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bioRxiv - Genomics 2023Quote: ... HP1 gamma (Millipore 05-690).
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bioRxiv - Molecular Biology 2024Quote: ... anti-Gamma tubulin (Sigma, T6557), anti-V5 (BioRad ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Microbiology 2021Quote: ... The blocked cells were incubated with an α-HA-biotin primary antibody (Sigma-Aldrich) diluted 1:1,000 into PBS supplemented with 0.25% TX-100 for 30 min at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies used were: mouse anti-gamma tubulin GTU-88 (1:1000 Sigma-Aldrich T6557), rabbit anti-GM130 (1:1000 Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used were anti-gamma H2A.X (phospho S139) (Sigma-Aldrich, #05-636), anti-RPA32/RPA2 (phospho S33 ...
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bioRxiv - Cell Biology 2020Quote: ... Fused cells (myotubes) were visualized using an antibody against Myosin heavy chain isoforms (MyHC) (MF20, Sigma-Aldrich). Primary antibodies were visualized with anti-rabbit or anti-mouse conjugated with Alexa 488 or Cy5 (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... The LRP1 α-chain (515 kDa) and β-chain (85 kDa) were detected using antibodies 8G1 (2μg/mL) (Merck-Millipore) and 5A6 (2μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Cell Biology 2023Quote: Biotin-anti-6xHis antibodies (SIGMA, MA1-21315-BTIN) were flowed in through a streptavidin-coated flow channel ...