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Citations for Millipore Sigma :
1 - 50 of 3741 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... in a T-25 flasks coated with a gelatin solution (Sigma-Aldrich) until confluent ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were revived in a T-25 Corning cell culture flask (CLS430639, Millipore Sigma) with RPMI-1640 supplemented with 0.05 mM 2-mercaptoethanol and 20% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded on T-25 vent-cap flasks pre-coated with fibronectin (Sigma, Missouri, USA) and incubated at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... aliquoted in equal cell numbers to T-25 or T-100 ThermoFisher Tissue Culture Flasks (each flask corresponding to one time point) and treated with Triptolide (Sigma-Aldrich, T3652-1MG) or Trichostatin A (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO3 ...
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bioRxiv - Neuroscience 2024Quote: ... cells from one T-25 flask were dissociated with 2 mL of dissociation reagent containing papain (50U; Sigma-Aldrich; Cat. No. P4762) and Accutase (Thermo-Fischer Scientific ...
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bioRxiv - Immunology 2020Quote: ... the tumour cells were transferred to 25 cm2 culture flasks with RPMI 1640 (Sigma-Aldrich) and 10% foetal calf serum (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: Chondrocytes were seeded in T-175 cell culture flasks (Corning®, Sigma-Aldrich, Oakville, ON, Canada) at a density of 50,000 cells/cm2 (∼9 million cells per flask ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were plated onto 25 cm2 flasks coated with poly-D-lysine (Sigma, MO, USA). The cells were cultured in DMEM-LG containing 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... and 5 µg spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into 5 x 106 HEK293T cells in T-75 flask with lipofectamine 2,000 (Sigma). Six hours post-transfection ...
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bioRxiv - Neuroscience 2022Quote: ... coated flasks T75 flasks in EFH100 medium (Stemline (Sigma), 100ng/ml EGF (Miltenyi Biotec Inc) ...
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bioRxiv - Molecular Biology 2023Quote: ... DGRC #181) were maintained at 25°C in 75 cm2 cell culture-treated flasks in M3 media (Sigma) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: ... the ESC line Hb9::eGFP-CD2 was plated on gelatinized 25 cm2 flasks in DMEM media (Millipore Sigma) containing 15% ES-FBS (Millipore Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded in a T-75 flask previously coated with 10μg/ml poly-D-lysine (Sigma Aldrich, P7280) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... by mixing 5 μl sample with 95 μl of 25 μM Thioflavin-T (Sigma) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with 95 µl of a 25 µM Thiovlavin T (Sigma-Aldrich) in PBS solution for 45 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... And then cells were seeded in 25-cm2 tissue culture flask pre-coated with 0.1% Gelatin (Sigma-Aldrich, USA). When cultures reached 70-80% confluence ...
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bioRxiv - Microbiology 2021Quote: ... mexicana strain M379 (MNYC/BC/62/M379) promastigotes were grown in T-75 flasks at 26 °C in Schneider’s insect medium (Sigma-Aldrich) supplemented with 0.4 g/L NaHCO3 ...
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bioRxiv - Biochemistry 2023Quote: ... Further viral amplifications were performed using adherent Sf9 cells in a T-75 flask containing ESF 921 growth medium plus 5% heat-inactivated FBS (Sigma) at 27 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were passaged as single cell suspension in the range of 1 - 1.5 million cells in a freshly coated T-75 flask with 20 µg/mL laminin (CC095, EMD Millipore). ReNcell was detached from the T-75 flask with Accutase ...
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bioRxiv - Microbiology 2020Quote: ... 1 ml of each aspirate was dispersed in T175 culture flasks containing 25 ml of low glucose Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma, Dorset, UK), supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... Aedes albopictus clone C6/36 cells (ATCC CRL-1660) were grown in 25 cm2 flasks with Eagle’s Minimum Essential Medium (EMEM) (Sigma, M0325, USA) with 10% heat inactivated FBS at 28°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... DKO cells were seeded into 25 cm2 flask in growth medium containing 2 µM 4OH-tamoxifen (Sigma, cat. no. H-6278) for 48 h ...
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bioRxiv - Microbiology 2021Quote: ... These were then diluted 1:100 into baffled flasks containing 25 ml LB-Cm supplemented with either □-(+)-glucose (BDH, #101176K) or □-(+)-arabinose (Sigma-Aldrich, #A3256), both at 0.4% w/v final concentration ...
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bioRxiv - Microbiology 2021Quote: ... in 25 mL Erlenmeyer flasks (cultivation method 1) containing 15 mL of medium and stoppered with a Suba-Seal® (Sigma Aldrich Inc.) septum to maintain oxygen restricted conditions ...
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bioRxiv - Neuroscience 2022Quote: ... Dissociated cells were filtered through a 70-um strainer to collect cell suspension and cultured in 25-mm2 tissue culture flasks on poly-D-lysine-coated (Sigma-Aldrich, Schnelldorf, USA) with 10 ml of medium ...
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bioRxiv - Microbiology 2019Quote: ... Trophozoites were grown in T-75 tissue culture flasks at 30°C in 10 ml ATCC medium 712 (PYG plus additives) (Sigma-Aldrich Corporation, St. Louis, MO) (11) ...
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bioRxiv - Microbiology 2024Quote: Ac trophozoites were grown and maintained in axenic culture at 30°C in T-75 tissue culture flasks in 10 ml ATCC medium 712 (PYG plus additives) with antibiotics (Pen-Strep) (Sigma-Aldrich Corporation, St. Louis, MO) as described previously (21 ...
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Cell Biology 2021Quote: ... on 1% gelatin-coated flasks (Sigma), kept below 70% confluence and used up to passage 6 ...
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bioRxiv - Immunology 2023Quote: ... Pharmacologically-induced clusters were generated by culturing T-cells in 25 ng/ml Phorbol 12,13-dibutyrate (PDBu) (P1269 Sigma) and 500ng/ml ionomycin (I0634 Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Wells were washed four times with PBS-T and 25 μL of prepared Immobilon Western Chemiluminescent HRP Substrate (Millipore #P90719) added to each well immediately prior to the plate being read on a Synergy Neo2 Plate Reader (BioTek Instruments) ...
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bioRxiv - Immunology 2023Quote: ... CD8+ and CD4+ T cells were resuspended in RPMI 1640 with 25 mM Hepes (Sigma-Aldrich, St. Louis, Missouri, USA), supplemented with 10% BCS (Bovine Calf Serum ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... CD8+ T cells were harvested and stained to assess CD107a expression following stimulation with PMA (25 ng/ml, Sigma-Aldrich) and ionomycin (1µM ...
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bioRxiv - Cell Biology 2021Quote: Thioflavin T (ThT, Sigma Aldrich T-3516) was kept as a 10 mM stock solution in distilled water and stored at 4°C in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... sterilized using 0.1 µm filter flask (Millipore), stored up to 1 week in 4°C.
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bioRxiv - Molecular Biology 2021Quote: ... sterilized using 0.1 µm filter flask (Millipore), stored up to 1 week in 4°C.
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bioRxiv - Molecular Biology 2020Quote: ... coated flasks in Claycomb Medium (Sigma-Aldrich) supplemented with 2 mM L-glutamine (Gibco ...
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bioRxiv - Immunology 2020Quote: ... removed from the flask with trypsin (Sigma) and neutralised with trypsin inhibitor (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Flasks were previously siliconized with Sigmacote (Sigma) to prevent mycelium adhesion and IC formation on the glassware during culture ...
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bioRxiv - Biophysics 2019Quote: ... were plated under identical conditions in T-25 culture flaks and cultured in DMEM medium supplement with 10% FBS (Sigma F7524) and 1% Pen Strep (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The contribution of L-type voltage gated Ca2+ channel activation to t-LTP was evaluated with bath applied Nifedipine (25 μM, Sigma, Germany). To interfere with group I metabotropic glutamate receptor (mGluR ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in culture medium (1.5 brains in 10ml per flask) and grown in vented T75 flasks coated with poly-D-lysine (PDL; Sigma-Aldrich; 5µg/ml in water ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... LSECs were detached from flasks using Trypsin (Sigma) and collected accordingly ...
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bioRxiv - Immunology 2022Quote: ... grown in CELLLine CL-1000 flasks (Sigma-Aldrich) using protein A-sepharose 4B beads (pro-A beads ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were mechanically dissociated and plated on T25 flask (one brain per flask) coated with poly-D-lysine (0.05 mg/mL; Sigma, catalog #P7886) in Dulbecco’s Modified Eagle Medium (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of thawed filtered lentivirus was used to transduce approximately 2.25×106 HFF-T or 3×106 RPE cells in 10 cm plates with 8 µg/mL polybrene (Millipore-Sigma: TR-1003-G). Cells were allowed to reach confluence before selection with 1 µg/mL puromycin for 3 days ...
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bioRxiv - Biochemistry 2019Quote: ... in 125 ml shaking flasks (Sigma Aldrich, CLS431143-50EA) and cultivated in a fed batch cultivation for 13 days ...
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bioRxiv - Microbiology 2020Quote: ... 8% CO2 in culture filter cap conical flasks (Sigma) on a rotation platform (125 rotations/min ...
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bioRxiv - Neuroscience 2022Quote: ... T25 flasks in DMEM high glucose medium (Sigma D5671) containing 15% FBS (Sigma S0615) ...