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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... FCS was replaced with 5% human serum (Sigma-Aldrich, H6914). Hypersalinity (high NaCl ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % FCS (Sigma) in DPBS for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Immunology 2020Quote: ... 5% FCS (Sigma-Aldrich), 20 μg/ml gentamycin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Immunology 2019Quote: ... 5% (v/v) FCS (Sigma). The following day ...
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bioRxiv - Biophysics 2023Quote: ... with 5% FCS (Sigma-Aldrich) and 0.5% Penicilin/Streptomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5% FCS (Sigma), 1% Pen-Strep (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... a Goat Anti-human Fc HRP (Sigma) was used as secondary antibody with 1:6000 dilutions ...
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bioRxiv - Microbiology 2022Quote: ... 5-fluorouracil (5-FC, Sigma-Aldrich, cat#F6627) was prepared in drinking water at a final concentration of 2.0 mg/ml ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Biochemistry 2019Quote: Cell lysate from HEK293 transfected with FLAG-Podocin (WT, R45A, R50 and R54A) was immobilized on anti-FLAG beads (Sigma-Aldrich). The beads were washed with IP buffer (1%–2% Triton-X ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 5% FCS (Sigma, #F4135), 1% penicillin (10,000 Units/ml ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 1:5 000 FLAG (Sigma, F1804), 1:10 000 AR (Santa Cruz ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg FLAG antibodies (Sigma F1804) were used for each experiment and 25 μl slurry Protein A/G UltraLink Resins (ThermoFisher 53133 ...
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bioRxiv - Microbiology 2021Quote: ... Flag (1:5, 000; Sigma, F1804) were used for protein detection ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated goat anti-human IgG Fc (Sigma-Aldrich) was used for detection ...
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bioRxiv - Microbiology 2020Quote: ... anti-human Fc-conjugated to alkaline phosphatase (A9544, Sigma) was added (50 µl/well ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS and human serum AB (Sigma) for 2 days at 107 cells/ml in 24-well plate following “RESTORE” protocol (67 ...
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bioRxiv - Immunology 2020Quote: ... goat anti-human IgG (Fc) peroxidase conjugate (Sigma A0170) for 45 min ...
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bioRxiv - Immunology 2021Quote: ... using an anti-human Fc mAb (Sigma-Aldrich, I6260) as capture ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293-ACE2 cells [HEK293 cells (ATCC CRL-1573) stably expressing human ACE2]22 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Neuroscience 2021Quote: ... polyclonal antiserum against Siglec-10 (1:50, HPA027093, Sigma); Monoclonal Antibody Siglec-F (CD170 ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) prepared at 1:3000 in PBST was then added and plates were incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... plates were coated with anti–human Fc (Sigma, Catalog # FAB3700259) and goat anti–human HRP–conjugated IgG (H+L ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 5% heat inactivated FCS (Sigma Aldrich cat. # 12133C) and Penicillin-Streptomycin (100 units/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... including 5-fluorocytosine (FC; Cat: 543020; Sigma Aldrich), 5-fluorouracil (FU ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg anti-FLAG antibody (Sigma, F3165) per gram of tissue were added to the supernatant in a new tube and incubated at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl of FLAG beads (Sigma-Aldrich) were added for FLAG IPs ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μg FLAG M2 antibody (F1804, Sigma) was pre-bound to 40 μL protein G Dynabeads (10003D ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-Flag (Sigma-Aldrich F3165, 5 μg), anti-HA (Santa Cruz Biotechnology sc-7392 ...