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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Microbiology 2021Quote: ... for SARS CoV-2 spike detection mouse anti-spike (Cat# ZMS1076, Sigma Aldrich) respectively ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S1 domain (ACRO biosystems – S1N-C52H3) or BSA (Sigma – A7906) overnight at 4°C in 50 μl/well ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike expressing expi293F cells were stained with PKH26 (Sigma-Aldrich, St-Louis, MO). Cells were then washed with and resuspended in R10 media ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Molecular Biology 2022Quote: ... Conjugates to horseradish peroxidase of anti-sheep IgG from donkey (Sigma-Aldrich, A3415), or anti-mouse IgG from goat (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ADCP was performed using CHO cells stably expressing full-length wild-type SARS-CoV-2 S glycoprotein (target cells) fluorescently labelled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich; Cat. Nr.: MINI67). Target cells were incubated with titrated concentrations of mAbs for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Secondary antibodies were sheep horseradish peroxidase (HRP)–coupled anti-mouse IgG (Sigma-Aldrich, GENA931) and donkey HRP–coupled anti-rabbit IgG (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase (HRP)-conjugated mouse anti-FLAG tag antibody (A8592, Sigma-Aldrich) was used to detect VH binding ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... horseradish peroxidase (HRP)-conjugated anti-human IgG Fc specific antibody (Sigma, USA) was added at the dilution of 1:2,000 and incubated at 37 °C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... or horseradish peroxidase-conjugated goat anti-human IgG Fc antibody (Sigma, A0170) diluted 1:20,000 in the blocking buffer for an hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
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bioRxiv - Immunology 2022Quote: SARS-COV-2 viral RNA was extracted using TRI Reagent (Sigma, USA). Reverse transcription was performed using HiScript II Q RT SuperMix for qPCR (Vazyme ...
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bioRxiv - Immunology 2020Quote: ... rabbit anti-SARS-CoV S polyclonal sera (BEI Resources) and mouse anti-2xStrep-tag antibody (Sigma-Aldrich). Anti-mouse IgG AF555 ...
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bioRxiv - Neuroscience 2020Quote: ... horseradish peroxidase-conjugated (Millipore) or biotinylated (Jackson Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... horseradish peroxidase substrate (Millipore) was applied ...
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bioRxiv - Cell Biology 2022Quote: ... and SARS-CoV-2 was inactivated with 1% Triton-X (Sigma-Aldrich, Burlington, MA) in PBS for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... anti-mouse-IgG horseradish peroxidase or goat anti-rabbit-IgG horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10,000 for one hour (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... and horseradish peroxidase (HRP; Sigma) and stimulated with 100nM PMA ...
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bioRxiv - Cancer Biology 2021Quote: ... 1.4μM horseradish peroxidase (Sigma, #P6782), 133μM DTT ...
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bioRxiv - Developmental Biology 2021Quote: ... Horseradish peroxidase-conjugated antibodies (Millipore) were used as secondary antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... horseradish peroxidase (P6782; Sigma-Aldrich), and 1 μM flg22 (RP19986 ...
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bioRxiv - Microbiology 2021Quote: MERS-CoV and SARS-CoV-2 strains were obtained from different collaborators and passaged once in VeroE6 cells in DMEM (Sigma Aldrich) supplemented with 2% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2-infected cells were lysed in radio-immunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with 1% sodium dodecyl sulfate ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Peroxidase conjugated sheep anti mouse IgG Ab (Sigma) at a 1:1000 dilution was used as secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase coupled secondary antibodies (Sigma) and Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... 2U/ml horseradish peroxidase (Sigma P8125), 100μM Amplex Red (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase(HRP) conjugated IgG (Sigma) was used to develop the blot ...
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bioRxiv - Biochemistry 2022Quote: ... Horseradish peroxidase (HRP; Sigma P8250-5ku) was prepared at a concentration of 10 U/mL (45.5 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Specific horseradish peroxidase conjugates (Sigma, USA) were employed and OPD (Sigma ...
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bioRxiv - Immunology 2022Quote: ... and horseradish peroxidase type II (Sigma), and incubated the plate at 37°C in 5% CO2 for one hour ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (Sigma Aldrich, United States) reduces hydrogen peroxide to H2O using Amplex Red (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... and 40μM horseradish peroxidase (Sigma Aldrich). Chemiluminescence measurements were taken every minute over 30 min.
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bioRxiv - Plant Biology 2022Quote: ... or Immobilon western horseradish peroxidase (Millipore). For IP ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of Horseradish Peroxidase (Sigma) from 20 mg/mL stock solution in ddH2O ...
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bioRxiv - Plant Biology 2023Quote: ... 20 μg/mL horseradish peroxidase (Sigma), and 100-250 nM of the indicated PAMP/DAMP ...