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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... recombinant human type I or type III collagen (Millipore Sigma) were immobilized on a CM5 sensor chip (Cytiva ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Microbiology 2022Quote: ... Type V collagen from human placenta (Sigma Type IX ...
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bioRxiv - Immunology 2023Quote: ... or human type I collagen (Sigma-Aldrich) overnight ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary anti-Collagen Type II monoclonal antibody (clone 6B3, Millipore), anti-NFATc1 (clone 7A6 ...
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bioRxiv - Microbiology 2020Quote: ... collagen (rat tail type 1, Sigma)-coated coverslips were coated in 35% (v/v ...
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bioRxiv - Immunology 2020Quote: ... and collagen (bovine type 1, Sigma) coated plates in Keratinocyte Growth Medium 2 (KGM-2 ...
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bioRxiv - Genomics 2020Quote: ... human collagen type III (88 ng per well; Sigma-Aldrich) or a combination of human collagen type I and type III (88 ng of each collagen type per well) ...
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bioRxiv - Bioengineering 2023Quote: ... we prepared Human Placenta Collagen Type IV (Sigma, catalog #C7521) according to protocols established by University of North Carolina ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Type V collagen from human placenta (Sigma Type IX, C3657, Sigma-Aldrich, St Louis, MO) was pre-solubilized in aqueous acid and diluted in carbonate coating buffer ...
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bioRxiv - Microbiology 2019Quote: ... filter’s membranes were coated with type IV human placenta collagen (Sigma) for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... and 25 µg/ml type IV collagen from human placenta (Sigma) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 100μg/mL Matrigel (Corning ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL human plasma fibronectin (Corning ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Freund’s complete adjuvant and Collagen Type II were obtained from Sigma-Aldrich.
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bioRxiv - Bioengineering 2021Quote: ... or type I collagen microchannels coated overnight with 50 μg mL−1 human placental collagen IV (Cat. no. C5533, Sigma-Aldrich) and 25 μg mL−1 fibronectin from human plasma (Cat ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Collagen type I (Sigma-Aldrich SAB4200678; 1:300), H3K27me3 (Cell Signalling 9733 ...
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bioRxiv - Physiology 2022Quote: ... gradient and seeded on Collagen type 1 (Sigma) coated plates with HBM basal medium+ HCM single Quotes (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... and 330µg/ml Collagen type 1 (Sigma-Aldrich) coated glass coverslips ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Immunology 2020Quote: 3D Collagen type I was prepared using 8 parts rat collagen type I (Sigma) and 1 part 10x DMEM and was neutralized to pH 7.0-7.5 by adding 0.5 parts 0.5N NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were coated with Type-1 collagen (Sigma # C9791), air-dried for 20 minutes in the laminar hood and exposed to UV light for cross-linking of collagen for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... collagen type IV (Sigma-Aldrich), and fibronectin (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: ... collagen type IV (Sigma C5533) and fibronectin (Sigma 1141 ...
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bioRxiv - Biochemistry 2022Quote: ... Collagen type IV (Sigma C5533) was coated on 96 well plates and left for 6 hours or more before using the plates whenever seeding PC-12 cells.
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Pathology 2021Quote: Cell culture inserts were coated with human placental type IV collagen (Sigma-Aldrich, C7521). CRC expanded cells were seeded at 2.5 × 105 cells on 12-mm Snapwells (Corning Costar ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes had been coated with 0.125 mg/mL type II collagen (C9301, Sigma-Aldrich) in 0.1 M acetic acid ...
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bioRxiv - Molecular Biology 2019Quote: ... Type I collagen (1:200, AB765P, Merck Millipore, Burlington, Massachusetts), α-Actin (1A4 ...
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bioRxiv - Cell Biology 2023Quote: ... on 200 μg/mL rat collagen type 1-coated (Sigma) plates ...
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bioRxiv - Bioengineering 2021Quote: ... and collagen type I (Millipore, E2729) were used for bioink formulation ...
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bioRxiv - Cancer Biology 2022Quote: ... and collagen type I (Sigma, C8919). On the following day ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated with mouse primary anti-Collagen Type II (DSHB II-II6B3) or mouse anti-dopamine 4 receptor (MABN125, Millipore) primary for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Cell Biology 2020Quote: ... pre-coated with human type IV collagen (Cat# C7521, Sigma Aldrich, St. Louis, MO, USA) with 1 ml of BLEAM in the basolateral chamber (ALI day −2) ...
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bioRxiv - Biochemistry 2024Quote: ... we monitored unfolding of kinetics of native collagens type II (bovine tracheal cartilage; Sigma-Aldrich C1188), type IV (human placenta ...
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bioRxiv - Immunology 2020Quote: ... and type II neuraminidase (1/2000) (Sigma) for 1 h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... type II (1:300; B7151; Sigma-Aldrich) and type X (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Inserts was pre-coated with 1 % collagen type I solution (SIGMA) in PBS for 45 min ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-COLLAGEN type IV (cat. # AB756P, Merck Millipore, 1:500), mouse anti-Neurofilament (cat ...
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bioRxiv - Microbiology 2022Quote: ... were coated with 1 μg of collagen type IV (Sigma-Aldrich) or fibronectin (Calbiochem ...