Labshake search
Citations for Millipore Sigma :
1 - 50 of 8880 citations for Recombinant E. coli MUG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
-
bioRxiv - Molecular Biology 2021Quote: Recombinant proteins were expressed in Escherichia coli RosettaTM (DE3) (Millipore) by induction overnight with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
-
bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
-
bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 4-MUG (Sigma) working solutions were prepared as well as internal standards and quenching solutions (25/50 ng/mL Terfenadine (Sigma)/Tolbutamide ...
-
bioRxiv - Biochemistry 2023Quote: ... LPS (E. coli O111:B4, Sigma) was dissolved in PBS to make a stock solution then sonicated in a water bath for 10 min prior to addition to cell suspensions ...
-
bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
-
bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
-
bioRxiv - Cancer Biology 2021Quote: LPS derived from Escherichia coli (E. coli) was from Sigma-Aldrich Canada (Oakville ...
-
bioRxiv - Microbiology 2023Quote: ... coli strain BL21 (DE3) (E. coli BL21, EMD Millipore, Cat#69450). F ...
-
bioRxiv - Plant Biology 2024Quote: ... 1 mM 4-MUG (, M9130-Sigma) and incubated at 37 °C for 6 h ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
-
bioRxiv - Cancer Biology 2019Quote: E coli Rosetta (DE3) pLysS cells (Novagen) transformed with pOPIN-B plasmids encoding wtGATA3 and neoGATA3 were grown in autoinduction media supplemented with 50 µg/ml kanamycin and 34 µg/ml chloramphenicol for 8 h at 37 °C followed by 21 h at 20 °C ...
-
bioRxiv - Biochemistry 2023Quote: Escherichia coli (E. coli) Rosetta2(DE3)pLysS cells (Novagen, cat. no. 71403) were transformed with pET28a-MDA5ΔL2-His6 and grown in 2xTY medium to OD600 0.4-0.6 at 37°C ...
-
bioRxiv - Plant Biology 2019Quote: ... The MUG assay buffer is same as that of the extraction buffer supplemented with 1 mM MUG (Sigma). The reaction was incubated at 37°C water bath for 20 min ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Phytohemagglutinin at 10 /mug/ml (Sigma-Aldrich) was added to activate cells.
-
bioRxiv - Biochemistry 2022Quote: ... 50ng/mL LPS (E. coli O111:B4, Sigma) and 10ng/mL IFN-γ (R&D Systems ...
-
bioRxiv - Microbiology 2019Quote: ... ampicillin (Sigma; 100 μg/mL for E. coli), gentamicin (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: LPS (Sigma Aldrich, E. coli, serotype 0111:B4), was prepared by dissolving 0.3 mg of LPS in 1ml of PBS (Phosphate buffer saline) ...
-
bioRxiv - Bioengineering 2022Quote: ... and LPS (lipopolysaccharides from E. coli; Sigma L8274); HEK-293Ta showed the strongest response to TNFα and were thus selected as responder cells in the co-culture assay (Fig ...
-
bioRxiv - Microbiology 2022Quote: ... or tRNA (from E. coli MRE 600, Sigma) was incubated with PT mutants ...
-
bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
-
bioRxiv - Biochemistry 2023Quote: ... and expressed in BL21 Rosetta 2 (DE3) pLysS Escherichia coli (E. coli) cells (Novagen). All plasmids were verified by Sanger sequencing (OSU Shared Resources) ...
-
bioRxiv - Immunology 2020Quote: Alexa fluor 488 labelled bacteria (E. coli, Sigma-Aldrich) and yeast (S ...
-
bioRxiv - Immunology 2021Quote: ... LPS (E. coli serotype O55:B5, Sigma, 10ng/ml), CpG ODN 1826 (TIB MOLBIOL ...
-
bioRxiv - Neuroscience 2023Quote: LPS (E. coli, O111:B4 serotype, Sigma Aldrich, UK) was dissolved at 0.5 mg/ml in sterile 0.9% saline ...
-
bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
-
bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
-
bioRxiv - Microbiology 2020Quote: Overexpression of was performed by using Escherichia coli (E. coli) Rosetta (DE3) pLysS cells (Novagen) harboring the E ...
-
bioRxiv - Plant Biology 2021Quote: ... a 4-methylumbelliferyl b-D-glucuronide (MUG) (Sigma-Aldrich) assay was carried out as previously described (He et al. ...
-
Neuraminidase inhibitors rewire neutrophil function in vivo in murine sepsis and ex vivo in COVID-19bioRxiv - Immunology 2022Quote: ... LPS (1 µg/mL, E. coli 0127:b8, Sigma-Aldrich), LPS plus Oseltamivir or LPS plus Zanamivir ...
-
bioRxiv - Microbiology 2022Quote: ... 0.2 U Pyrophosphatase (E. coli, MFCD00131379 from Sigma-Aldrich®), 0.127 mg.ml−1 MtATPS ...
-
bioRxiv - Neuroscience 2022Quote: ... or lipopolysaccharide (LPS, Sigma-Aldrich, E. coli, O111:B4, L4391) dissolved in PBS at a dose of 5 mg/kg (cohort 1 ...
-
bioRxiv - Bioengineering 2023Quote: mTmG mice were treated with 100 µg Escherichia coli (E. coli, serotype O55:B5, Sigma-Aldrich) lipopolysaccharide (LPS ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
-
bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
-
bioRxiv - Biochemistry 2021Quote: ... the whole operon of FhuCDB was cloned from Escherichia coli (E. coli) genome into a pET15b (Novagen) vector with a His-tag and a thrombin digestion site on the N-termini of FhuC ...
-
bioRxiv - Molecular Biology 2022Quote: ... The MUG (4-Methylumbelliferyl β-D-glucuronide) (Sigma-Aldrich, M9130) and luciferase assay system (Promega ...
-
bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
-
bioRxiv - Immunology 2019Quote: ... or LPS Ultrapure (standard LPS, E. coli 0111:B4; Sigma-Aldrich); or with the negative control for cell stimulation (the medium) ...
-
bioRxiv - Immunology 2020Quote: ... Purified E coli flagellin and LPS (Sigma, St Louis, Missouri, USA) were used as positive controls for HEK-Blue-mTLR5 and HEK-Blue-mTLR4 cells ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... we diluted LPS (from serotype 055:B5 E. coli; Sigma-Aldrich) to 0.5mg/mL in a sterile physiological saline (Ringer’s solution ...
-
bioRxiv - Immunology 2020Quote: ... or LPS (0.2μg ml−1, E. coli O55:B5 Sigma-Aldrich) in the presence of M-CSF (10ng ml−1 ...
-
bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
-
bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
-
bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were incubated with 50ng/mL LPS (E. coli O111:B4, Sigma) and 30ng/mL IFN-γ (R&D Systems).
-
bioRxiv - Immunology 2020Quote: We used LPS (from E. coli serotype O55:B5, Sigma, Rehovot, Israel) or human TNFα (Peprotech ...
-
bioRxiv - Immunology 2022Quote: ... ii) 50 ng/ml LPS (E. coli 0111:B4, Sigma-Aldrich L288) for 2 days then with 20 ng/ml INF-γ (ImmunoTools ...