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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Rabbit IgG Anti SARS CoV 2 Spike S1 Antibody CR3022 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Microbiology 2021Quote: ... for SARS CoV-2 spike detection mouse anti-spike (Cat# ZMS1076, Sigma Aldrich) respectively ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S1 domain (ACRO biosystems – S1N-C52H3) or BSA (Sigma – A7906) overnight at 4°C in 50 μl/well ...
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bioRxiv - Microbiology 2023Quote: ... of anti-spike murine antibodies (including cross-reactive mAbs against SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2024Quote: ... and -71 60 of anti-spike murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike expressing expi293F cells were stained with PKH26 (Sigma-Aldrich, St-Louis, MO). Cells were then washed with and resuspended in R10 media ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Immunology 2020Quote: ... rabbit anti-SARS-CoV S polyclonal sera (BEI Resources) and mouse anti-2xStrep-tag antibody (Sigma-Aldrich). Anti-mouse IgG AF555 ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL, anti-SARS-CoV-2 N mAb, clone 1G10C4 mAb)42 and anti-β-actin antibody (Sigma-Aldrich, cat ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Microbiology 2020Quote: ... rabbit polycolonal anti-SARS-CoV-2 S2 antibodies (1:1000) (Sinobiological Inc, Beijing, China) and anti-FLAG M2 antibody (1:1000) (Sigma, St. Louis, MO, USA), respectively.
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with CR3022 anti-S antibody (1 ug/mL)128 overnight followed by anti-human IgG-HRP-conjugated goat secondary antibody (Sigma, 1:500) for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Genomics 2022Quote: ... Infectious foci were stained with a human anti-SARS-CoV-2 Spike antibody (H2-162, Hugo Mouquet’s laboratory, Institut Pasteur) and the corresponding HRP-conjugated secondary antibody (Sigma-Aldrich). Foci were visualized by 3,3’-diaminobenzidine staining solution (DAB ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Plant Biology 2019Quote: ... Rabbit anti-rat IgG antibody (Sigma) diluted 1:30000 in 5% milk powder (w/v ...
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bioRxiv - Immunology 2022Quote: SARS-COV-2 viral RNA was extracted using TRI Reagent (Sigma, USA). Reverse transcription was performed using HiScript II Q RT SuperMix for qPCR (Vazyme ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti Rabbit IgG peroxidase antibody (SIGMA, A0545) and Anti ArHm IgG peroxidase antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Goat anti-rabbit IgG-peroxidase antibody (Sigma) was used as a secondary antibody for mCherry ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 hours with secondary antibody (Goat anti-mouse IgG-HRP or Goat anti-rabbit IgG-HRP, Sigma) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and SARS-CoV-2 was inactivated with 1% Triton-X (Sigma-Aldrich, Burlington, MA) in PBS for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... a pooled rhesus serum collected day 14 after infection with SARS CoV-2 was used as standard after depletion of IgG using GE Healthcare Protein G Sepharose (Sigma, St. Louis, MO) as described (52) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Rabbit IgG Alkaline Phosphatase antibody (Sigma Aldrich), anti-mouse IgG Alkaline Phosphatase antibody (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... a secondary rabbit anti-bovine IgG antibody (Sigma) diluted 1:20,000 was incubated for one hour and washed three times with PBST before adding colourimetric phosphatase substrate and acquiring absorbance readings as described above.
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bioRxiv - Cell Biology 2021Quote: ... anti-Rabbit IgG Alkaline Phosphatase antibody (Sigma Aldrich), anti-mouse IgG Alkaline Phosphatase antibody (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: ... primary antibodies and anti-rabbit IgG secondary antibody (Sigma, NA934VS) according to standard techniques ...
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bioRxiv - Microbiology 2021Quote: MERS-CoV and SARS-CoV-2 strains were obtained from different collaborators and passaged once in VeroE6 cells in DMEM (Sigma Aldrich) supplemented with 2% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2-infected cells were lysed in radio-immunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with 1% sodium dodecyl sulfate ...