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Citations for Millipore Sigma :
1 - 50 of 5406 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: ... SybrGreen Quantitative RT-PCR kit (Sigma Aldrich, Germany) was used with Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Gene expression was measured by quantitative RT-PCR and normalized to β-actin8,48 The SYBR Green quantitative RT-PCR kit (Sigma) was used with the following primers ...
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bioRxiv - Microbiology 2021Quote: ... For gene expression analysis one-step qRT-PCR (SYBR Green Quantitative RT-PCR kit, Sigma) was carried out ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
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bioRxiv - Immunology 2023Quote: ... and RT-PCR amplification data (Sigma). Samples were pooled and normalized to 10nM ...
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bioRxiv - Physiology 2020Quote: ... The mouse RT-PCR primers (Sigma-Aldrich) used are shown in Supplementary Tab ...
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bioRxiv - Microbiology 2020Quote: We performed reverse transcription using the First strand cDNA synthesis kit for RT-PCR (AMV) (Sigma-Aldrich) and the protocol described by the supplier ...
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bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR was performed using Kapa SYBR Fast (Sigma). SREBP genes primer sequences are listed in Supplementary Table S3 ...
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bioRxiv - Molecular Biology 2020Quote: RT-PCR: 106 cells were lysed in TRI reagent (Sigma) and RNA extracted using Direct-zol columns with a 30 minute on-column DNase step at room temperature (Zymo Research) ...
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bioRxiv - Bioengineering 2020Quote: ... The nucleotide sequences for RT-PCR and real-time PCR primers (synthesized by Sigma-Aldrich) are shown in Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... Samples for quantitative PCR and quantitative RT-PCR were homogenized in TRiZOL reagent (Sigma Aldrich) and further processed for nucleic acid extractions using manufacturer’s protocols.
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-QPCR was performed in triplicate for each samples using the KAPA SYBR PCR kit (Sigma Aldrich KK4600) with a LightCycler 480 apparatus (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR products were separated by gel electrophoresis in 1% agarose (Sigma). Gene-Ruler 100 bp DNA ladder (ThermoScientific ...
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bioRxiv - Cell Biology 2021Quote: ... qRT-PCR was performed with Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07; Sigma). Inventoried TaqMan probes for qRT-PCR from Applied Biosystems were used for human TULP3 and GAPDH ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR was carried out using SYBR Green (Jumpstart Taq ReadyMix: Sigma-Aldrich) in 10 μl volumes for 3 biological and 3 technical replicates with 1:10 diluted cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using FastStart Universal SYBR Green Master (Rox) (Sigma, 4913850001) on a Bio-Rad CFX Connect Real-Time PCR detection system ...
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bioRxiv - Cell Biology 2020Quote: ... the TRAPEZE® RT Telomerase Detection Kit (Chemicon; EMD Millipore) was utilized ...
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bioRxiv - Cancer Biology 2021Quote: ... FastStart PCR Kit (Sigma-Aldrich) was used for PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... KAPA SYBR PCR kit (Sigma) was used for QPCR (Light Cycler 480 ...
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bioRxiv - Microbiology 2020Quote: ... The 3’UTR was determined by RT-PCR with an oligo-dT anchor primer (Sigma) as described58 with primers found in Table 4 ...
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bioRxiv - Microbiology 2020Quote: ... RT and PCR primers for the determination of the 3’UTR were obtained from Sigma. The remaining primers were purchased from TAG Copenhagen.
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bioRxiv - Cancer Biology 2021Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) (13–16) ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR analysis was performed using the KAPA SYBR Fast PCR master mix (Sigma Aldrich, #KK4605) or SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR on both gDNA and cDNA was performed using the SYBR® Green JumpStart (Sigma) in a 7500 Fast Real Time PCR cycler from Applied Biosystems.
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bioRxiv - Microbiology 2020Quote: ... presence of enzyme inhibitors in RNA extracts was assessed by reverse transcription PCR (eAMV RT, Sigma-Aldrich) using universal primers for 18S RNA ...
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bioRxiv - Microbiology 2020Quote: For real-time quantitative reverse transcription PCR (RT-qPCR) total cellular RNA was extracted with Trizol (Sigma) or QiAmp (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ...
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bioRxiv - Microbiology 2022Quote: Quantitative real-time PCR (rt-qPCR) was carried out using the KAPA SYBR® FAST Mix (Sigma) on a LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Genetics 2021Quote: ... All PCR products were purified with GenElute PCR Clean-Up Kit (Sigma) and sent for Sanger sequencing (Microsynth ...
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bioRxiv - Neuroscience 2020Quote: ... The KAPA Taq PCR kit (Sigma, BK1000) was used in conjunction with three primer sequences (wild type ...
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bioRxiv - Plant Biology 2023Quote: ... the KAPA3G Plant PCR Kit (Sigma-Aldrich) was used to amplify an 824 bp fragment spanning the gene target site using an initial touchdown for 6 cycles with annealing at 70°C decreasing by 1.6°C per cycle followed by 34 cycles annealing at 62.1°C ...
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bioRxiv - Biochemistry 2023Quote: Mycoplasma PCR Detection Kit (LookOut, Sigma-Aldrich) Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The SYBR Green quantitative PCR kit (Sigma) was used with the following primers ...
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bioRxiv - Microbiology 2019Quote: ... RT-PCR was performed on 100 ng of cDNA in triplicate using SYBR Green Jumpstart Taq readymix (Sigma). Primers RMC379/380 (16S internal) ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The cDNA samples generated with RT PCR were then used for performing qPCR using SYBR Green I (Sigma) and Platinum Taq polymerase (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich), after restriction digestion of the PCR mixture with DpnI (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... PCR purification was performed using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Gibson assembly was done using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was purified by GenElute PCR Clean-Up Kit (Sigma-Aldrich) and cloned into pCR-Blunt II-TOPO vector by Zero Blunt TOPO PCR Cloning Kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... PCR purification was conducted using GenElute™ PCR Clean-Up Kit (Sigma Aldrich) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... RT-PCR was performed in a Biorad CFX384 using KiCqStart SYBR Green Ready Mix (Millipore-Sigma, St. Louis, MO). RNA expression was normalized to the housekeeping gene GADPH and quantified using the ΔΔCt method ...
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bioRxiv - Neuroscience 2019Quote: ... RT-PCR was performed in a Biorad CFX384 using KiCqStart SYBR Green Ready Mix (Millipore-Sigma, St. Louis, MO). RNA expression was normalized to the housekeeping gene GADPH and quantified using the ΔΔCt method ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020). The expression vector pET302/NT-His was digested with EcoR1 restriction enzyme (NEB R0101S ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Samples were stored overnight at −20°C prior to library construction.
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bioRxiv - Genomics 2021Quote: PCR barcoding was performed using Kapa 2G Robust PCR kit (Sigma Catalogue No. KK5005) as follows ...
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bioRxiv - Genetics 2022Quote: ... all PCR products were amplified using Roche Expand High Fidelity PCR kit (Sigma 11732641001), which contains a 3’-adenine overhang-depositing Taq ...
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bioRxiv - Cell Biology 2020Quote: ... After purification with PCR Cleanup kit (Sigma-Aldrich), nucleic acids were eluted in 100 μL of elution buffer (5 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2022Quote: The KAPA Taq PCR Kit (Sigma-Aldrich KR0352_S) was used to amplify the reads from a cDNA mixture of uninfected samples and 1 ...
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bioRxiv - Cell Biology 2023Quote: ... The LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) was used to assure that all cell lines were mycoplasma-free ...