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Citations for Millipore Sigma :
1 - 50 of 6345 citations for Pyruvate Dehydrogenase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: PDH activity was measured using Pyruvate Dehydrogenase Activity Assay Kit (MAK183-1KT, Sigma) as recommended by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ... The conversion of pyruvate to lactate by lactate dehydrogenase was assessed with a pyruvate assay kit (Sigma, MAK332), and the color change of X-gal was observed in the channels and buffers for the β-galactosidase assay.
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bioRxiv - Biophysics 2021Quote: ... 9-15 U/mL pyruvate dehydrogenase (Sigma), 13.5-21 U/mL lactate dehydrogenase (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... and lactate dehydrogenase/pyruvate kinase mix (Sigma) were added to unwinding reactions to a final concentration of 2 mM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lactate dehydrogenase (LDH) Activity Assay Kit (Sigma-Aldrich, MAK066), Adenosine Triphosphate (ATP ...
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bioRxiv - Biochemistry 2022Quote: The lactate dehydrogenase colorimetric assay (Abs450) kit (Sigma, MAK066) measures LDH activity in a biological sample based on reduction of NAD to NADH ...
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bioRxiv - Microbiology 2019Quote: Cellular pyruvate level was quantified using Pyruvate Assay Kit (Sigma) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of 0.57 U/mg pyruvate dehydrogenase (Sigma) at 12.5 mg/mL ...
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bioRxiv - Microbiology 2019Quote: ... 20 units/ml pyruvate kinase and lactate dehydrogenase (Sigma–Aldrich), and 0.5 mM GTP ...
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bioRxiv - Genetics 2020Quote: ... 2 units of pyruvate kinase/lactate dehydrogenase mixture (Millipore Sigma), and varying concentrations of AK2 (0-40 nM) ...
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bioRxiv - Biochemistry 2023Quote: ... The protocol outlined in the Malate Dehydrogenase Assay Kit by Sigma was followed to measure the renaturation of denatured hMDH using each chaperonin preparation.
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bioRxiv - Evolutionary Biology 2019Quote: ... saturating amounts of pyruvate kinase and lactate dehydrogenase (Sigma-Aldrich, P0294), and varying amounts of ATP (0.01 to 8 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... and 1/100 (v/v) Pyruvate Kinase/Lactic Dehydrogenase mix (Sigma). 100 µl of 4 mM ATP in assay buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... the amount of pyruvate was quantified using a Pyruvate Assay Kit (Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: AMPK3 and 4 activity was determined using a coupled enzyme assay with pyruvate kinase and lactate dehydrogenase (LDH, Sigma, P0294), measuring the production of NAD+ that results in absorption-decrease at 340 nm ...
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bioRxiv - Biophysics 2020Quote: ... 0.04 U/μl pyruvate kinase/ 0.05 U/μl lactic dehydrogenase (Sigma-Aldrich) and 5 mM ATP ...
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bioRxiv - Biochemistry 2019Quote: ... 100 units/mL lactate dehydrogenase and 80 units/mL pyruvate kinase (Sigma) in buffer composed of 50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.04 U/μl pyruvate kinase/0.05 U/μl lactic dehydrogenase (Sigma-Aldrich) and 5 mM ATP ...
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bioRxiv - Bioengineering 2023Quote: ... The conversion of pyruvate to lactate was quantified using a pyruvate assay kit (Sigma) to evaluate the effectiveness of these strategies ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mM NADH and 25 units/mL lactate dehydrogenase/pyruvate kinase (Sigma-Aldrich) to monitor the NADH dependent fluorescence signal in non-binding ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 mM sodium dichloroacetate (an inhibitor of pyruvate dehydrogenase kinase, Sigma-Aldrich) was used ...
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bioRxiv - Biochemistry 2022Quote: ... 10 u/ml pyruvate kinase/lactate dehydrogenase (from rabbit muscle, Sigma Aldrich, #P0294) and 5 mM MgCl2 in SEC buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Excess pyruvate kinase (PK) / lactate dehydrogenase (LDH) enzymes from rabbit muscle (Sigma P0294), ADP ...
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bioRxiv - Biochemistry 2019Quote: ... 20 units of enzymes (lactate dehydrogenase (LDH) and pyruvate kinase (PK)) (Sigma-Aldrich), 4 mM ATP ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mM NADH and 25 units/mL lactate dehydrogenase/pyruvate kinase (Sigma-Aldrich) to monitor the NADH dependent fluorescence signal in non-binding ...
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bioRxiv - Biochemistry 2020Quote: ... 5.6 U/ml pyruvate kinase/ 7.8 U/ml lactate dehydrogenase (Sigma, cat: P0294), TFIIH (1.2 nM yeast variants or 6 nM human variants) ...
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bioRxiv - Biochemistry 2020Quote: ... and pyruvate kinase/lactic dehydrogenase enzyme mix (diluted final 1:45; Sigma, P0294). Where indicated 3 mM CaCl2 was present ...
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bioRxiv - Biophysics 2023Quote: ... and 3-7 units/mL of pyruvate kinase and lactate dehydrogenase (Sigma P0294). An NADH standard curve was measured in order to convert absorbance values to the concentration of ATP hydrolyzed ...
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bioRxiv - Bioengineering 2023Quote: ... and creatine kinase (CK) levels were assessed using the Lactate Dehydrogenase Activity Assay Kit and Creatine Kinase Activity Assay Kit (Sigma), respectively ...
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bioRxiv - Physiology 2021Quote: ... activity of SDH was determined using a succinate dehydrogenase assay kit (Sigma-Aldrich, MAK197) following the manufacturers protocol using the mouse tibialis anterior (TA ...
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bioRxiv - Physiology 2022Quote: ... 2.4–4 units of pyruvate kinase/ 3.6–5.6 units lactate dehydrogenases enzymes (Sigma, P0294).
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bioRxiv - Biophysics 2023Quote: ... phosphoenol pyruvate (53 mM PEP, Beantown Chemical, 129745) and pyruvate kinase/lactate dehydrogenase enzymes (2.8% v/v PK/LDH, Sigma, P-0294). For experiments with PRC1 ...
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bioRxiv - Biochemistry 2022Quote: IDH activity was measured using the Isocitrate Dehydrogenase Activity Assay Kit (Sigma catalog no. MAK062) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Alcohol absorbance was measured using an enzymatic alcohol dehydrogenase assay (ADH-NAD kit; Sigma-Aldrich) per the manufacturer’s directions and as described previously49 ...
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bioRxiv - Biophysics 2023Quote: ... 4.5 μL pyruvate kinase/lactic dehydrogenase enzyme mixture from rabbit muscle (Sigma-P0294-5 mL) with 600 – 1000 units PK/mL and 900 – 1400 units LDH/mL ...
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bioRxiv - Physiology 2022Quote: ... triglyceride and glycerol (Triglyceride-SL Assay, Cat# 236-60, Sekisui Diagnostics, PEI, Canada) and pyruvate (Pyruvate Assay Kit, Cat#MAK071, Sigma-Aldrich, Oakville, ON, Canada) were measured using colorimetric assays according to the manufacturers’ directions ...
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bioRxiv - Microbiology 2020Quote: IDH activity assays were performed using the Isocitrate Dehydrogenase Activity Assay kit (MAK062) from Sigma-Aldrich (St. Louis, MS, USA) and monitored with a Synergy HT microplate reader (BioTek ...
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bioRxiv - Immunology 2021Quote: Succinate dehydrogenase activity was measured by MTT assay (Sigma, #M2128). Briefly ...
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bioRxiv - Physiology 2020Quote: ... Lactate dehydrogenase was measured in serum using a specific kit assay from Sigma (cat: MAK066, Merck). Pancreatic enzymatic activities ...
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bioRxiv - Biochemistry 2023Quote: ... Pyruvate kinase of rabbit muscle and Malate Dehydrogenase of pig heart muscle were purchased from Sigma. Protein concentrations were determined with via Bradford assay (Bio-Rad Protein Assay Dye Reagent).
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bioRxiv - Cell Biology 2023Quote: ... Neuronal cytotoxicity was determined with the Lactate Dehydrogenase Activity Assay Kit according to the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... LDH activity was measured using the Lactate Dehydrogenase Activity Assay Kit (Sigma-Aldrich, St. Louis, MO, USA). For histology ...
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bioRxiv - Biochemistry 2020Quote: ... was added to reaction mixtures containing Pyruvate Kinase and Lactic Dehydrogenase enzymes (Sigma-Aldrich, Gillingham, United Kingdom) with 0.010-0.016 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 50 mM.min-1 pyruvate kinase and 50 mM.min-1 lactate dehydrogenase (as assayed by the manufacturer, Sigma). Ligases were diluted prior to assay as required in 50 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM phosphoenolpyruvate (PEP) (Molecula, 16921512) and 16 units/ml lactic dehydrogenase/pyruvate kinase enzymes (Sigma, P0294). 30 mL reactions were prepared in 384 well plates (Greiner ...
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bioRxiv - Biochemistry 2022Quote: ... and commercial pyruvate kinase (PK) and lactate dehydrogenase (LDH) from Saccharomyces cerevisae (Sigma-Aldrich, Saint-Louis, USA) (Figure S2) ...
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bioRxiv - Plant Biology 2024Quote: ... both enzymes were combined with 10 units pyruvate kinase and 10 units lactate dehydrogenase (both Sigma-Aldrich) at 25°C ...
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bioRxiv - Biochemistry 2022Quote: Ammonium concentrations were determined using a glutamate dehydrogenase assay (Sigma-Aldrich) kit using the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Potential contamination among subcellular fractions was inspected by the presence of nucleus-specific marker Histone H3 on Western blot with human anti-Histone H3 antibody (ab1791) and mitochondria-specific activity of succinate dehydrogenase using the Succinate dehydrogenase activity colorimetric Assay (Sigma Aldrich), both performed according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... and 15 mM phosphoenolpyruvate in presence of a 4% v/v pyruvate kinase/lactate dehydrogenase enzyme mix (Sigma). Reactions were initiated by the addition of ATP to a final concentration of 25 μM - 6.4 mM ...