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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Protein reduction and alkylation and digestion with trypsin (Sigma-Aldrich) and LysC (WAKO ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was eluted by digestion with Factor Xa protease (Sigma). Factor Xa protease was removed using p-Aminobenzamidine-agarose (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... except an On-Column DNase I Digestion kit (Sigma) was used during RNA extraction.
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bioRxiv - Biophysics 2021Quote: CoA levels in the protein solution after proteinase K digestion were quantified using a commercial CoA assay kit according to manufacturer’s protocol (Sigma-Aldrich; MAK034). CoA concentration is determined by an enzymatic assay ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was based on the FASP procedure using 30K Amicon Ultra-4 filtration devices (Millipore). Briefly ...
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bioRxiv - Systems Biology 2021Quote: ... Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma) were used as external standards for absolute protein quantification (more details in Supp ...
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bioRxiv - Microbiology 2022Quote: ... followed by nucleic acid digestion with 0.5 Units of Benzonase per 1 µg of protein (Sigma-Aldrich) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Protein complexes were released from chromatin by digestion with 2 U/μl of Benzonase nuclease (E1014-25KU, Sigma) and sonicated for 5 min using a Diagenode sonicator with settings ...
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bioRxiv - Developmental Biology 2019Quote: ... in-gel tryptic digestion performed using proteoprofile trypsin in-gel digest kit (PP0100, Sigma-Aldrich). The samples were desalted using either ZipTip™ C18 (Millipore ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was removed using the On-Column DNase I digestion kit (Sigma-Aldrich, Sweden). cDNA was synthesised using the TATAA GrandScript cDNA Synthesis Kit according to the instructions of the manufacturer (TATAA Biocenter ...
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bioRxiv - Immunology 2022Quote: ... after digestion of the remaining genomic DNA by incubation with the “DNase I Kit” (Sigma) following the manufacturer’s manual ...
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bioRxiv - Systems Biology 2022Quote: ... The lysate was centrifuged at 4,000 xg for 5 min at 4°C and the protein pelletwas resuspended in Digestion Buffer(10% 2,2,2-Trifluoroethanol (Sigma; #96924)) in 100 mM HEPEs pH 8.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were resuspended in 150µL wash buffer 3 and diluted with two volumes of 10 mM Tris pH 8.5 prior to on-bead digestion of proteins via addition of 500 ng modified sequencing grade Trypsin (Sigma) with incubations of 1 hour at 4°C and then overnight at RT with continuous mixing at 1200 rpm ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Genetics 2022Quote: ... On-column DNase digestion was performed as described with On-Column DNaseI Digestion Set (Sigma Aldrich). For the 7 DAP reference ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed overnight at 37 °Cwith Lys-C (Wako) at an enzyme/protein ratio 1:75 and trypsin (Sigma) at an enzyme/protein ration of 1:50 ...
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bioRxiv - Cell Biology 2021Quote: ... and the dried protein pellet resuspended in digestion buffer (100 mM Tris-HCl pH 8.5, 1% sodium deoxycholate (Sigma-Aldrich), 5 mM TCEP ...
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bioRxiv - Developmental Biology 2020Quote: ... After digestion with hyaluronidase (Sigma Aldrich), MII oocytes were washed in sterile PBS and collected in RLT buffer (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... DNase digestion (Sigma, St. Louis, MO), and anti-BrdU antibody (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... DNase digestion (Sigma, St. Louis, MO), and anti-BrdU antibody (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... DNase digestion (Sigma, St. Louis, MO), and anti-BrdU antibody (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... DNase digestion (Sigma, St. Louis, MO), and anti-BrdU antibody (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... followed by digestion with Benzonase (Sigma) for digestion of RNA/DNA and lysed using a microfluidizer ...
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bioRxiv - Plant Biology 2023Quote: ... including on-column DNase digestion (Sigma). RNA integrity and quantity were determined by a Bioanalyzer RNA 6000 Nano assay (Agilent) ...
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bioRxiv - Plant Biology 2020Quote: ... DNA digestion was performed using the On-Column DNase I Digestion Set (Cat No.: DNASE70-1SET, Sigma) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... DNase digestion was performed on-column using the On-Column DNase I Digestion Set (Sigma Aldrich, DNASE70) as instructed ...
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bioRxiv - Cell Biology 2020Quote: ... BCA protein Assay Kit (Sigma-Aldrich) was used to quantify proteins in all the fractions and 30 µg of proteins per fraction were separated by SDS-PAGE for immunoblot.
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Cell Biology 2019Quote: ... Dehydration of gel pieces was done by addition of 100% ethanol followed by protein in-gel digestion with trypsin (Sigma-Aldrich) at 37 °C for 16 hrs ...
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bioRxiv - Plant Biology 2020Quote: ... MPK purification or modification (e.g., dephosphorylation or protease digestion) was followed by protein desalting using Amicon Ultra filters (Millipore, Billerica, MA) to remove HCO3- and other salts and buffers.
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bioRxiv - Biochemistry 2020Quote: ... followed by digestion with chymotrypsin (Sigma, # C6423) at 60:1 (w/w ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... infused with digestion buffer (RPMI 1640 [Sigma] ...
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bioRxiv - Neuroscience 2021Quote: ... Digestion with 1 μg trypsin (Sigma-Aldrich) per 50 μg peptide was done ON at RT.
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Unbiased transcriptomic analysis of chondrocyte differentiation in a high-density cell culture modelbioRxiv - Developmental Biology 2021Quote: ... Enzymatic digestion with Collagenase (3mg/ml, Sigma) and 0.25% Trypsin/EDTA (Invitrogen/Gibco BRL ...
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bioRxiv - Plant Biology 2021Quote: ... An on-column DNase digestion (Sigma-Aldrich) step was incorporated to remove genomic DNA ...
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bioRxiv - Genetics 2021Quote: ... followed by Xa protease digestion (Novagen, Inc.) and analyzed by SDS-PAGE.
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bioRxiv - Neuroscience 2024Quote: ... For thermolysin (TL) digestions (Sigma-Aldrich #T7902), brain extract at a concentration of 5 mg/mL was treated with 50 µg/mL protease (TL:protein ratio of 1:100 ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...