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Citations for Millipore Sigma :
1 - 50 of 5501 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... PCR products were purified with the High Pure PCR Product Purification Kit (Sigma Aldrich, 11732676001) and cloned into the pcDNA3.1 Hygro+ mammalian expression vector (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified using the GenElute PCR purification kit (Sigma-Aldrich, St. Louis, MO). The indexing PCR was carried out to attach the dual indices (Appendix D6.2 ...
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bioRxiv - Developmental Biology 2021Quote: ... All PCR reactions were column purified (High Pure PCR Product Purification kit, cat#11732668001, Sigma Aldrich). 2 ng/μl of purified PCR products were injected into either N2 or SM507 strains along with 100 ng/μl of pRF4 plasmid as a selection marker to create transgenic lines with extrachromosomal arrays.
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bioRxiv - Microbiology 2022Quote: ... followed by PCR product purification using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, U.S.A). All sequencing throughout was performed using the Macrogen sequencing service (Macrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA was purified using the High Pure PCR Product Purification Kit (11732676001 Sigma-Aldrich). Quantitative Real Time PCR reactions were performed with the TB Green™ Premix Ex Taq™ II (TAKARA ...
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bioRxiv - Synthetic Biology 2020Quote: ... Template plasmid was digested using DpnI and the PCR products were purified using a PCR purification kit (Sigma-Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... After purification with PCR Cleanup kit (Sigma-Aldrich), nucleic acids were eluted in 100 μL of elution buffer (5 mM Tris-HCl pH 8.5 ...
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bioRxiv - Genetics 2021Quote: ... All PCR products were purified with GenElute PCR Clean-Up Kit (Sigma) and sent for Sanger sequencing (Microsynth ...
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bioRxiv - Microbiology 2021Quote: ... PCR purification was performed using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Gibson assembly was done using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... PCR purification was conducted using GenElute™ PCR Clean-Up Kit (Sigma Aldrich) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and cDNA purification (Genelute PCR clean-up kit, Sigma-Aldrich) were carried out ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich), after restriction digestion of the PCR mixture with DpnI (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was purified by GenElute PCR Clean-Up Kit (Sigma-Aldrich) and cloned into pCR-Blunt II-TOPO vector by Zero Blunt TOPO PCR Cloning Kit (Invitrogen) ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified using the GenElute kit (Sigma Aldrich) and concentrations were checked using a NanoDrop ND1000 (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020). The expression vector pET302/NT-His was digested with EcoR1 restriction enzyme (NEB R0101S ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Samples were stored overnight at −20°C prior to library construction.
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bioRxiv - Genetics 2022Quote: ... all PCR products were amplified using Roche Expand High Fidelity PCR kit (Sigma 11732641001), which contains a 3’-adenine overhang-depositing Taq ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich, USA) followed by cloning of the same using pGEM-T Easy Vector System I ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) according to instruction manual ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) as per instruction manual and triplicates were pooled and diluted 1:5 for a second PCR with Illumina adapter sequences ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We purified the PCR product using the GenElute Plasmid Miniprep Kit (Sigma Aldrich) and used it as a template to insert the mutation into OmpF— transformed with pkD46 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were plate purified (Millipore Multiscreen filter plates ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product and pET-30b (+) (Novagen) were digested with XbaI and XhoI restriction enzymes ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) according to instruction manual ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2021Quote: ... The amplicons were purified using GFX PCR DNA and Gel Band Purification Kit (Sigma-Aldrich), according to the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... Libraries were purified using the High Pure PCR Production Purification Kit (11732676001, Roche / Sigma-Aldrich). Libraries were validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA purification was performed using a SpinPrep™ PCR Clean-up Kit (Merck Millipore, Germany). DNA fragments were assayed by qRT-PCR using the primer sequences listed below ...
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bioRxiv - Genetics 2019Quote: ... 50 uL of PCR product of each sample was purified with SpinPrep PCR Clean-Up Kit (EMD Millipore Corp) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Purification of genomic Polymerase Chain Reaction (PCR) amplified DNA was performed with the GenElute PCR Clean-Up kit (Sigma-Aldrich) or the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were cloned into pET28a (Novagen), pET28 His6 Sumo TEV LIC (1S ...
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bioRxiv - Immunology 2021Quote: ... PCR II products were purified by Millipore MultiScreen® PCRμ96 plate according to manufacture instructions and eluted in 30 μL of nuclease-free water (DEPC) ...
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bioRxiv - Immunology 2022Quote: ... The PCR products and pET16b vector (Novagen) were digested with NdeI and XhoI at 37°C for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and eluted with 15 μL of nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and sequenced by Sanger sequencing ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and sequenced by Sanger sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... A cDNA library was generated using the cDNA Library Kit from Thermo Fisher Scientific (catalog A48571) and the Reverse transcription PCR kit from Millipore Sigma (Product No. HSRT100), with specific primers were designed for each target gene (Supplementary Table 3) ...
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bioRxiv - Biophysics 2021Quote: ... The PCR products were cloned into pET21a (Novagen) using InFusion cloning technology ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were first cloned in pET22b (Novagen; containing the N-terminal pelB sequence ...
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bioRxiv - Microbiology 2023Quote: ... PCR product was cloned into vector pET28a (Novagen) and the obtained construct was used to transform E ...
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bioRxiv - Immunology 2023Quote: ... PCR II products were then purified by Millipore MultiScreen PCR 96 plate according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... PCR II products were then purified by Millipore MultiScreen PCR 96 plate according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR products were cloned into pET28 (Novagen) or pRSETA (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Molecular Biology 2022Quote: ... purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (Sigma-Aldrich, USA) and sent for Sanger sequencing (Source Bioscience ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were cloned into pET28a using infusion PCR (Novagen, Darmstadt, Germany). The recombinant vector was transformed into E ...
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bioRxiv - Cell Biology 2019Quote: PCR products were ligated into the p3XFLAGCMV10 (Sigma-Aldrich) and pEGFP-C1 (Clontech ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was ligated into pET28a(+) plasmid (Novagen), which was linearized with Nhe I and BamH I ...