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Citations for Millipore Sigma :
1 - 50 of 4796 citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... For genotyping PCR JumpStart RedTaq PCR master mix (Sigma-Aldrich) was used following the manufacturer’s protocol for cycling with an annealing temperature of 55°C and 35 cycles.
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using GFP primers ...
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using primers listed in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The genotyping process was conducted using the KAPA mouse genotyping kit (Millipore Sigma) using the following primer pairs ...
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bioRxiv - Immunology 2023Quote: ... using Mouse Kapa Genotyping kit (Merck-Millipore), primers (500 nM ...
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bioRxiv - Genomics 2020Quote: ... Primers used for genotyping PCR were synthesized by Sigma-Aldrich. Oligonucleotide sequences are given in Table 2.
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bioRxiv - Cancer Biology 2019Quote: ... Genotyping PCR was performed from genomic DNA from tails using standard protocol of N-Extract kit (Sigma).
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich) and respective primers ...
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bioRxiv - Cancer Biology 2019Quote: Genotyping to determine p53-/- mice was performed with the REDExtract-N-AMP Tissue PCR Kit (Sigma XNAT-100RXN). Ear punches were digested according to the kit protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Genotyping was verified by PCR (REDTaq® ReadyMix™ # R4775, Sigma Aldrich). The primers ...
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bioRxiv - Immunology 2020Quote: ... PCRs for genotyping of bacterial colonies after transformation were performed using the KAPA2G Fast ReadyMix kit (Sigma Aldrich, #KK5102) with custom designed primers and the following cycling conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... E11.5 embryos were dissected into ice-cold PBS and associated yolk sacs used for rapid genotyping using the Extract-N-Amp Tissue PCR kit as recommended by the manufacturer (Sigma). During genotyping ...
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bioRxiv - Developmental Biology 2023Quote: ... or ear biopsies (3 week old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was used for genotyping through REDTaq PCR Reaction Mix (Sigma Aldrich). Specific primer sets were applied to detect the CMV-Cre and IL-17A transgenes ...
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bioRxiv - Cell Biology 2022Quote: ... and extracted and amplified using the KAPA HotStartMouse Genotyping Kit (Sigma, KK7352). Genotyping primers for the WT allele were WT_FWD (5-ACTCCCAGCTCCAAGCTACA-3 ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: Genotyping of founders (F0) was done using genomic PCR with the primers following primers provided by Sigma, forward 5’-CGCGATGACAGTTTCCAGTA-3’ reverse 5’-CAAACAAAAACCCACTGAGGA-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with an equal volume of 40 mM Tris-HCl (pH 5.5).93 Multiplex polymerase chain reaction (PCR)-based genotyping was performed using REDTaq® polymerase per manufacturer recommendations (Millipore Sigma). Oligonucleotides were multiplexed as follows ...
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bioRxiv - Genetics 2023Quote: ... Our method of genotyping involved PCR amplification across mutation sites using the primers described in Table 1 (purchased from Sigma Aldrich). Then ...
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bioRxiv - Plant Biology 2019Quote: ... were sourced from Nottingham Arabidopsis Stock Centre and homozygous mutants where confirmed through genotyping PCR on genomic DNA using REDTaq® ReadyMix™ (Sigma) following their protocol ...
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bioRxiv - Genetics 2020Quote: PCR genotyping of all mouse strains was performed on genomic DNA obtained from tail biopsies digested with Proteinase K (Sigma, St. Louis, MO, USA) using primers listed in Table S1.
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bioRxiv - Developmental Biology 2020Quote: ... and genotyping was performed with NovaTaq Hot Start Master Mix (Millipore) according to the manufacturer’s cycling parameters (Tm=55°C ...
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bioRxiv - Cancer Biology 2021Quote: ... FastStart PCR Kit (Sigma-Aldrich) was used for PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... KAPA SYBR PCR kit (Sigma) was used for QPCR (Light Cycler 480 ...
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bioRxiv - Neuroscience 2023Quote: ... Genotyping was performed with REDExtract-N-Amp (Sigma Aldrich, St. Louis, MO, USA) using primers JAX oIMR9462 ATGCTCCAGACTGCCTTGGGAAAAG ...
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bioRxiv - Genetics 2021Quote: ... All PCR products were purified with GenElute PCR Clean-Up Kit (Sigma) and sent for Sanger sequencing (Microsynth ...
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bioRxiv - Pathology 2024Quote: Genotyping was performed as previously described 19 using the following primers (Sigma Aldrich; USA) for VHL floxed alleles ...
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bioRxiv - Neuroscience 2020Quote: ... The KAPA Taq PCR kit (Sigma, BK1000) was used in conjunction with three primer sequences (wild type ...
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bioRxiv - Plant Biology 2023Quote: ... the KAPA3G Plant PCR Kit (Sigma-Aldrich) was used to amplify an 824 bp fragment spanning the gene target site using an initial touchdown for 6 cycles with annealing at 70°C decreasing by 1.6°C per cycle followed by 34 cycles annealing at 62.1°C ...
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bioRxiv - Biochemistry 2023Quote: Mycoplasma PCR Detection Kit (LookOut, Sigma-Aldrich) Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The SYBR Green quantitative PCR kit (Sigma) was used with the following primers ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich), after restriction digestion of the PCR mixture with DpnI (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... PCR purification was performed using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Gibson assembly was done using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was purified by GenElute PCR Clean-Up Kit (Sigma-Aldrich) and cloned into pCR-Blunt II-TOPO vector by Zero Blunt TOPO PCR Cloning Kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... PCR purification was conducted using GenElute™ PCR Clean-Up Kit (Sigma Aldrich) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020). The expression vector pET302/NT-His was digested with EcoR1 restriction enzyme (NEB R0101S ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Samples were stored overnight at −20°C prior to library construction.
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bioRxiv - Genomics 2021Quote: PCR barcoding was performed using Kapa 2G Robust PCR kit (Sigma Catalogue No. KK5005) as follows ...
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bioRxiv - Genetics 2022Quote: ... all PCR products were amplified using Roche Expand High Fidelity PCR kit (Sigma 11732641001), which contains a 3’-adenine overhang-depositing Taq ...
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bioRxiv - Cell Biology 2020Quote: ... After purification with PCR Cleanup kit (Sigma-Aldrich), nucleic acids were eluted in 100 μL of elution buffer (5 mM Tris-HCl pH 8.5 ...
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: ... SybrGreen Quantitative RT-PCR kit (Sigma Aldrich, Germany) was used with Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Microbiology 2022Quote: The KAPA Taq PCR Kit (Sigma-Aldrich KR0352_S) was used to amplify the reads from a cDNA mixture of uninfected samples and 1 ...
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bioRxiv - Cell Biology 2023Quote: ... The LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) was used to assure that all cell lines were mycoplasma-free ...
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bioRxiv - Microbiology 2024Quote: ... purified (GenElute PCR Clean-up kit, Sigma Aldrich) and quantified using a Qubit Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich, USA) followed by cloning of the same using pGEM-T Easy Vector System I ...
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bioRxiv - Developmental Biology 2022Quote: ... Several probes were produced by PCR amplification (KAPA2G Fast ReadyMix PCR kit, Sigma Israel, KK5101). cDNA templates were synthesized by RNA precipitation followed by reverse transcription PCR ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... PCR products were purified with the High Pure PCR Product Purification Kit (Sigma Aldrich, 11732676001) and cloned into the pcDNA3.1 Hygro+ mammalian expression vector (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... For gene expression analysis one-step qRT-PCR (SYBR Green Quantitative RT-PCR kit, Sigma) was carried out ...
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bioRxiv - Cancer Biology 2020Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).