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Citations for Millipore Sigma :
1 - 50 of 6670 citations for Nucleic Acid Modification since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Viral nucleic acids or HT-DNA (Sigma) were transfected into MDDCs or PMA-differentiated THP1 using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Nucleic acid oligonucleotides (Sigma-Aldrich, HPLC-grade) used included ...
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bioRxiv - Microbiology 2023Quote: ... nucleic acids (salmon sperm DNA; Sigma-Aldrich, Deoxyribonucleic acid sodium salt) and a membrane lipid (L-α-phosphatidylethanolamine ...
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bioRxiv - Bioengineering 2021Quote: ... Nucleic acids were digested using Benzonase nuclease (Sigma) at 37 °C for 1 h followed by 10 min boiling to inactivate the enzyme ...
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bioRxiv - Microbiology 2022Quote: ... a live cell-impermeant nucleic acid dye (Sigma). Plates were then incubated in the CLARIOstar Plus plate reader (BMG Labtech ...
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bioRxiv - Genetics 2023Quote: ... and nucleic acid staining with DAPI (Sigma-Aldrich). Tissues were fixed in 4% PFA (Electron Microscopy Science ...
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bioRxiv - Molecular Biology 2020Quote: The following nucleic acid oligonucleotides (Sigma-Aldrich, HPLC-grade) were used for the transcription scaffold containing a 12-nt mismatch that was freshly assembled prior to cryo-EM sample preparation of elongating Pol III (see below) ...
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bioRxiv - Plant Biology 2021Quote: ... Sybr Green I nucleic acid gel stain (Sigma-Aldrich) was used for detection ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleic acids were digested with 85U benzonase (Sigma-Aldrich) per 100 × 106 cells ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acids were precipitated by addition of 0.1% polyethylenimine branched (SIGMA). Crude cell lysates were cleared by centrifugation at 200,000 × g for 1 hour at 4 °C in a Beckman Optima L-70 ultracentrifuge ...
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β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: ... free nucleic acids were digested with Benzonase® Nuclease (70746, Millipore) and leftover cellular components were discarded after centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were degraded using benzonase nuclease (Sigma-Aldrich, MO, USA) and samples were clarified by centrifugation at 10 000xg for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... Nucleic acids were digested with 15 units micrococcal nuclease (Sigma Aldrich) for 20 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The fish nucleic acids were degraded by 200 units Benzonase (Sigma, USA), 6 units of Turbo™ DNase (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Nucleic acid was degraded enzymatically with 0.125 U/µg benzonase (Sigma-Aldrich), and protein concentration was determined by Bradford assay (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... total nucleic acids were extracted from polycarbonate filters (Millipore, 0.2 μm pore size) following Nercessian et al ...
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bioRxiv - Cancer Biology 2023Quote: ... nucleic acid solutions were contrasted with 0.01% Fast Green solution in PBS (Sigma) to control the injection site carefully ...
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bioRxiv - Molecular Biology 2023Quote: ... Nucleic acids were purified with phenol/chloroform/isoamylic alcohol 25:24:1 (Sigma) and then precipitated overnight by adding Glycoblue (Thermofischer ...
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bioRxiv - Cell Biology 2023Quote: ... and nucleic acids were stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Stained parasites on the coverslips were mounted using Immu-mount (Fisher 9990402) ...
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bioRxiv - Cell Biology 2022Quote: ... Nucleic acids were stained with Hoechst-33342 (Sigma Aldrich, B2883, 1:2000 in PBS) for 20 min at RT ...
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bioRxiv - Biophysics 2021Quote: ... Nucleic acids were digested by adding MgCl2 to 2.5 mM and Benzonase (EMD Millipore) to ∼12.5 units per 1 ml lysate ...
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bioRxiv - Genomics 2020Quote: ... Nucleic acids were randomly amplified using the WTA2 kit (WTA2, Sigma-Aldrich, Darmstadt, Germany) and libraries were prepared using the Illumina Nextera XT kit (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich) using a QuantStudio 5 Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were mounted in Fluoroshield supplemented with the nucleic acid stain DAPI (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... DNA was then transferred from gel to charged nylon for nucleic acid blotting (Millipore) followed by cross-linking using a UV Stratalinker from Stratagene and detected using LightShift™ Chemiluminescent EMSA Kit (Thermo Scientific™
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bioRxiv - Microbiology 2023Quote: ... Bacterial cell death was assessed using SYTOX™ green nucleic acid stain (S7020, Sigma). 0.3-1 µl of 5 mM stain was added to the sample and images acquired ...
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bioRxiv - Microbiology 2023Quote: ... nucleic acids were randomly amplified using the Whole Transcriptome Amplification kit (WTA2, Sigma Aldrich) with modifications (Conceição-Neto et al. ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, 1 µg/mL) and the cells were washed three times with PBS ...
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bioRxiv - Physiology 2023Quote: ... Peterson’s Modification (Sigma TP0300). The plate was incubated at room temperature and was read at 750 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... washed three times with PTw and counterstained for nucleic acid with DAPI (0.004% v/v) (Sigma) for five minutes followed by three washes in PTw ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA:DNA hybrids were immunoprecipitated from total nucleic acids using 5µg of S9.6 antibody (MABE1095, Merck Millipore) in binding buffer (10mM Na2HPO4 pH 7.0 ...
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bioRxiv - Developmental Biology 2022Quote: ... Nucleic acids were precipitated by adding and equal volume of 5 M Ammonium Acetate (Sigma-Aldrich), 2.5 volumes 100% ethanol and precipitated for >1 hour at −80°C ...
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bioRxiv - Genomics 2020Quote: ... We extracted nucleic acids with phenol/chloroform/isoamyl alcohol (25:24:1) pH 8.0 (Sigma-Aldrich, USA) using 2ml light Phase lock Gels (5Prime ...
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bioRxiv - Microbiology 2021Quote: ... The extracted nucleic acids were further treated with 1 μL of RNase A solution (#R6148, Sigma Aldrich) for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... followed by nucleic acid digestion with 0.5 Units of Benzonase per 1 µg of protein (Sigma-Aldrich) for 30 min at 37°C ...
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Stromal transdifferentiation drives lymph node lipomatosis and induces extensive vascular remodelingbioRxiv - Pathology 2022Quote: ... alpha modification media (Sigma Aldrich) supplemented with 1% L-glutamine (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... samples were stained with the fluorescent nucleic acid marker 4,6-diamino-2-fenilindol (DAPI 1:3000; Sigma-Aldrich), and mounted with a Fluorescent Mounting Medium (DAKO ...
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bioRxiv - Synthetic Biology 2021Quote: ... nucleic acids were precipitated with an equal volume of isopropanol and then treated with RNase A (Sigma‐ Aldrich) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... with 1:30,000 of the fluorescent marker of nucleic acids 4’,6-diamino-2-fenilindol (DAPI; Sigma-Aldrich). We took photographs of the cells using an epifluorescence microscope (DMIL LED ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleic acids from the protein samples were removed by incubating the samples with Benzonase (Millipore Sigma, Cat. No.E1014) for 20 min on ice before use in Sun1 interacting pulldown assay.
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bioRxiv - Genomics 2023Quote: ... We first extracted nucleic acids with phenol/chloroform/isoamyl alcohol (25:24:1) pH 8.0 (Sigma-Aldrich, USA) three times using 1.5ml light Phase lock Gels (5Prime ...
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bioRxiv - Cell Biology 2024Quote: ... 200 mM Tris-HCl pH 7.4) to later stretch the nucleic acid fibers on silanized slides (Sigma, S4651), tilting the slides to a 15° ...
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bioRxiv - Cell Biology 2022Quote: ... MEM alpha modification medium (Sigma-Aldrich), 1.25 mL Glutamax (Gibco) ...
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bioRxiv - Physiology 2023Quote: ... Micro Lowry,Peterson’s Modification: (Sigma TP0300). The triglycerides were determined using Serum Triglyceride Determination Kit (Sigma TR0100 ...
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bioRxiv - Plant Biology 2020Quote: ... with the following modification: guard cell lysis was performed by adding glass beads (425 μm - 600 μm diameter, acid washed, from Sigma-Aldrich) and 350 μl RULT buffer to the sample ...
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bioRxiv - Biophysics 2019Quote: ... Cell death of bacteria was assessed by adding 1 μL of SYTOX™ green nucleic acid stain (S7020, Sigma) to the sample to mark dead cells ...
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bioRxiv - Neuroscience 2022Quote: ... All sections were treated with an RNP-stabilizing blocking solution [1% blocking reagent for nucleic acid hybridization (Sigma 11096176001) in PBST (PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were lysed by sonication and nucleic acids were digested by incubating with Benzonase Nuclease (125 units, Sigma) for 30 min at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA was visualised under UV light following staining for 30 minutes with 0.5 µg ml-1 GelRed Nucleic Acid Stain (Millipore) and destaining in deionised water for 1 hour.
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bioRxiv - Genetics 2020Quote: ... the Imprint DNA Modification Kit (Sigma Aldrich) was used for bisulfite treatment on 200ng DNA ...