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Citations for Millipore Sigma :
1 - 50 of 8880 citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Sybr Green I nucleic acid gel stain (Sigma-Aldrich) was used for detection ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich) using a QuantStudio 5 Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was then transferred from gel to charged nylon for nucleic acid blotting (Millipore) followed by cross-linking using a UV Stratalinker from Stratagene and detected using LightShift™ Chemiluminescent EMSA Kit (Thermo Scientific™
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bioRxiv - Microbiology 2019Quote: Viral nucleic acids or HT-DNA (Sigma) were transfected into MDDCs or PMA-differentiated THP1 using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Nucleic acid oligonucleotides (Sigma-Aldrich, HPLC-grade) used included ...
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bioRxiv - Microbiology 2023Quote: ... nucleic acids (salmon sperm DNA; Sigma-Aldrich, Deoxyribonucleic acid sodium salt) and a membrane lipid (L-α-phosphatidylethanolamine ...
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bioRxiv - Bioengineering 2021Quote: ... Nucleic acids were digested using Benzonase nuclease (Sigma) at 37 °C for 1 h followed by 10 min boiling to inactivate the enzyme ...
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bioRxiv - Microbiology 2022Quote: ... a live cell-impermeant nucleic acid dye (Sigma). Plates were then incubated in the CLARIOstar Plus plate reader (BMG Labtech ...
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bioRxiv - Genetics 2023Quote: ... and nucleic acid staining with DAPI (Sigma-Aldrich). Tissues were fixed in 4% PFA (Electron Microscopy Science ...
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bioRxiv - Molecular Biology 2020Quote: The following nucleic acid oligonucleotides (Sigma-Aldrich, HPLC-grade) were used for the transcription scaffold containing a 12-nt mismatch that was freshly assembled prior to cryo-EM sample preparation of elongating Pol III (see below) ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleic acids were digested with 85U benzonase (Sigma-Aldrich) per 100 × 106 cells ...
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bioRxiv - Biophysics 2019Quote: ... PCR reactions were analyzed by gel electrophoresis using a 1% agarose gel in TAE buffer (20 mM acetic acid (Sigma Aldrich, cat#, 695092), 2 mM EDTA (ACROS Organics ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA reaction was diluted 1:10 in water and used for qPCR analysis with primers directed against BR marker genes EXPA8 and DWF4 or against RLP44.The SYBRR Green I nucleic acid gel stain (Sigma-Aldrich) was used for detection ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acids were precipitated by addition of 0.1% polyethylenimine branched (SIGMA). Crude cell lysates were cleared by centrifugation at 200,000 × g for 1 hour at 4 °C in a Beckman Optima L-70 ultracentrifuge ...
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β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: ... free nucleic acids were digested with Benzonase® Nuclease (70746, Millipore) and leftover cellular components were discarded after centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were degraded using benzonase nuclease (Sigma-Aldrich, MO, USA) and samples were clarified by centrifugation at 10 000xg for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... Nucleic acids were digested with 15 units micrococcal nuclease (Sigma Aldrich) for 20 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The fish nucleic acids were degraded by 200 units Benzonase (Sigma, USA), 6 units of Turbo™ DNase (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Nucleic acid was degraded enzymatically with 0.125 U/µg benzonase (Sigma-Aldrich), and protein concentration was determined by Bradford assay (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Both 1:1 and 1:10 dilutions of each sample were prepared in duplicate with 0.5X SYBR Green I nucleic acid gel stain (Sigma-Aldrich, St. Louis, MO), plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: ... Both 1:1 and 1:10 dilutions of each sample were prepared in duplicate with 0.5X SYBR Green I nucleic acid gel stain (Sigma-Aldrich, St. Louis, MO), plus 280 nM each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs ...
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bioRxiv - Neuroscience 2021Quote: ... Gel electrophoresis was performed with 1.8% agarose gels (VWR Life science, Sigma- Aldrich) in 1X TAE buffer (40 mM Tris ...
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bioRxiv - Biophysics 2022Quote: ... before electrophoresis on a 1.3% agarose gel (Sigma Aldrich) stained with gelstar (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... gel electrophoresis and transferred to PVDF membranes (Millipore, IPFL00010). Membranes were blocked with Odyssey blocking buffer (Li-Cor ...
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bioRxiv - Microbiology 2022Quote: ... total nucleic acids were extracted from polycarbonate filters (Millipore, 0.2 μm pore size) following Nercessian et al ...
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bioRxiv - Cancer Biology 2023Quote: ... nucleic acid solutions were contrasted with 0.01% Fast Green solution in PBS (Sigma) to control the injection site carefully ...
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bioRxiv - Molecular Biology 2023Quote: ... Nucleic acids were purified with phenol/chloroform/isoamylic alcohol 25:24:1 (Sigma) and then precipitated overnight by adding Glycoblue (Thermofischer ...
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bioRxiv - Cell Biology 2023Quote: ... and nucleic acids were stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Stained parasites on the coverslips were mounted using Immu-mount (Fisher 9990402) ...
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bioRxiv - Cell Biology 2022Quote: ... Nucleic acids were stained with Hoechst-33342 (Sigma Aldrich, B2883, 1:2000 in PBS) for 20 min at RT ...
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bioRxiv - Biophysics 2021Quote: ... Nucleic acids were digested by adding MgCl2 to 2.5 mM and Benzonase (EMD Millipore) to ∼12.5 units per 1 ml lysate ...
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bioRxiv - Genomics 2020Quote: ... Nucleic acids were randomly amplified using the WTA2 kit (WTA2, Sigma-Aldrich, Darmstadt, Germany) and libraries were prepared using the Illumina Nextera XT kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Samples were mounted in Fluoroshield supplemented with the nucleic acid stain DAPI (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... nucleic acids were randomly amplified using the Whole Transcriptome Amplification kit (WTA2, Sigma Aldrich) with modifications (Conceição-Neto et al. ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cell death was assessed using SYTOX™ green nucleic acid stain (S7020, Sigma). 0.3-1 µl of 5 mM stain was added to the sample and images acquired ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, 1 µg/mL) and the cells were washed three times with PBS ...
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bioRxiv - Biophysics 2020Quote: ... and subjected to agarose gel electrophoresis (1% agarose (A9539, Sigma Aldrich), 1x Tris Acetate-EDTA (TAE ...
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bioRxiv - Microbiology 2021Quote: ... proteins were fractionated on SDS-polyacrylamide gel electrophoresis gel and transferred to a nitrocellulose membrane (Millipore). Membranes were incubated with primary antibodies ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR fragments were analyzed using Agarose Gel Electrophoresis and purified from Gel using the GenElute Gel Extraction Kit (Sigma, NA1111) or MinElute Gel Extraction Kit (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... washed three times with PTw and counterstained for nucleic acid with DAPI (0.004% v/v) (Sigma) for five minutes followed by three washes in PTw ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA:DNA hybrids were immunoprecipitated from total nucleic acids using 5µg of S9.6 antibody (MABE1095, Merck Millipore) in binding buffer (10mM Na2HPO4 pH 7.0 ...
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bioRxiv - Developmental Biology 2022Quote: ... Nucleic acids were precipitated by adding and equal volume of 5 M Ammonium Acetate (Sigma-Aldrich), 2.5 volumes 100% ethanol and precipitated for >1 hour at −80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR products were separated by gel electrophoresis in 1% agarose (Sigma). Gene-Ruler 100 bp DNA ladder (ThermoScientific ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were analysed by electrophoresis on 1% agarose gel (Sigma-Aldrich), containing SafeView (ABM) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The digested plasmids were checked by agarose gel electrophoresis (1% agarose (Sigma), 1xTris-boric acid EDTA buffer ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity was checked in 1% agarose gel electrophoresis (Sigma-Aldrich A9539) and concentration and purity were assessed with a Nanodrop ND-100 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Biophysics 2023Quote: ... the clicked and hybridized samples were purified by gel electrophoresis using a 1.5% agarose gel (Sigma, A9539-500G) in 1× TBE buffer ...
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bioRxiv - Genomics 2020Quote: ... We extracted nucleic acids with phenol/chloroform/isoamyl alcohol (25:24:1) pH 8.0 (Sigma-Aldrich, USA) using 2ml light Phase lock Gels (5Prime ...
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bioRxiv - Microbiology 2021Quote: ... The extracted nucleic acids were further treated with 1 μL of RNase A solution (#R6148, Sigma Aldrich) for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... followed by nucleic acid digestion with 0.5 Units of Benzonase per 1 µg of protein (Sigma-Aldrich) for 30 min at 37°C ...